##################################
# NO CUT ENZYMES ( 66 enzymes) #
##################################
AatII AclI AflII AocI Aor51HI
ApaI AscI AviII AvrII BbrPI
BfrI BglI BlnI BsaAI BsaBI
BsiWI Bsp1407I BspLU11I BsrBI BsrGI
BssHII BssSI Bst1107I BstAPI BstEII
BstPI BstZ17I Bsu36I CpoI Eco47III
Eco81I EcoNI EcoO65I FseI FspI
HindIII KspI MamI NaeI NgoMIV
NheI NotI NruI PaeR7I PmaCI
PmeI PmlI PshAI Psp1406I PvuI
RsrII SacII SbfI ScaI SexAI
SfiI SgrAI SmlI SnaBI SpeI
SphI Sse8387I SspBI StuI SwaI
XhoI
###################################
# ONE CUT ENZYMES ( 56 enzymes) #
###################################
Acc65I 4015 GGTACC G^GTACC
AccII 2533 CGCG CG^CG
AccIII 552 TCCGGA T^CCGGA
AflIII 2532 ACGCGT A^C[GA][CT]GT
AgeI 3477 ACCGGT A^CCGGT
Alw44I 2677 GTGCAC G^TGCAC
ApaLI 2677 GTGCAC G^TGCAC
AseI 4719 ATTAAT AT^TAAT
AsnI 4719 ATTAAT AT^TAAT
Asp718I 4015 GGTACC G^GTACC
BalI 1639 TGGCCA TGG^CCA
BamHI 2517 GGATCC G^GATCC
BbeI 3279 GGCGCC GGCGC^C
BglII 3587 AGATCT A^GATCT
BlpI 3871 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 3871 GCTGAGC GC^T[ACGT]AGC
BseAI 552 TCCGGA T^CCGGA
BspDI 506 ATCGAT AT^CGAT
BspEI 552 TCCGGA T^CCGGA
BspHI 882 TCATGA T^CATGA
BstBI 705 TTCGAA TT^CGAA
BstUI 2533 CGCG CG^CG
BstXI 2221 CCAAGATGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 3871 GCTGAGC GC^T[ACGT]AGC
ClaI 506 ATCGAT AT^CGAT
DraII 3022 GGGTCCT [GA]G^G[ACGT]CC[CT]
EcoO109I 3022 GGGTCCT [GA]G^G[ACGT]CC[CT]
HpaI 532 GTTAAC GTT^AAC
KasI 3279 GGCGCC G^GCGCC
KpnI 4015 GGTACC GGTAC^C
MfeI 4943 CAATTG C^AATTG
MluI 2532 ACGCGT A^CGCGT
MluNI 1639 TGGCCA TGG^CCA
MroI 552 TCCGGA T^CCGGA
MscI 1639 TGGCCA TGG^CCA
MunI 4943 CAATTG C^AATTG
MvnI 2533 CGCG CG^CG
NarI 3279 GGCGCC GG^CGCC
NcoI 4840 CCATGG C^CATGG
NdeI 3116 CATATG CA^TATG
NspV 705 TTCGAA TT^CGAA
PacI 4624 TTAATTAA TTAAT^TAA
PinAI 3477 ACCGGT A^CCGGT
PpuMI 3022 GGGTCCT [GA]G^G[AT]CC[CT]
PshBI 4719 ATTAAT AT^TAAT
PstI 2584 CTGCAG CTGCA^G
RcaI 882 TCATGA T^CATGA
SacI 4224 GAGCTC GAGCT^C
SalI 3702 GTCGAC G^TCGAC
SapI 2777 GCTCTTC GCTCTTC(1/4)
SfoI 3279 GGCGCC GGC^GCC
SfuI 705 TTCGAA TT^CGAA
SmaI 1816 CCCGGG CCC^GGG
XcmI 3805 CCATAACACTAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 1816 CCCGGG C^CCGGG
XmaI 1816 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
AccI 375 GTAGAC GT^[AC][GT]AC
3702 GTCGAC
AcyI 1946 GGCGTC G[GA]^CG[CT]C
3279 GGCGCC
AhdI 2092 GACAATCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3374 GACCCCTTGTC
AspEI 2092 GACAATCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3374 GACCCCTTGTC
AspI 746 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2601 GACTCTGTC
AvaI 1816 CCCGGG C^[CT]CG[GA]G
4182 CCCGAG
BanI 3279 GGCGCC G^G[CT][GA]CC
4015 GGTACC
BbvCI 496 CCTCAGC CCTCAGC(-5/-2)
1738 CCTCAGC
BciVI 3427 GGATAC GTATCC(6/5)
4022 GGATAC
BclI 2988 TGATCA T^GATCA
3313 TGATCA
BsaHI 1946 GGCGTC G[GA]^CG[CT]C
3279 GGCGCC
BsaI 1031 GAGACC GGTCTC(1/5)
3647 GAGACC
BsgI 2803 GTGCAG GTGCAG(16/14)
3672 GTGCAG
BsiEI 401 CGGCCG CG[GA][CT]^CG
3522 CGGCCG
BsmBI 388 CGTCTC CGTCTC(1/5)
3850 GAGACG
BsoBI 1816 CCCGGG C^[CT]CG[GA]G
4182 CCCGAG
BsrDI 1807 CATTGC GCAATG(2/0)
5044 GCAATG
DraIII 671 CACGCAGTG CAC[ACGT][ACGT][ACGT]^GTG
2141 CACACTGTG
DrdI 583 GACCTTCTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2417 GACAGAACGGTC
DsaI 436 CCACGG C^C[GA][CT]GG
4840 CCATGG
EagI 401 CGGCCG C^GGCCG
3522 CGGCCG
Eam1105I 2092 GACAATCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3374 GACCCCTTGTC
EclXI 401 CGGCCG C^GGCCG
3522 CGGCCG
Eco52I 401 CGGCCG C^GGCCG
3522 CGGCCG
EcoRI 143 GAATTC G^AATTC
943 GAATTC
EcoRV 2363 GATATC GAT^ATC
4246 GATATC
EcoT22I 3049 ATGCAT ATGCA^T
4066 ATGCAT
FauI 1800 CCCGC CCCGC(4/6)
2459 GCGGG
FbaI 2988 TGATCA T^GATCA
3313 TGATCA
HgaI 479 GACGC GACGC(5/10)
1947 GCGTC
Hin1I 1946 GGCGTC G[GA]^CG[CT]C
3279 GGCGCC
NsiI 3049 ATGCAT ATGCA^T
4066 ATGCAT
PflFI 746 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2601 GACTCTGTC
PflMI 1621 CCACCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3947 CCAGAGACTGG
Tth111I 746 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2601 GACTCTGTC
Van91I 1621 CCACCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3947 CCAGAGACTGG
XbaI 173 TCTAGA T^CTAGA
2662 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 99 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI Asp700I AspHI AvaII
BanII BbsI BbvI BcgI BcnI
BfaI BmyI BpmI BpuAI BsaJI
BsaWI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I BspMI
BsrFI BsrI BssKI BstF5I BstNI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraI EaeI EarI
EcoRII EcoT14I Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII XmnI