##################################
# NO CUT ENZYMES ( 93 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AgeI AhdI Alw44I Aor51HI ApaLI
AscI Asp718I AspEI AspI AviII
AvrII BbeI BbrPI BciVI BcnI
BglII BlnI BlpI Bpu1102I BsaAI
BsaHI BsaWI BseAI BsiWI BspEI
BspHI BspLU11I BspMI BsrBI BsrFI
BssHII BstBI BstEII BstPI CelII
Cfr10I CpoI DraIII DrdI Eam1105I
Eco47III EcoNI EcoO65I FseI FspI
HgaI Hin1I KasI KpnI KspI
MfeI MluI MroI MunI NaeI
NarI NciI NdeI NgoMIV NotI
NruI NspV PacI PflFI PflMI
PinAI PmaCI PmeI PmlI Psp1406I
PvuI RcaI RsrII SacII SalI
SbfI SfiI SfoI SfuI SgrAI
SmaI SnaBI Sse8387I StuI Tth111I
Van91I XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AccI 3206 GTATAC GT^[AC][GT]AC
AccII 3808 CGCG CG^CG
AflII 4376 CTTAAG C^TTAAG
AflIII 4398 ACACGT A^C[GA][CT]GT
ApaI 3252 GGGCCC GGGCC^C
BanI 4157 GGCACC G^G[CT][GA]CC
BclI 674 TGATCA T^GATCA
BfrI 4376 CTTAAG C^TTAAG
BsaBI 4975 GATGGATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 4607 CGGCCG CG[GA][CT]^CG
BsmBI 3547 CGTCTC CGTCTC(1/5)
BsmI 4253 GCATTC GAATGC(1/-1)
Bsp1407I 4596 TGTACA T^GTACA
BsrGI 4596 TGTACA T^GTACA
Bst1107I 3206 GTATAC GTA^TAC
BstAPI 2218 GCAGACGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 3808 CGCG CG^CG
BstXI 4411 CCAACTCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 3206 GTATAC GTA^TAC
DraI 432 TTTAAA TTT^AAA
EagI 4607 CGGCCG C^GGCCG
EclXI 4607 CGGCCG C^GGCCG
Eco52I 4607 CGGCCG C^GGCCG
EcoT22I 2225 ATGCAT ATGCA^T
FauI 3613 CCCGC CCCGC(4/6)
FbaI 674 TGATCA T^GATCA
HapII 4606 CCGG C^CGG
HpaI 4922 GTTAAC GTT^AAC
HpaII 4606 CCGG C^CGG
MamI 4975 GATGGATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspI 4606 CCGG C^CGG
MvnI 3808 CGCG CG^CG
NsiI 2225 ATGCAT ATGCA^T
PaeR7I 4145 CTCGAG C^TCGAG
PstI 1105 CTGCAG CTGCA^G
SacI 1920 GAGCTC GAGCT^C
ScaI 4725 AGTACT AGT^ACT
SexAI 4114 ACCTGGT A^CC[AT]GGT
SspBI 4596 TGTACA T^GTACA
SwaI 431 ATTTAAAT ATTT^AAAT
XhoI 4145 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AocI 927 CCTTAGG CC^T[ACGT]AGG
3578 CCTCAGG
AspHI 1920 GAGCTC G[AT]GC[AT]^C
3662 GAGCAC
BbvCI 141 CCTCAGC CCTCAGC(-5/-2)
1136 CCTCAGC
BcgI 2801 CGATTATCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1337 GCATTTGACTCG
BglI 896 GCCGTCTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2237 GCCAAAGTGGC
BsaI 1359 GAGACC GGTCTC(1/5)
1673 GAGACC
BsiHKAI 1920 GAGCTC G[AT]GC[AT]^C
3662 GAGCAC
BspDI 2548 ATCGAT AT^CGAT
2847 ATCGAT
BssSI 840 CTCGTG CACGAG(-5/-1)
4569 CTCGTG
Bsu36I 927 CCTTAGG CC^T[ACGT]AGG
3578 CCTCAGG
ClaI 2548 ATCGAT AT^CGAT
2847 ATCGAT
Eco81I 927 CCTTAGG CC^T[ACGT]AGG
3578 CCTCAGG
HaeII 1887 GGCGCT [GA]GCGC^[CT]
4192 AGCGCC
NcoI 288 CCATGG C^CATGG
4291 CCATGG
NheI 1963 GCTAGC G^CTAGC
3494 GCTAGC
PshAI 657 GACAGATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1227 GACCTTTGTC
PvuII 775 CAGCTG CAG^CTG
3626 CAGCTG
SapI 3638 GAAGAGC GCTCTTC(1/4)
2073 GCTCTTC
SfcI 1105 CTGCAG C^T[GA][CT]AG
1878 CTGTAG
SpeI 583 ACTAGT A^CTAGT
4613 ACTAGT
SphI 1837 GCATGC GCATG^C
4457 GCATGC
SspI 2443 AATATT AAT^ATT
5034 AATATT
XbaI 697 TCTAGA T^CTAGA
4138 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 101 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AseI AsnI Asp700I
AvaI AvaII BalI BamHI BanII
BbsI BbvI BfaI BmyI BpmI
BpuAI BsaJI BseRI BsgI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrDI BsrI BssKI BstF5I BstNI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoO109I EcoRI EcoRII EcoRV EcoT14I
Fnu4HI FokI HaeIII HhaI HinP1I
HincII HindII HindIII HinfI HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MvaI
MwoI NdeII NlaIII NlaIV NspI
PleI PpuMI PshBI RsaI Sau3AI
Sau96I ScrFI SfaNI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XhoII
XmnI