##################################
# NO CUT ENZYMES ( 52 enzymes) #
##################################
AatII AhdI AscI AspEI AspI
AviII BamHI BbrPI BcgI BciVI
BglI BsaAI BsiEI BsiWI BspDI
BssHII BssSI Bst1107I BstBI BstZ17I
ClaI CpoI DrdI EagI Eam1105I
EclXI Eco52I EcoRV FseI FspI
HpaI MluI NheI NotI NruI
NspV PacI PflFI PmaCI PmeI
PmlI PvuI RsrII SalI SapI
ScaI SexAI SfiI SfuI SgrAI
SnaBI Tth111I
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
Acc65I 3660 GGTACC G^GTACC
AccI 4907 GTAGAC GT^[AC][GT]AC
AccIII 76 TCCGGA T^CCGGA
AclI 2908 AACGTT AA^CGTT
AgeI 6053 ACCGGT A^CCGGT
Aor51HI 42 AGCGCT AGC^GCT
ApaI 3427 GGGCCC GGGCC^C
Asp718I 3660 GGTACC G^GTACC
AvrII 6362 CCTAGG C^CTAGG
BalI 1201 TGGCCA TGG^CCA
BbvCI 2270 GCTGAGG CCTCAGC(-5/-2)
BlnI 6362 CCTAGG C^CTAGG
BlpI 396 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 396 GCTCAGC GC^T[ACGT]AGC
BsaBI 2515 GATATGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 76 TCCGGA T^CCGGA
BsmBI 7023 CGTCTC CGTCTC(1/5)
Bsp1407I 6588 TGTACA T^GTACA
BspEI 76 TCCGGA T^CCGGA
BsrGI 6588 TGTACA T^GTACA
CelII 396 GCTCAGC GC^T[ACGT]AGC
DraIII 631 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 42 AGCGCT AGC^GCT
KpnI 3660 GGTACC GGTAC^C
KspI 6 CCGCGG CCGC^GG
MamI 2515 GATATGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1201 TGGCCA TGG^CCA
MroI 76 TCCGGA T^CCGGA
MscI 1201 TGGCCA TGG^CCA
NaeI 59 GCCGGC GCC^GGC
NcoI 3556 CCATGG C^CATGG
NgoMIV 59 GCCGGC G^CCGGC
PaeR7I 786 CTCGAG C^TCGAG
PinAI 6053 ACCGGT A^CCGGT
PshAI 433 GACGAAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 2908 AACGTT AA^CGTT
SacII 6 CCGCGG CCGC^GG
SpeI 4179 ACTAGT A^CTAGT
SphI 5628 GCATGC GCATG^C
SspBI 6588 TGTACA T^GTACA
SwaI 4860 ATTTAAAT ATTT^AAAT
XcmI 4673 CCACCAAGAATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 786 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccII 7 CGCG CG^CG
514 CGCG
AflIII 229 ACATGT A^C[GA][CT]GT
7484 ACATGT
Alw44I 1801 GTGCAC G^TGCAC
4464 GTGCAC
ApaLI 1801 GTGCAC G^TGCAC
4464 GTGCAC
AseI 326 ATTAAT AT^TAAT
7973 ATTAAT
AsnI 326 ATTAAT AT^TAAT
7973 ATTAAT
BbeI 13 GGCGCC GGCGC^C
20 GGCGCC
BsaI 82 GAGACC GGTCTC(1/5)
737 GGTCTC
BspLU11I 229 ACATGT A^CATGT
7484 ACATGT
BstUI 7 CGCG CG^CG
514 CGCG
BstXI 4676 CCAAGAATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
6465 CCAGATAGCTGG
EaeI 1201 TGGCCA [CT]^GGCC[GA]
1342 CGGCCA
HincII 5701 GTCAAC GT[CT]^[GA]AC
6057 GTCAAC
HindII 5701 GTCAAC GT[CT]^[GA]AC
6057 GTCAAC
KasI 13 GGCGCC G^GCGCC
20 GGCGCC
MvnI 7 CGCG CG^CG
514 CGCG
NarI 13 GGCGCC GG^CGCC
20 GGCGCC
PshBI 326 ATTAAT AT^TAAT
7973 ATTAAT
SbfI 2258 CCTGCAGG CCTGCA^GG
4065 CCTGCAGG
SfoI 13 GGCGCC GGC^GCC
20 GGCGCC
SmaI 31 CCCGGG CCC^GGG
3592 CCCGGG
Sse8387I 2258 CCTGCAGG CCTGCA^GG
4065 CCTGCAGG
XbaI 1577 TCTAGA T^CTAGA
4779 TCTAGA
XmaCI 31 CCCGGG C^CCGGG
3592 CCCGGG
XmaI 31 CCCGGG C^CCGGG
3592 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 138 enzymes) #
#########################################
AciI AcsI AcyI AfaI AflII
AluI AlwI AlwNI AocI ApoI
Asp700I AspHI AvaI AvaII BanI
BanII BbsI BbvI BclI BcnI
BfaI BfrI BglII BmyI BpmI
BpuAI BsaHI BsaJI BsaWI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BspHI
BspMI BsrBI BsrDI BsrFI BsrI
BssKI BstAPI BstEII BstF5I BstNI
BstPI BstYI Bsu36I Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraI
DraII DsaI EarI Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoRII EcoT14I
EcoT22I FauI FbaI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MfeI MflI
MnlI MseI MslI MspA1I MspI
MunI MvaI MwoI NciI NdeI
NdeII NlaIII NlaIV NsiI NspI
PflMI PleI PpuMI PstI PvuII
RcaI RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XhoII XmnI