##################################
# NO CUT ENZYMES ( 52 enzymes) #
##################################
AccIII AclI AgeI AhdI AseI
AsnI AspEI AviII BamHI BclI
BseAI BsiWI BsmBI BsmI BspDI
BspEI BstBI ClaI CpoI DrdI
Eam1105I EcoRI EcoRV EcoT22I FbaI
FseI FspI HpaI MfeI MluI
MroI MunI NotI NruI NsiI
NspV PacI PaeR7I PinAI PmeI
PshAI PshBI Psp1406I PvuI RsrII
SexAI SfuI SnaBI SpeI SspI
SwaI XhoI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
Acc65I 1205 GGTACC G^GTACC
AflII 907 CTTAAG C^TTAAG
AflIII 5251 ACATGT A^C[GA][CT]GT
Asp700I 5284 GAATGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1205 GGTACC G^GTACC
BbrPI 4299 CACGTG CAC^GTG
BciVI 1509 GTATCC GTATCC(6/5)
BfrI 907 CTTAAG C^TTAAG
BglII 774 AGATCT A^GATCT
BsaAI 4299 CACGTG [CT]AC^GT[GA]
BsaBI 1659 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BspHI 4828 TCATGA T^CATGA
BspLU11I 5251 ACATGT A^CATGT
BsrDI 662 GCAATG GCAATG(2/0)
Bst1107I 2029 GTATAC GTA^TAC
BstEII 2778 GGTGACC G^GT[ACGT]ACC
BstPI 2778 GGTGACC G^GT[ACGT]ACC
BstZ17I 2029 GTATAC GTA^TAC
DraI 4855 TTTAAA TTT^AAA
EcoO65I 2778 GGTGACC G^GT[ACGT]ACC
HindIII 5125 AAGCTT A^AGCTT
KpnI 1205 GGTACC GGTAC^C
MamI 1659 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NcoI 1355 CCATGG C^CATGG
NdeI 5146 CATATG CA^TATG
PmaCI 4299 CACGTG CAC^GTG
PmlI 4299 CACGTG CAC^GTG
RcaI 4828 TCATGA T^CATGA
SalI 1792 GTCGAC G^TCGAC
SbfI 4820 CCTGCAGG CCTGCA^GG
ScaI 4039 AGTACT AGT^ACT
SgrAI 1564 CGCCGGTG C[GA]^CCGG[CT]G
SphI 1035 GCATGC GCATG^C
Sse8387I 4820 CCTGCAGG CCTGCA^GG
TaqI 1793 TCGA T^CGA
TthHB8I 1793 TCGA T^CGA
XbaI 4314 TCTAGA T^CTAGA
XmnI 5284 GAATGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AatII 160 GACGTC GACGT^C
3649 GACGTC
AcsI 1777 AAATTT [GA]^AATT[CT]
5323 AAATTT
AocI 705 CCTGAGG CC^T[ACGT]AGG
1328 CCTCAGG
Aor51HI 332 AGCGCT AGC^GCT
2837 AGCGCT
ApoI 1777 AAATTT [GA]^AATT[CT]
5323 AAATTT
AscI 460 GGCGCGCC GG^CGCGCC
2951 GGCGCGCC
AspI 1302 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3646 GACGACGTC
AvrII 4177 CCTAGG C^CTAGG
4978 CCTAGG
BcgI 1428 CGAGCGTGTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1898 GCAGCCGCATCG
BlnI 4177 CCTAGG C^CTAGG
4978 CCTAGG
BsaI 811 GAGACC GGTCTC(1/5)
1402 GAGACC
Bsp1407I 1643 TGTACA T^GTACA
1709 TGTACA
BsrGI 1643 TGTACA T^GTACA
1709 TGTACA
BssSI 4209 CACGAG CACGAG(-5/-1)
2946 CTCGTG
BstAPI 184 GCAGGACATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2127 GCACATGCTGC
Bsu36I 705 CCTGAGG CC^T[ACGT]AGG
1328 CCTCAGG
DraIII 2020 CACCCCGTG CAC[ACGT][ACGT][ACGT]^GTG
4250 CACAAGGTG
Eco47III 332 AGCGCT AGC^GCT
2837 AGCGCT
Eco81I 705 CCTGAGG CC^T[ACGT]AGG
1328 CCTCAGG
HincII 1792 GTCGAC GT[CT]^[GA]AC
2425 GTCAAC
HindII 1792 GTCGAC GT[CT]^[GA]AC
2425 GTCAAC
NheI 1726 GCTAGC G^CTAGC
4216 GCTAGC
PflFI 1302 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3646 GACGACGTC
SacI 2299 GAGCTC GAGCT^C
4784 GAGCTC
SapI 3708 GAAGAGC GCTCTTC(1/4)
4668 GAAGAGC
SfiI 2802 GGCCATGTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
3511 GGCCGGGCAGGCC
SspBI 1643 TGTACA T^GTACA
1709 TGTACA
Tsp509I 1778 AATT ^AATT
5324 AATT
Tth111I 1302 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3646 GACGACGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 139 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AluI Alw44I AlwI AlwNI ApaI
ApaLI AspHI AvaI AvaII BalI
BanI BanII BbeI BbsI BbvCI
BbvI BcnI BfaI BglI BlpI
BmyI BpmI Bpu1102I BpuAI BsaHI
BsaJI BsaWI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrFI
BsrI BssHII BssKI BstF5I BstNI
BstUI BstXI BstYI Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EagI EarI
EclXI Eco52I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI Ksp632I KspI MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NarI NciI NdeII NgoMIV NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RsaI SacII Sau3AI
Sau96I ScrFI SfaNI SfcI SfoI
SmaI SmlI StuI StyI TfiI
Tru9I TseI Tsp45I TspRI Van91I
XcmI XhoII XmaCI XmaI