##################################
# NO CUT ENZYMES ( 101 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AgeI AhdI Aor51HI ApaI AscI
Asp700I Asp718I AspEI AvaI AviII
AvrII BamHI BbrPI BcgI BclI
BglI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaWI BseAI BsiWI
BsoBI Bsp1407I BspDI BspEI BsrBI
BsrFI BsrGI BssHII Bst1107I BstBI
BstEII BstPI BstUI BstZ17I CelII
Cfr10I ClaI CpoI DrdI Eam1105I
Eco47III EcoO65I EcoT22I FbaI FseI
FspI KpnI KspI MamI MluI
MroI MvnI NaeI NdeI NgoMIV
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshAI Psp1406I PvuI PvuII RsrII
SacII SalI SbfI ScaI SexAI
SfiI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI XcmI XhoI XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AccI 705 GTCTAC GT^[AC][GT]AC
AcyI 1060 GGCGCC G[GA]^CG[CT]C
AflII 2641 CTTAAG C^TTAAG
AseI 3635 ATTAAT AT^TAAT
AsnI 3635 ATTAAT AT^TAAT
BbeI 1060 GGCGCC GGCGC^C
BbsI 254 GTCTTC GAAGAC(2/6)
BciVI 3139 GTATCC GTATCC(6/5)
BcnI 4156 CCGGG CC^[GC]GG
BfrI 2641 CTTAAG C^TTAAG
BpuAI 254 GTCTTC GAAGAC(2/6)
BsaHI 1060 GGCGCC G[GA]^CG[CT]C
BsiEI 4153 CGGCCG CG[GA][CT]^CG
BsmBI 4363 CGTCTC CGTCTC(1/5)
BsmI 2597 GAATGC GAATGC(1/-1)
BspHI 1669 TCATGA T^CATGA
BssSI 3367 CACGAG CACGAG(-5/-1)
BstAPI 4299 GCAGAGGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 155 AGATCC [GA]^GATC[CT]
EagI 4153 CGGCCG C^GGCCG
EclXI 4153 CGGCCG C^GGCCG
Eco52I 4153 CGGCCG C^GGCCG
EcoRI 4010 GAATTC G^AATTC
EcoRV 3934 GATATC GAT^ATC
HaeII 1060 GGCGCC [GA]GCGC^[CT]
HgaI 4362 GCGTC GACGC(5/10)
Hin1I 1060 GGCGCC G[GA]^CG[CT]C
HincII 3573 GTTAAC GT[CT]^[GA]AC
HindII 3573 GTTAAC GT[CT]^[GA]AC
HpaI 3573 GTTAAC GTT^AAC
KasI 1060 GGCGCC G^GCGCC
MaeII 1376 ACGT A^CGT
MfeI 2746 CAATTG C^AATTG
MflI 155 AGATCC [GA]^GATC[CT]
MspA1I 3283 CCGCTG C[AC]G^C[GT]G
MunI 2746 CAATTG C^AATTG
NarI 1060 GGCGCC GG^CGCC
NciI 4156 CCGGG CC^[GC]GG
NheI 3101 GCTAGC G^CTAGC
PshBI 3635 ATTAAT AT^TAAT
RcaI 1669 TCATGA T^CATGA
SfoI 1060 GGCGCC GGC^GCC
XbaI 605 TCTAGA T^CTAGA
XhoII 155 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
Alw44I 241 GTGCAC G^TGCAC
652 GTGCAC
AocI 1410 CCTCAGG CC^T[ACGT]AGG
2922 CCTCAGG
ApaLI 241 GTGCAC G^TGCAC
652 GTGCAC
AspI 3667 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4358 GACTGCGTC
BalI 2119 TGGCCA TGG^CCA
4242 TGGCCA
BanI 1060 GGCGCC G^G[CT][GA]CC
2834 GGCACC
BbvCI 4199 GCTGAGG CCTCAGC(-5/-2)
4268 GCTGAGG
BseRI 360 GAGGAG GAGGAG(10/8)
4103 GAGGAG
BsrDI 3587 GCAATG GCAATG(2/0)
3952 GCAATG
Bsu36I 1410 CCTCAGG CC^T[ACGT]AGG
2922 CCTCAGG
CfoI 1061 GCGC GCG^C
4160 GCGC
DraI 663 TTTAAA TTT^AAA
1307 TTTAAA
DraIII 1206 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
3296 CACAGAGTG
Eco81I 1410 CCTCAGG CC^T[ACGT]AGG
2922 CCTCAGG
FauI 2541 CCCGC CCCGC(4/6)
3282 CCCGC
HhaI 1061 GCGC GCG^C
4160 GCGC
HinP1I 1061 GCGC G^CGC
4160 GCGC
HindIII 787 AAGCTT A^AGCTT
1283 AAGCTT
MluNI 2119 TGGCCA TGG^CCA
4242 TGGCCA
MscI 2119 TGGCCA TGG^CCA
4242 TGGCCA
PflFI 3667 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4358 GACTGCGTC
PflMI 559 CCAATTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3349 CCATTAGTTGG
SapI 2232 GAAGAGC GCTCTTC(1/4)
3113 GAAGAGC
Tth111I 3667 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4358 GACTGCGTC
Van91I 559 CCAATTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3349 CCATTAGTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 88 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI AspHI AvaII
BanII BbvI BfaI BmyI BpmI
BsaI BsaJI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BspLU11I
BspMI BsrI BssKI BstF5I BstNI
BstXI Cac8I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoNI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MnlI MseI MslI MspI
MvaI MwoI NcoI NdeII NlaIII
NlaIV NspI PleI PpuMI PstI
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I