##################################
# NO CUT ENZYMES ( 64 enzymes) #
##################################
AatII AccII AccIII AcyI AgeI
AscI AviII BbeI BbrPI BglI
BsaHI BsaWI BseAI BsiEI BsiWI
BsmBI BspDI BspEI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstUI
BstZ17I ClaI CpoI EagI EclXI
Eco52I EcoO65I FseI FspI Hin1I
KasI KspI MluI MroI MvnI
NarI NheI NotI NruI NspV
PacI PinAI PmaCI PmeI PmlI
PvuI RsrII SacII SbfI SfiI
SfoI SfuI SgrAI SmaI SnaBI
Sse8387I SwaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 26 enzymes) #
###################################
AclI 8540 AACGTT AA^CGTT
AflIII 5146 ACATGT A^C[GA][CT]GT
Aor51HI 5822 AGCGCT AGC^GCT
BlpI 2506 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2506 GCTCAGC GC^T[ACGT]AGC
BsaAI 5495 TACGTG [CT]AC^GT[GA]
BspLU11I 5146 ACATGT A^CATGT
BsrBI 2092 GAGCGG CCGCTC(-3/-3)
BsrFI 7164 GCCGGC [GA]^CCGG[CT]
CelII 2506 GCTCAGC GC^T[ACGT]AGC
Cfr10I 7164 GCCGGC [GA]^CCGG[CT]
Eco47III 5822 AGCGCT AGC^GCT
EcoRV 6598 GATATC GAT^ATC
EcoT22I 1663 ATGCAT ATGCA^T
HaeII 5822 AGCGCT [GA]GCGC^[CT]
NaeI 7164 GCCGGC GCC^GGC
NgoMIV 7164 GCCGGC G^CCGGC
NsiI 1663 ATGCAT ATGCA^T
PaeR7I 1022 CTCGAG C^TCGAG
Psp1406I 8540 AACGTT AA^CGTT
SacI 2504 GAGCTC GAGCT^C
SalI 5540 GTCGAC G^TCGAC
SapI 88 GAAGAGC GCTCTTC(1/4)
ScaI 6810 AGTACT AGT^ACT
SpeI 9762 ACTAGT A^CTAGT
XhoI 1022 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AflII 3244 CTTAAG C^TTAAG
6579 CTTAAG
AhdI 3214 GACACAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
9304 GACCAAGAGTC
Alw44I 1353 GTGCAC G^TGCAC
6196 GTGCAC
AlwNI 1506 CAGACACTG CAG[ACGT][ACGT][ACGT]^CTG
5885 CAGTATCTG
ApaI 6135 GGGCCC GGGCC^C
9626 GGGCCC
ApaLI 1353 GTGCAC G^TGCAC
6196 GTGCAC
AspEI 3214 GACACAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
9304 GACCAAGAGTC
AvaI 1022 CTCGAG C^[CT]CG[GA]G
8477 CTCGGG
BcgI 1030 GCAGGACTGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2199 GCAAAGAAGTCG
BfrI 3244 CTTAAG C^TTAAG
6579 CTTAAG
BsoBI 1022 CTCGAG C^[CT]CG[GA]G
8477 CTCGGG
BspHI 3017 TCATGA T^CATGA
6269 TCATGA
CfoI 1568 GCGC GCG^C
5823 GCGC
DrdI 2824 GACAAAATGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
8670 GACATCTGTGTC
Eam1105I 3214 GACACAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
9304 GACCAAGAGTC
EcoNI 5578 CCTCATCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
5680 CCTCCTTTAGG
FauI 5569 CCCGC CCCGC(4/6)
2094 GCGGG
HgaI 1759 GCGTC GACGC(5/10)
3606 GCGTC
HhaI 1568 GCGC GCG^C
5823 GCGC
HinP1I 1568 GCGC G^CGC
5823 GCGC
HpaI 5617 GTTAAC GTT^AAC
7333 GTTAAC
PshAI 851 GACCTCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
7254 GACAGTAGTC
RcaI 3017 TCATGA T^CATGA
6269 TCATGA
SexAI 2172 ACCAGGT A^CC[AT]GGT
5175 ACCTGGT
SphI 1665 GCATGC GCATG^C
7685 GCATGC
SspI 3307 AATATT AAT^ATT
7591 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 142 enzymes) #
#########################################
Acc65I AccI AciI AcsI AfaI
AluI AlwI AocI ApoI AseI
AsnI Asp700I Asp718I AspHI AspI
AvaII AvrII BalI BamHI BanI
BanII BbsI BbvCI BbvI BciVI
BclI BcnI BfaI BglII BlnI
BmyI BpmI BpuAI BsaBI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
Bsp1407I BspMI BsrDI BsrGI BsrI
BssKI BstAPI BstF5I BstNI BstXI
BstYI Bsu36I Cac8I Cfr13I DdeI
DpnII DraI DraII DraIII DsaI
EaeI EarI Eco81I EcoO109I EcoRI
EcoRII EcoT14I FbaI Fnu4HI FokI
HaeIII HapII HincII HindII HindIII
HinfI HpaII HphI ItaI KpnI
Ksp632I MaeI MaeII MaeIII MamI
MboI MboII MfeI MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MunI MvaI MwoI NciI
NcoI NdeI NdeII NlaIII NlaIV
NspI PflFI PflMI PleI PpuMI
PshBI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspBI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Tth111I TthHB8I Van91I XbaI XcmI
XhoII XmnI