##################################
# NO CUT ENZYMES (   88 enzymes) #
##################################

Acc65I      AccI        AclI        AflII       AgeI        
Alw44I      ApaLI       AscI        AseI        AsnI        
Asp700I     Asp718I     AspHI       AspI        AviII       
AvrII       BamHI       BcgI        BciVI       BfaI        
BfrI        BglII       BlnI        BsaBI       BsgI        
BsiHKAI     BsmBI       BsmI        Bsp1407I    BspDI       
BspHI       BspLU11I    BsrDI       BsrGI       Bst1107I    
BstAPI      BstBI       BstZ17I     ClaI        DraIII      
DrdI        EcoNI       EcoRI       EcoT22I     FseI        
FspI        HpaI        KpnI        MaeI        MamI        
MfeI        MluI        MunI        NcoI        NdeI        
NheI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PmeI        
PshAI       PshBI       Psp1406I    PvuI        RcaI        
SacI        SalI        SapI        ScaI        SexAI       
SfuI        SgrAI       SnaBI       SpeI        SspBI       
SspI        SwaI        Tth111I     Van91I      XbaI        
XcmI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   47 enzymes) #
###################################

AatII        1971  GACGTC               GACGT^C
AccIII        725  TCCGGA               T^CCGGA
AcsI         1870  AAATTC               [GA]^AATT[CT]
AflIII       1094  ACGTGT               A^C[GA][CT]GT
AhdI         2176  GACCCGGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI         1870  AAATTC               [GA]^AATT[CT]
AspEI        2176  GACCCGGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbsI          391  GAAGAC               GAAGAC(2/6)
BbvCI        1141  GCTGAGG              CCTCAGC(-5/-2)
BlpI          957  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      957  GCTGAGC              GC^T[ACGT]AGC
BpuAI         391  GAAGAC               GAAGAC(2/6)
BseAI         725  TCCGGA               T^CCGGA
BsiWI        2269  CGTACG               C^GTACG
BspEI         725  TCCGGA               T^CCGGA
BssHII        943  GCGCGC               G^CGCGC
BstEII       1998  GGTGACC              G^GT[ACGT]ACC
BstPI        1998  GGTGACC              G^GT[ACGT]ACC
BstXI        1272  CCATAGCTCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI        1839  GGATCT               [GA]^GATC[CT]
CelII         957  GCTGAGC              GC^T[ACGT]AGC
CpoI           58  CGGACCG              CG^G[AT]CCG
DraI         2204  TTTAAA               TTT^AAA
Eam1105I     2176  GACCCGGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI         2071  GAAGAG               CTCTTC(1/4)
EcoO65I      1998  GGTGACC              G^GT[ACGT]ACC
EcoRV         835  GATATC               GAT^ATC
HincII       2310  GTCAAC               GT[CT]^[GA]AC
HindII       2310  GTCAAC               GT[CT]^[GA]AC
HindIII      1246  AAGCTT               A^AGCTT
Ksp632I      2071  GAAGAG               CTCTTC(1/4)
KspI         1647  CCGCGG               CCGC^GG
MflI         1839  GGATCT               [GA]^GATC[CT]
MroI          725  TCCGGA               T^CCGGA
MslI         1852  CACTACTATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NotI         2429  GCGGCCGC             GC^GGCCGC
PvuII         277  CAGCTG               CAG^CTG
RsrII          58  CGGACCG              CG^G[AT]CCG
SacII        1647  CCGCGG               CCGC^GG
SbfI         1668  CCTGCAGG             CCTGCA^GG
SphI         1546  GCATGC               GCATG^C
Sse8387I     1668  CCTGCAGG             CCTGCA^GG
StuI          155  AGGCCT               AGG^CCT
TaqI         1778  TCGA                 T^CGA
Tsp509I      1871  AATT                 ^AATT
TthHB8I      1778  TCGA                 T^CGA
XhoII        1839  GGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AlwNI         500  CAGCCCCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1378  CAGGACCTG           
Aor51HI       629  AGCGCT               AGC^GCT
             1679  AGCGCT              
BalI          380  TGGCCA               TGG^CCA
              614  TGGCCA              
BbeI          689  GGCGCC               GGCGC^C
             1032  GGCGCC              
BbrPI        1093  CACGTG               CAC^GTG
             1361  CACGTG              
BclI         1235  TGATCA               T^GATCA
             1700  TGATCA              
BsaAI        1093  CACGTG               [CT]AC^GT[GA]
             1361  CACGTG              
BsaI         1078  GAGACC               GGTCTC(1/5)
              480  GGTCTC              
BsaWI          29  ACCGGA               [AT]^CCGG[AT]
              725  TCCGGA              
BssSI        1568  CACGAG               CACGAG(-5/-1)
             1057  CTCGTG              
Eco47III      629  AGCGCT               AGC^GCT
             1679  AGCGCT              
FbaI         1235  TGATCA               T^GATCA
             1700  TGATCA              
KasI          689  GGCGCC               G^GCGCC
             1032  GGCGCC              
MluNI         380  TGGCCA               TGG^CCA
              614  TGGCCA              
MscI          380  TGGCCA               TGG^CCA
              614  TGGCCA              
NaeI          984  GCCGGC               GCC^GGC
             1009  GCCGGC              
NarI          689  GGCGCC               GG^CGCC
             1032  GGCGCC              
NgoMIV        984  GCCGGC               G^CCGGC
             1009  GCCGGC              
PmaCI        1093  CACGTG               CAC^GTG
             1361  CACGTG              
PmlI         1093  CACGTG               CAC^GTG
             1361  CACGTG              
SfiI          372  GGCCCCTTTGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
             1318  GGCCTGGGGGGCC       
SfoI          689  GGCGCC               GGC^GCC
             1032  GGCGCC              


#########################################
# 3 OR MORE CUT ENZYMES (  101 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwI        AocI        ApaI        AvaI        AvaII       
BanI        BanII       BbvI        BcnI        BglI        
BmyI        BpmI        BsaHI       BsaJI       BseRI       
BsiEI       BsiYI       BslI        BsmAI       BsmFI       
BsoBI       Bsp1286I    BspMI       BsrBI       BsrFI       
BsrI        BssKI       BstF5I      BstNI       BstUI       
Bsu36I      Cac8I       CfoI        Cfr10I      Cfr13I      
DdeI        DpnII       DraII       DsaI        EaeI        
EagI        EclXI       Eco52I      Eco81I      EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HgaI        HhaI        
Hin1I       HinP1I      HinfI       HpaII       HphI        
ItaI        MaeII       MaeIII      MboI        MboII       
MnlI        MseI        MspA1I      MspI        MvaI        
MvnI        MwoI        NciI        NdeII       NlaIII      
NlaIV       NspI        PleI        PpuMI       PstI        
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SmaI        SmlI        StyI        TfiI        
Tru9I       TseI        Tsp45I      TspRI       XmaCI       
XmaI