##################################
# NO CUT ENZYMES ( 100 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
AhdI Aor51HI AscI AseI AsnI
Asp718I AspEI AspI AvaI AviII
AvrII BamHI BbrPI BcgI BcnI
BlnI BlpI Bpu1102I BsaWI BseAI
BsiEI BsiWI BsoBI BspDI BspEI
BsrBI BsrDI BsrFI BssHII BssSI
BstBI CelII Cfr10I ClaI CpoI
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoRI EcoRV FauI FseI
FspI HapII HincII HindII HindIII
HpaI HpaII KpnI KspI MroI
MspI NaeI NciI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI RsrII SacII SalI SapI
SbfI ScaI SexAI SfiI SfuI
SgrAI SmaI SpeI SphI Sse8387I
SwaI Tth111I XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AcyI 4373 GGCGCC G[GA]^CG[CT]C
AflII 1319 CTTAAG C^TTAAG
AocI 4461 CCTCAGG CC^T[ACGT]AGG
BalI 3303 TGGCCA TGG^CCA
BbeI 4373 GGCGCC GGCGC^C
BclI 73 TGATCA T^GATCA
BfrI 1319 CTTAAG C^TTAAG
BsaAI 4645 TACGTA [CT]AC^GT[GA]
BsaHI 4373 GGCGCC G[GA]^CG[CT]C
BsaI 4445 GGTCTC GGTCTC(1/5)
BsmBI 2744 CGTCTC CGTCTC(1/5)
BspHI 4148 TCATGA T^CATGA
BstEII 2100 GGTGACC G^GT[ACGT]ACC
BstPI 2100 GGTGACC G^GT[ACGT]ACC
BstXI 4489 CCAAAGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 4461 CCTCAGG CC^T[ACGT]AGG
DraI 3205 TTTAAA TTT^AAA
DraIII 2272 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 3303 TGGCCA [CT]^GGCC[GA]
Eco81I 4461 CCTCAGG CC^T[ACGT]AGG
EcoO65I 2100 GGTGACC G^GT[ACGT]ACC
EcoT22I 2525 ATGCAT ATGCA^T
FbaI 73 TGATCA T^GATCA
HaeII 4373 GGCGCC [GA]GCGC^[CT]
HgaI 4685 GCGTC GACGC(5/10)
Hin1I 4373 GGCGCC G[GA]^CG[CT]C
KasI 4373 GGCGCC G^GCGCC
MluI 4683 ACGCGT A^CGCGT
MluNI 3303 TGGCCA TGG^CCA
MscI 3303 TGGCCA TGG^CCA
NarI 4373 GGCGCC GG^CGCC
NcoI 959 CCATGG C^CATGG
NdeI 2255 CATATG CA^TATG
NsiI 2525 ATGCAT ATGCA^T
PflMI 1083 CCAAGAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 4148 TCATGA T^CATGA
SacI 3716 GAGCTC GAGCT^C
SfoI 4373 GGCGCC GGC^GCC
SnaBI 4645 TACGTA TAC^GTA
Van91I 1083 CCAAGAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccI 2250 GTATAC GT^[AC][GT]AC
3570 GTATAC
AccII 4291 CGCG CG^CG
4684 CGCG
Alw44I 2278 GTGCAC G^TGCAC
4543 GTGCAC
AlwNI 629 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
2720 CAGCTGCTG
ApaI 238 GGGCCC GGGCC^C
2929 GGGCCC
ApaLI 2278 GTGCAC G^TGCAC
4543 GTGCAC
AvaII 1010 GGACC G^G[AT]CC
2360 GGTCC
BbvCI 1777 CCTCAGC CCTCAGC(-5/-2)
262 GCTGAGG
BglI 2710 GCCATACAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3673 GCCTAGAGGGC
BsaBI 424 GATGGGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2887 GATGGAGATC
BseRI 1534 CTCCTC GAGGAG(10/8)
2737 CTCCTC
BsmI 481 GAATGC GAATGC(1/-1)
3257 GAATGC
Bsp1407I 2050 TGTACA T^GTACA
2775 TGTACA
BspLU11I 2246 ACATGT A^CATGT
2282 ACATGT
BsrGI 2050 TGTACA T^GTACA
2775 TGTACA
Bst1107I 2250 GTATAC GTA^TAC
3570 GTATAC
BstUI 4291 CGCG CG^CG
4684 CGCG
BstZ17I 2250 GTATAC GTA^TAC
3570 GTATAC
DsaI 959 CCATGG C^C[GA][CT]GG
989 CCGTGG
EarI 1555 CTCTTC CTCTTC(1/4)
2498 CTCTTC
EcoNI 2694 CCTCTAATAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3300 CCTTGGCCAGG
Ksp632I 1555 CTCTTC CTCTTC(1/4)
2498 CTCTTC
MamI 424 GATGGGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2887 GATGGAGATC
MfeI 172 CAATTG C^AATTG
1112 CAATTG
MspA1I 2720 CAGCTG C[AC]G^C[GT]G
3881 CAGCTG
MunI 172 CAATTG C^AATTG
1112 CAATTG
MvnI 4291 CGCG CG^CG
4684 CGCG
PvuII 2720 CAGCTG CAG^CTG
3881 CAGCTG
SspBI 2050 TGTACA T^GTACA
2775 TGTACA
SspI 1503 AATATT AAT^ATT
1993 AATATT
StuI 3308 AGGCCT AGG^CCT
3385 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 87 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI ApoI Asp700I AspHI BanI
BanII BbsI BbvI BciVI BfaI
BglII BmyI BpmI BpuAI BsaJI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BspMI BsrI BssKI
BstAPI BstF5I BstNI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeIII HhaI HinP1I HinfI HphI
ItaI MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MvaI MwoI NdeII NlaIII NlaIV
NspI PleI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XbaI XcmI
XhoII XmnI