##################################
# NO CUT ENZYMES (   77 enzymes) #
##################################

AatII       AccIII      AclI        AgeI        AscI        
AseI        AsnI        Asp700I     AviII       BamHI       
BbeI        BsaBI       BsaWI       BseAI       BsiEI       
BsiWI       Bsp1407I    BspDI       BspEI       BspHI       
BsrGI       BssHII      Bst1107I    BstBI       BstZ17I     
ClaI        CpoI        EagI        EclXI       Eco52I      
EcoRV       EcoT22I     FseI        FspI        HindIII     
HpaI        KasI        MamI        MfeI        MluI        
MroI        MunI        NarI        NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PinAI       PmeI        PshAI       PshBI       Psp1406I    
PvuI        RcaI        RsrII       SalI        SapI        
SbfI        ScaI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        SwaI        XbaI        
XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   43 enzymes) #
###################################

Acc65I       4240  GGTACC               G^GTACC
AccI         3342  GTCTAC               GT^[AC][GT]AC
AflII        4800  CTTAAG               C^TTAAG
AflIII       3178  ACATGT               A^C[GA][CT]GT
AhdI         4913  GACTGCCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI         4965  CCTCAGG              CC^T[ACGT]AGG
Aor51HI       949  AGCGCT               AGC^GCT
Asp718I      4240  GGTACC               G^GTACC
AspEI        4913  GACTGCCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI         1968  GACAGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI        2070  CACGTG               CAC^GTG
BbvCI        2453  CCTCAGC              CCTCAGC(-5/-2)
BcgI         1174  GCAATGGCGTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI         131  GTATCC               GTATCC(6/5)
BclI         3833  TGATCA               T^GATCA
BfrI         4800  CTTAAG               C^TTAAG
BglII        2939  AGATCT               A^GATCT
BsaAI        2070  CACGTG               [CT]AC^GT[GA]
BsmBI         502  GAGACG               CGTCTC(1/5)
BsmI          446  GCATTC               GAATGC(1/-1)
BspLU11I     3178  ACATGT               A^CATGT
BsrBI        2043  CCGCTC               CCGCTC(-3/-3)
BstEII        426  GGTTACC              G^GT[ACGT]ACC
BstPI         426  GGTTACC              G^GT[ACGT]ACC
Bsu36I       4965  CCTCAGG              CC^T[ACGT]AGG
Eam1105I     4913  GACTGCCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III      949  AGCGCT               AGC^GCT
Eco81I       4965  CCTCAGG              CC^T[ACGT]AGG
EcoO65I       426  GGTTACC              G^GT[ACGT]ACC
EcoRI        3653  GAATTC               G^AATTC
FbaI         3833  TGATCA               T^GATCA
KpnI         4240  GGTACC               GGTAC^C
KspI          136  CCGCGG               CCGC^GG
NdeI         3378  CATATG               CA^TATG
PflFI        1968  GACAGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI        2070  CACGTG               CAC^GTG
PmlI         2070  CACGTG               CAC^GTG
SacI         1058  GAGCTC               GAGCT^C
SacII         136  CCGCGG               CCGC^GG
SmaI           26  CCCGGG               CCC^GGG
Tth111I      1968  GACAGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI          26  CCCGGG               C^CCGGG
XmaI           26  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

Alw44I       3404  GTGCAC               G^TGCAC
             5039  GTGCAC              
ApaLI        3404  GTGCAC               G^TGCAC
             5039  GTGCAC              
BbsI         2881  GAAGAC               GAAGAC(2/6)
             4574  GAAGAC              
BglI          919  GCCGTGTGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             1666  GCCCAGTTGGC         
BlpI          107  GCTGAGC              GC^T[ACGT]AGC
             2221  GCTGAGC             
Bpu1102I      107  GCTGAGC              GC^T[ACGT]AGC
             2221  GCTGAGC             
BpuAI        2881  GAAGAC               GAAGAC(2/6)
             4574  GAAGAC              
BsrDI        3547  CATTGC               GCAATG(2/0)
             1174  GCAATG              
BsrFI         112  GCCGGC               [GA]^CCGG[CT]
             1006  GCCGGC              
BssSI        4752  CACGAG               CACGAG(-5/-1)
              798  CTCGTG              
BstAPI        764  GCAGGCAGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             1939  GCAACTGGTGC         
CelII         107  GCTGAGC              GC^T[ACGT]AGC
             2221  GCTGAGC             
Cfr10I        112  GCCGGC               [GA]^CCGG[CT]
             1006  GCCGGC              
DraI         2167  TTTAAA               TTT^AAA
             3827  TTTAAA              
DrdI         1038  GACAGTGAGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1460  GACGGATGAGTC        
MaeII         505  ACGT                 A^CGT
             2071  ACGT                
NaeI          112  GCCGGC               GCC^GGC
             1006  GCCGGC              
NgoMIV        112  GCCGGC               G^CCGGC
             1006  GCCGGC              
PpuMI        1450  AGGACCT              [GA]G^G[AT]CC[CT]
             2688  GGGTCCT             
TaqI          839  TCGA                 T^CGA
              937  TCGA                
TthHB8I       839  TCGA                 T^CGA
              937  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (  117 enzymes) #
#########################################

AccII       AciI        AcsI        AcyI        AfaI        
AluI        AlwI        AlwNI       ApaI        ApoI        
AspHI       AvaI        AvaII       AvrII       BalI        
BanI        BanII       BbvI        BcnI        BfaI        
BlnI        BmyI        BpmI        BsaHI       BsaI        
BsaJI       BseRI       BsgI        BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       BsoBI       Bsp1286I    
BspMI       BsrI        BssKI       BstF5I      BstNI       
BstUI       BstXI       BstYI       Cac8I       CfoI        
Cfr13I      DdeI        DpnII       DraII       DraIII      
DsaI        EaeI        EarI        EcoNI       EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HgaI        HhaI        
Hin1I       HinP1I      HincII      HindII      HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeI        
MaeIII      MboI        MboII       MflI        MluNI       
MnlI        MscI        MseI        MslI        MspA1I      
MspI        MvaI        MvnI        MwoI        NciI        
NcoI        NdeII       NlaIII      NlaIV       NspI        
PflMI       PleI        PstI        PvuII       RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmlI        StuI        StyI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       Van91I      
XcmI        XhoII