##################################
# NO CUT ENZYMES ( 68 enzymes) #
##################################
AatII Acc65I AccI AccIII AflII
AflIII AhdI AocI Aor51HI AscI
Asp718I AspEI AvrII BamHI BciVI
BclI BfrI BglII BlnI BsaBI
BseAI BsiWI BsmI BspDI BspEI
BspLU11I Bst1107I BstBI BstYI BstZ17I
Bsu36I ClaI CpoI DraI Eam1105I
Eco47III Eco81I EcoT22I FbaI FseI
HincII HindII HindIII HpaI KpnI
MamI MfeI MflI MluI MroI
MunI NheI NruI NsiI NspV
PmeI PvuI RsrII SalI SfiI
SfuI SgrAI SnaBI SpeI SspI
SwaI XbaI XhoII
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AclI 1892 AACGTT AA^CGTT
AcsI 3442 GAATTC [GA]^AATT[CT]
AgeI 1444 ACCGGT A^CCGGT
ApoI 3442 GAATTC [GA]^AATT[CT]
AseI 4533 ATTAAT AT^TAAT
AsnI 4533 ATTAAT AT^TAAT
Asp700I 735 GAAGGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 1789 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 3266 TGGCCA TGG^CCA
BbeI 1721 GGCGCC GGCGC^C
BbrPI 1705 CACGTG CAC^GTG
BcgI 1898 GCAGCACACTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI 2918 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2918 GCTCAGC GC^T[ACGT]AGC
BsaWI 1444 ACCGGT [AT]^CCGG[AT]
Bsp1407I 3518 TGTACA T^GTACA
BspHI 1169 TCATGA T^CATGA
BsrDI 3500 CATTGC GCAATG(2/0)
BsrGI 3518 TGTACA T^GTACA
BssSI 1573 CTCGTG CACGAG(-5/-1)
BstEII 1997 GGTCACC G^GT[ACGT]ACC
BstPI 1997 GGTCACC G^GT[ACGT]ACC
BstXI 3046 CCAGAAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 2918 GCTCAGC GC^T[ACGT]AGC
EcoO65I 1997 GGTCACC G^GT[ACGT]ACC
EcoRI 3442 GAATTC G^AATTC
EcoRV 4546 GATATC GAT^ATC
KasI 1721 GGCGCC G^GCGCC
MluNI 3266 TGGCCA TGG^CCA
MscI 3266 TGGCCA TGG^CCA
NarI 1721 GGCGCC GG^CGCC
NdeI 3806 CATATG CA^TATG
NotI 1 GCGGCCGC GC^GGCCGC
PacI 4534 TTAATTAA TTAAT^TAA
PaeR7I 1906 CTCGAG C^TCGAG
PflFI 1789 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 1444 ACCGGT A^CCGGT
PmaCI 1705 CACGTG CAC^GTG
PmlI 1705 CACGTG CAC^GTG
PshAI 2992 GACATCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 4533 ATTAAT AT^TAAT
Psp1406I 1892 AACGTT AA^CGTT
RcaI 1169 TCATGA T^CATGA
SapI 1770 GCTCTTC GCTCTTC(1/4)
SbfI 1476 CCTGCAGG CCTGCA^GG
ScaI 4219 AGTACT AGT^ACT
SexAI 1583 ACCTGGT A^CC[AT]GGT
SfoI 1721 GGCGCC GGC^GCC
SmlI 1906 CTCGAG C^T[CT][GA]AG
Sse8387I 1476 CCTGCAGG CCTGCA^GG
SspBI 3518 TGTACA T^GTACA
Tth111I 1789 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 1906 CTCGAG C^TCGAG
XmnI 735 GAAGGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 11 enzymes) #
###################################
ApaI 1853 GGGCCC GGGCC^C
2730 GGGCCC
BbvCI 798 CCTCAGC CCTCAGC(-5/-2)
4279 CCTCAGC
BsaAI 706 TACGTG [CT]AC^GT[GA]
1705 CACGTG
BssHII 1766 GCGCGC G^CGCGC
2561 GCGCGC
DrdI 2154 GACCCCGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2901 GACCTCATGGTC
KspI 79 CCGCGG CCGC^GG
2627 CCGCGG
NaeI 727 GCCGGC GCC^GGC
3110 GCCGGC
NgoMIV 727 GCCGGC G^CCGGC
3110 GCCGGC
NspI 1843 GCATGC [GA]CATG^[CT]
2375 GCATGC
SacII 79 CCGCGG CCGC^GG
2627 CCGCGG
SphI 1843 GCATGC GCATG^C
2375 GCATGC
#########################################
# 3 OR MORE CUT ENZYMES ( 125 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
Alw44I AlwI AlwNI ApaLI AspHI
AvaI AvaII AviII BanI BanII
BbsI BbvI BcnI BfaI BglI
BmyI BpmI BpuAI BsaHI BsaI
BsaJI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrFI
BsrI BssKI BstAPI BstF5I BstNI
BstUI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DraIII DsaI
EaeI EagI EarI EclXI Eco52I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI FspI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV PflMI PleI
PpuMI PstI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XmaCI XmaI