##################################
# NO CUT ENZYMES ( 89 enzymes) #
##################################
AatII Acc65I AccII AclI AcyI
AgeI AhdI Alw44I AocI ApaI
ApaLI AscI Asp718I AspEI AvaI
AviII BamHI BbeI BbvCI BcgI
BclI BglII BsaHI BsaI BsiEI
BsiWI BsmI BsoBI Bsp1407I BspDI
BspHI BspLU11I BspMI BsrGI BssHII
BstBI BstEII BstPI BstUI Bsu36I
ClaI CpoI DraIII EagI Eam1105I
EclXI Eco52I Eco81I EcoO65I EcoRV
FbaI FseI FspI Hin1I KasI
KpnI KspI MluI MvnI NaeI
NarI NgoMIV NotI NruI NspV
PacI PaeR7I PinAI PmeI Psp1406I
PvuI RcaI RsrII SacII SalI
SbfI SexAI SfoI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SspBI
SwaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AccI 2929 GTATAC GT^[AC][GT]AC
AccIII 3277 TCCGGA T^CCGGA
AflII 419 CTTAAG C^TTAAG
AflIII 3299 ACACGT A^C[GA][CT]GT
AspI 5248 GACTAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 3300 CACGTG CAC^GTG
BciVI 544 GGATAC GTATCC(6/5)
BfrI 419 CTTAAG C^TTAAG
BsaBI 5137 GATTCTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 3277 TCCGGA T^CCGGA
BsmBI 3174 GAGACG CGTCTC(1/5)
BspEI 3277 TCCGGA T^CCGGA
BsrBI 13 GAGCGG CCGCTC(-3/-3)
BsrFI 1462 GCCGGT [GA]^CCGG[CT]
Bst1107I 2929 GTATAC GTA^TAC
BstAPI 3233 GCAACTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 2681 GGATCT [GA]^GATC[CT]
BstZ17I 2929 GTATAC GTA^TAC
Cfr10I 1462 GCCGGT [GA]^CCGG[CT]
DrdI 2153 GACTTCTGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 1820 CCTACTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HgaI 27 GCGTC GACGC(5/10)
HpaI 3710 GTTAAC GTT^AAC
MamI 5137 GATTCTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1037 CAATTG C^AATTG
MflI 2681 GGATCT [GA]^GATC[CT]
MroI 3277 TCCGGA T^CCGGA
MunI 1037 CAATTG C^AATTG
NcoI 1138 CCATGG C^CATGG
NdeI 1203 CATATG CA^TATG
NheI 1496 GCTAGC G^CTAGC
PflFI 5248 GACTAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 3300 CACGTG CAC^GTG
PmlI 3300 CACGTG CAC^GTG
SacI 1402 GAGCTC GAGCT^C
SfiI 3360 GGCCAGGATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
StuI 5380 AGGCCT AGG^CCT
Tth111I 5248 GACTAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 4040 TCTAGA T^CTAGA
XhoII 2681 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AlwNI 1071 CAGCAACTG CAG[ACGT][ACGT][ACGT]^CTG
2020 CAGCAGCTG
Aor51HI 684 AGCGCT AGC^GCT
2502 AGCGCT
AseI 3991 ATTAAT AT^TAAT
4891 ATTAAT
AsnI 3991 ATTAAT AT^TAAT
4891 ATTAAT
AspHI 1402 GAGCTC G[AT]GC[AT]^C
1694 GTGCTC
AvrII 2095 CCTAGG C^CTAGG
2379 CCTAGG
BanI 492 GGCACC G^G[CT][GA]CC
2553 GGTGCC
BbsI 2009 GAAGAC GAAGAC(2/6)
2890 GTCTTC
BglI 263 GCCTTAAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3361 GCCAGGATGGC
BlnI 2095 CCTAGG C^CTAGG
2379 CCTAGG
BlpI 778 GCTGAGC GC^T[ACGT]AGC
3269 GCTCAGC
Bpu1102I 778 GCTGAGC GC^T[ACGT]AGC
3269 GCTCAGC
BpuAI 2009 GAAGAC GAAGAC(2/6)
2890 GTCTTC
BsaAI 2922 TACGTG [CT]AC^GT[GA]
3300 CACGTG
BsaWI 945 ACCGGA [AT]^CCGG[AT]
3277 TCCGGA
BsiHKAI 1402 GAGCTC G[AT]GC[AT]^C
1694 GTGCTC
BssSI 2791 CACGAG CACGAG(-5/-1)
3386 CTCGTG
CelII 778 GCTGAGC GC^T[ACGT]AGC
3269 GCTCAGC
DsaI 1138 CCATGG C^C[GA][CT]GG
1292 CCACGG
Eco47III 684 AGCGCT AGC^GCT
2502 AGCGCT
EcoRI 2451 GAATTC G^AATTC
3576 GAATTC
EcoT22I 2832 ATGCAT ATGCA^T
4301 ATGCAT
FauI 690 CCCGC CCCGC(4/6)
3397 CCCGC
HaeII 684 AGCGCT [GA]GCGC^[CT]
2502 AGCGCT
NsiI 2832 ATGCAT ATGCA^T
4301 ATGCAT
PflMI 2486 CCAAAGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4935 CCAATCTCTGG
PshAI 1363 GACTCCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3176 GACGGGAGTC
PshBI 3991 ATTAAT AT^TAAT
4891 ATTAAT
ScaI 4371 AGTACT AGT^ACT
4389 AGTACT
SphI 459 GCATGC GCATG^C
535 GCATGC
SspI 2387 AATATT AAT^ATT
4119 AATATT
Van91I 2486 CCAAAGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4935 CCAATCTCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 97 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI Asp700I AvaII BalI BanII
BbvI BcnI BfaI BmyI BpmI
BsaJI BseRI BsgI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrDI BsrI
BssKI BstF5I BstNI BstXI Cac8I
CfoI Cfr13I DdeI DpnII DraI
DraII EaeI EarI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HhaI HinP1I HincII HindII HindIII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PpuMI PstI PvuII RsaI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI XmnI