##################################
# NO CUT ENZYMES (   74 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AflII       
AhdI        Alw44I      AocI        Aor51HI     ApaLI       
AscI        Asp718I     AspEI       AviII       AvrII       
BbrPI       BfrI        BglI        BlnI        BsaAI       
BsaBI       BseAI       BsiWI       BsmBI       BspDI       
BspEI       BssHII      BssSI       Bst1107I    BstZ17I     
Bsu36I      ClaI        CpoI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoRV       
FseI        FspI        HindIII     HpaI        KpnI        
MamI        MfeI        MluI        MroI        MunI        
NheI        NotI        NruI        PaeR7I      PflMI       
PmaCI       PmeI        PmlI        PshAI       Psp1406I    
PvuI        RsrII       SalI        SapI        SbfI        
SexAI       SfiI        SgrAI       SnaBI       SphI        
Sse8387I    SwaI        Van91I      XhoI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

AccI          421  GTCTAC               GT^[AC][GT]AC
AcyI          143  GGCGCC               G[GA]^CG[CT]C
AflIII       2455  ACATGT               A^C[GA][CT]GT
AgeI         2383  ACCGGT               A^CCGGT
AspI         2432  GACTTAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BamHI          28  GGATCC               G^GATCC
BbeI          143  GGCGCC               GGCGC^C
BbvCI        2131  CCTCAGC              CCTCAGC(-5/-2)
BcgI         1114  GCACCAGGCTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI         1188  TGATCA               T^GATCA
BlpI         1154  GCTAAGC              GC^T[ACGT]AGC
Bpu1102I     1154  GCTAAGC              GC^T[ACGT]AGC
BsaHI         143  GGCGCC               G[GA]^CG[CT]C
BsaWI        2383  ACCGGT               [AT]^CCGG[AT]
BsiEI         850  CGACCG               CG[GA][CT]^CG
Bsp1407I     3430  TGTACA               T^GTACA
BspLU11I     2455  ACATGT               A^CATGT
BsrGI        3430  TGTACA               T^GTACA
BstAPI       1375  GCACCAGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI        4561  TTCGAA               TT^CGAA
CelII        1154  GCTAAGC              GC^T[ACGT]AGC
EcoRI        2567  GAATTC               G^AATTC
FbaI         1188  TGATCA               T^GATCA
HaeII         143  GGCGCC               [GA]GCGC^[CT]
HgaI          829  GACGC                GACGC(5/10)
Hin1I         143  GGCGCC               G[GA]^CG[CT]C
HincII       2682  GTTGAC               GT[CT]^[GA]AC
HindII       2682  GTTGAC               GT[CT]^[GA]AC
KasI          143  GGCGCC               G^GCGCC
KspI          233  CCGCGG               CCGC^GG
MaeII         693  ACGT                 A^CGT
NaeI         1356  GCCGGC               GCC^GGC
NarI          143  GGCGCC               GG^CGCC
NgoMIV       1356  GCCGGC               G^CCGGC
NspV         4561  TTCGAA               TT^CGAA
PacI         2606  TTAATTAA             TTAAT^TAA
PflFI        2432  GACTTAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PinAI        2383  ACCGGT               A^CCGGT
SacII         233  CCGCGG               CCGC^GG
ScaI         3121  AGTACT               AGT^ACT
SfoI          143  GGCGCC               GGC^GCC
SfuI         4561  TTCGAA               TT^CGAA
SpeI         4969  ACTAGT               A^CTAGT
SspBI        3430  TGTACA               T^GTACA
Tth111I      2432  GACTTAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XbaI         3754  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

ApaI           52  GGGCCC               GGGCC^C
             3843  GGGCCC              
Asp700I       317  GAACCTGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             4499  GAAACATTTC          
BciVI        2639  GGATAC               GTATCC(6/5)
             1773  GTATCC              
BglII        3086  AGATCT               A^GATCT
             3619  AGATCT              
BsrBI          72  GAGCGG               CCGCTC(-3/-3)
              102  GAGCGG              
BsrDI        2687  CATTGC               GCAATG(2/0)
             4341  CATTGC              
BstEII        187  GGTCACC              G^GT[ACGT]ACC
             1102  GGTGACC             
BstPI         187  GGTCACC              G^GT[ACGT]ACC
             1102  GGTGACC             
DraI         2481  TTTAAA               TTT^AAA
             4424  TTTAAA              
DraIII        866  CACAGCGTG            CAC[ACGT][ACGT][ACGT]^GTG
             4572  CACCTTGTG           
DrdI          686  GACTGCTACGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             5068  GACATTGTAGTC        
EcoO65I       187  GGTCACC              G^GT[ACGT]ACC
             1102  GGTGACC             
EcoT22I      1596  ATGCAT               ATGCA^T
             5060  ATGCAT              
NdeI         4800  CATATG               CA^TATG
             5079  CATATG              
NsiI         1596  ATGCAT               ATGCA^T
             5060  ATGCAT              
SmaI           32  CCCGGG               CCC^GGG
               60  CCCGGG              
StuI         1221  AGGCCT               AGG^CCT
             3135  AGGCCT              
XmaCI          32  CCCGGG               C^CCGGG
               60  CCCGGG              
XmaI           32  CCCGGG               C^CCGGG
               60  CCCGGG              
XmnI          317  GAACCTGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             4499  GAAACATTTC          


#########################################
# 3 OR MORE CUT ENZYMES (  118 enzymes) #
#########################################

AccII       AciI        AcsI        AfaI        AluI        
AlwI        AlwNI       ApoI        AseI        AsnI        
AspHI       AvaI        AvaII       BalI        BanI        
BanII       BbsI        BbvI        BcnI        BfaI        
BmyI        BpmI        BpuAI       BsaI        BsaJI       
BseRI       BsgI        BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       BsmI        BsoBI       Bsp1286I    
BspHI       BspMI       BsrFI       BsrI        BssKI       
BstF5I      BstNI       BstUI       BstXI       BstYI       
Cac8I       CfoI        Cfr10I      Cfr13I      DdeI        
DpnII       DraII       DsaI        EaeI        EarI        
EcoNI       EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeIII      HapII       HhaI        
HinP1I      HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeIII      MboI        MboII       
MflI        MluNI       MnlI        MscI        MseI        
MslI        MspA1I      MspI        MvaI        MvnI        
MwoI        NciI        NcoI        NdeII       NlaIII      
NlaIV       NspI        PleI        PpuMI       PshBI       
PstI        PvuII       RcaI        RsaI        SacI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmlI        SspI        StyI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     XcmI        XhoII