##################################
# NO CUT ENZYMES (   53 enzymes) #
##################################

AatII       AccIII      AclI        AhdI        AscI        
AspEI       BseAI       BsiEI       BsiWI       Bsp1407I    
BspDI       BspEI       BsrBI       BsrGI       BssHII      
Bst1107I    BstZ17I     ClaI        CpoI        DraI        
DrdI        EagI        Eam1105I    EclXI       Eco52I      
FseI        KspI        MluI        MroI        NaeI        
NdeI        NgoMIV      NotI        NruI        PacI        
PaeR7I      PmeI        Psp1406I    PvuI        RsrII       
SacII       SalI        SbfI        SfiI        SmaI        
SnaBI       SpeI        Sse8387I    SspBI       SwaI        
XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

Acc65I       6894  GGTACC               G^GTACC
AccI         1344  GTCTAC               GT^[AC][GT]AC
AccII           4  CGCG                 CG^CG
Aor51HI      5296  AGCGCT               AGC^GCT
ApaI         6865  GGGCCC               GGGCC^C
AseI         1809  ATTAAT               AT^TAAT
AsnI         1809  ATTAAT               AT^TAAT
Asp718I      6894  GGTACC               G^GTACC
AspI         6193  GACTCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI         3730  CCCGAG               C^[CT]CG[GA]G
AviII        3565  TGCGCA               TGC^GCA
BamHI        6246  GGATCC               G^GATCC
BbeI         6073  GGCGCC               GGCGC^C
BclI          748  TGATCA               T^GATCA
BglII        1490  AGATCT               A^GATCT
BlpI         5020  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     5020  GCTCAGC              GC^T[ACGT]AGC
BsmBI        6031  CGTCTC               CGTCTC(1/5)
BsoBI        3730  CCCGAG               C^[CT]CG[GA]G
BstBI        4422  TTCGAA               TT^CGAA
BstUI           4  CGCG                 CG^CG
CelII        5020  GCTCAGC              GC^T[ACGT]AGC
Eco47III     5296  AGCGCT               AGC^GCT
EcoRV        3701  GATATC               GAT^ATC
FbaI          748  TGATCA               T^GATCA
FspI         3565  TGCGCA               TGC^GCA
HpaI         6333  GTTAAC               GTT^AAC
KasI         6073  GGCGCC               G^GCGCC
KpnI         6894  GGTACC               GGTAC^C
MfeI         2075  CAATTG               C^AATTG
MunI         2075  CAATTG               C^AATTG
MvnI            4  CGCG                 CG^CG
NarI         6073  GGCGCC               GG^CGCC
NspV         4422  TTCGAA               TT^CGAA
PflFI        6193  GACTCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PshAI        5157  GACCTGGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI        1809  ATTAAT               AT^TAAT
ScaI         3490  AGTACT               AGT^ACT
SfoI         6073  GGCGCC               GGC^GCC
SfuI         4422  TTCGAA               TT^CGAA
SgrAI        4056  CACCGGTG             C[GA]^CCGG[CT]G
SspI         4781  AATATT               AAT^ATT
Tth111I      6193  GACTCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XbaI         6278  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AcyI         3597  GGCGTC               G[GA]^CG[CT]C
             6073  GGCGCC              
AgeI         4057  ACCGGT               A^CCGGT
             4301  ACCGGT              
AvrII         225  CCTAGG               C^CTAGG
             6404  CCTAGG              
BbrPI        5666  CACGTG               CAC^GTG
             6646  CACGTG              
BcnI         2570  CCGGG                CC^[GC]GG
             3547  CCGGG               
BglI         2600  GCCCCAGCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             5611  GCCGCAATGGC         
BlnI          225  CCTAGG               C^CTAGG
             6404  CCTAGG              
BsaAI        5666  CACGTG               [CT]AC^GT[GA]
             6646  CACGTG              
BsaHI        3597  GGCGTC               G[GA]^CG[CT]C
             6073  GGCGCC              
BsaWI        4057  ACCGGT               [AT]^CCGG[AT]
             4301  ACCGGT              
BsmI         2399  GCATTC               GAATGC(1/-1)
             7040  GCATTC              
BspLU11I     5457  ACATGT               A^CATGT
             6386  ACATGT              
BssSI        5984  CACGAG               CACGAG(-5/-1)
             5590  CTCGTG              
BstAPI       3127  GCAAAGCTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             6979  GCAGCTTCTGC         
HgaI            2  GACGC                GACGC(5/10)
             3598  GCGTC               
Hin1I        3597  GGCGTC               G[GA]^CG[CT]C
             6073  GGCGCC              
NciI         2570  CCGGG                CC^[GC]GG
             3547  CCGGG               
NheI         1544  GCTAGC               G^CTAGC
             6967  GCTAGC              
PinAI        4057  ACCGGT               A^CCGGT
             4301  ACCGGT              
PmaCI        5666  CACGTG               CAC^GTG
             6646  CACGTG              
PmlI         5666  CACGTG               CAC^GTG
             6646  CACGTG              
SexAI         372  ACCAGGT              A^CC[AT]GGT
             5359  ACCTGGT             


#########################################
# 3 OR MORE CUT ENZYMES (  139 enzymes) #
#########################################

AciI        AcsI        AfaI        AflII       AflIII      
AluI        Alw44I      AlwI        AlwNI       AocI        
ApaLI       ApoI        Asp700I     AspHI       AvaII       
BalI        BanI        BanII       BbsI        BbvCI       
BbvI        BcgI        BciVI       BfaI        BfrI        
BmyI        BpmI        BpuAI       BsaBI       BsaI        
BsaJI       BseRI       BsgI        BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       Bsp1286I    BspHI       
BspMI       BsrDI       BsrFI       BsrI        BssKI       
BstEII      BstF5I      BstNI       BstPI       BstXI       
BstYI       Bsu36I      Cac8I       CfoI        Cfr10I      
Cfr13I      DdeI        DpnII       DraII       DraIII      
DsaI        EaeI        EarI        Eco81I      EcoNI       
EcoO109I    EcoO65I     EcoRI       EcoRII      EcoT14I     
EcoT22I     FauI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HhaI        HinP1I      HincII      
HindII      HindIII     HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeI        MaeII       MaeIII      
MamI        MboI        MboII       MflI        MluNI       
MnlI        MscI        MseI        MslI        MspA1I      
MspI        MvaI        MwoI        NcoI        NdeII       
NlaIII      NlaIV       NsiI        NspI        PflMI       
PleI        PpuMI       PstI        PvuII       RcaI        
RsaI        SacI        SapI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SmlI        SphI        
StuI        StyI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
Van91I      XcmI        XhoII       XmnI