##################################
# NO CUT ENZYMES ( 61 enzymes) #
##################################
Acc65I AflII AgeI AhdI Alw44I
AocI Aor51HI ApaLI AseI AsnI
Asp718I AspEI AvrII BamHI BciVI
BfrI BglII BlnI BsiWI BsmI
Bsp1407I BspLU11I BsrDI BsrGI Bst1107I
BstZ17I Bsu36I CpoI DraI DrdI
Eam1105I Eco47III Eco81I EcoRV EcoT22I
FseI KpnI MfeI MluI MunI
NheI NruI NsiI PacI PaeR7I
PinAI PmeI PshBI PvuI RsrII
SbfI ScaI SgrAI SnaBI SpeI
Sse8387I SspBI SspI SwaI XbaI
XhoI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AatII 3654 GACGTC GACGT^C
AccIII 3073 TCCGGA T^CCGGA
AclI 5454 AACGTT AA^CGTT
AcsI 755 GAATTC [GA]^AATT[CT]
ApoI 755 GAATTC [GA]^AATT[CT]
AscI 4825 GGCGCGCC GG^CGCGCC
Asp700I 3601 GAATCCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 3520 TGCGCA TGC^GCA
BbrPI 3039 CACGTG CAC^GTG
BclI 536 TGATCA T^GATCA
BlpI 3224 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 3224 GCTCAGC GC^T[ACGT]AGC
BsaBI 537 GATCATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 3073 TCCGGA T^CCGGA
BspDI 4 ATCGAT AT^CGAT
BspEI 3073 TCCGGA T^CCGGA
BspHI 539 TCATGA T^CATGA
BsrBI 20 GAGCGG CCGCTC(-3/-3)
BssHII 4826 GCGCGC G^CGCGC
BstBI 776 TTCGAA TT^CGAA
BstEII 2808 GGTGACC G^GT[ACGT]ACC
BstPI 2808 GGTGACC G^GT[ACGT]ACC
CelII 3224 GCTCAGC GC^T[ACGT]AGC
ClaI 4 ATCGAT AT^CGAT
DraIII 3980 CACCGAGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO65I 2808 GGTGACC G^GT[ACGT]ACC
EcoRI 755 GAATTC G^AATTC
FbaI 536 TGATCA T^GATCA
FspI 3520 TGCGCA TGC^GCA
HpaI 4808 GTTAAC GTT^AAC
MamI 537 GATCATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 3073 TCCGGA T^CCGGA
MslI 2999 CACGGCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NdeI 394 CATATG CA^TATG
NotI 4362 GCGGCCGC GC^GGCCGC
NspV 776 TTCGAA TT^CGAA
PflMI 858 CCAGCCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 3039 CACGTG CAC^GTG
PmlI 3039 CACGTG CAC^GTG
PshAI 2687 GACGCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 5454 AACGTT AA^CGTT
RcaI 539 TCATGA T^CATGA
SalI 368 GTCGAC G^TCGAC
SexAI 4309 ACCAGGT A^CC[AT]GGT
SfuI 776 TTCGAA TT^CGAA
SmlI 3699 CTCAAG C^T[CT][GA]AG
Van91I 858 CCAGCCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 3601 GAATCCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AfaI 4124 GTAC GT^AC
5076 GTAC
AflIII 4324 ACACGT A^C[GA][CT]GT
5418 ACGTGT
AspI 2531 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3387 GACGAGGTC
BbeI 4215 GGCGCC GGCGC^C
4571 GGCGCC
BcgI 4478 CGATGAGCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3686 GCAGCAGACTCG
BsaAI 3039 CACGTG [CT]AC^GT[GA]
4524 CACGTA
BsaI 4162 GAGACC GGTCTC(1/5)
2693 GGTCTC
BsrFI 101 GCCGGC [GA]^CCGG[CT]
4737 GCCGGC
BssSI 3170 CACGAG CACGAG(-5/-1)
4835 CACGAG
BstXI 4393 CCAAGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5216 CCAAAGCCCTGG
Cfr10I 101 GCCGGC [GA]^CCGG[CT]
4737 GCCGGC
KasI 4215 GGCGCC G^GCGCC
4571 GGCGCC
KspI 122 CCGCGG CCGC^GG
4042 CCGCGG
MseI 4809 TTAA T^TAA
5446 TTAA
NaeI 101 GCCGGC GCC^GGC
4737 GCCGGC
NarI 4215 GGCGCC GG^CGCC
4571 GGCGCC
NcoI 3814 CCATGG C^CATGG
3922 CCATGG
NgoMIV 101 GCCGGC G^CCGGC
4737 GCCGGC
PflFI 2531 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3387 GACGAGGTC
RsaI 4124 GTAC GT^AC
5076 GTAC
SacII 122 CCGCGG CCGC^GG
4042 CCGCGG
SapI 4172 GAAGAGC GCTCTTC(1/4)
4517 GAAGAGC
SfoI 4215 GGCGCC GGC^GCC
4571 GGCGCC
SphI 3016 GCATGC GCATG^C
4153 GCATGC
StuI 3841 AGGCCT AGG^CCT
4768 AGGCCT
Tru9I 4809 TTAA T^TAA
5446 TTAA
Tth111I 2531 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3387 GACGAGGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 122 enzymes) #
#########################################
AccI AccII AciI AcyI AluI
AlwI AlwNI ApaI AspHI AvaI
AvaII BalI BanI BanII BbsI
BbvCI BbvI BcnI BfaI BglI
BmyI BpmI BpuAI BsaHI BsaJI
BsaWI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I BspMI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EagI EarI
EclXI Eco52I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PpuMI
PstI PvuII SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SfiI SmaI
StyI TaqI TfiI TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XhoII
XmaCI XmaI