##################################
# NO CUT ENZYMES ( 52 enzymes) #
##################################
AccIII AclI AflII AscI AseI
AsnI AvrII BclI BfrI BlnI
BsaI BseAI BspEI BspHI BspLU11I
Bst1107I BstXI BstZ17I CpoI DrdI
EcoRV EcoT22I FbaI HincII HindII
HpaI MfeI MluI MroI MunI
NdeI NheI NruI NsiI PacI
PmeI PshAI PshBI Psp1406I PvuI
RcaI RsrII SalI SbfI ScaI
SfiI SnaBI SpeI Sse8387I SspI
SwaI XbaI
###################################
# ONE CUT ENZYMES ( 23 enzymes) #
###################################
AflIII 1667 ACGTGT A^C[GA][CT]GT
AgeI 1824 ACCGGT A^CCGGT
BbrPI 2344 CACGTG CAC^GTG
BciVI 3531 GGATAC GTATCC(6/5)
BsaBI 4740 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiWI 2778 CGTACG C^GTACG
BspDI 5334 ATCGAT AT^CGAT
BssHII 5592 GCGCGC G^CGCGC
BstBI 3301 TTCGAA TT^CGAA
ClaI 5334 ATCGAT AT^CGAT
DraI 6336 TTTAAA TTT^AAA
FseI 1588 GGCCGGCC GGCCGG^CC
HindIII 5446 AAGCTT A^AGCTT
MamI 4740 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NotI 4879 GCGGCCGC GC^GGCCGC
NspV 3301 TTCGAA TT^CGAA
PaeR7I 4912 CTCGAG C^TCGAG
PinAI 1824 ACCGGT A^CCGGT
PmaCI 2344 CACGTG CAC^GTG
PmlI 2344 CACGTG CAC^GTG
SfuI 3301 TTCGAA TT^CGAA
SgrAI 1823 CACCGGTG C[GA]^CCGG[CT]G
XhoI 4912 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AatII 3857 GACGTC GACGT^C
5401 GACGTC
Acc65I 3634 GGTACC G^GTACC
3991 GGTACC
AccI 1831 GTCTAC GT^[AC][GT]AC
5128 GTCTAC
Aor51HI 1231 AGCGCT AGC^GCT
3821 AGCGCT
Asp718I 3634 GGTACC G^GTACC
3991 GGTACC
AviII 1712 TGCGCA TGC^GCA
3131 TGCGCA
BamHI 305 GGATCC G^GATCC
1985 GGATCC
BglII 884 AGATCT A^GATCT
5354 AGATCT
BsmBI 491 GAGACG CGTCTC(1/5)
5698 GAGACG
BsmI 283 GAATGC GAATGC(1/-1)
3394 GCATTC
BsrBI 978 CCGCTC CCGCTC(-3/-3)
634 GAGCGG
DraIII 2341 CACCACGTG CAC[ACGT][ACGT][ACGT]^GTG
3169 CACTACGTG
Eco47III 1231 AGCGCT AGC^GCT
3821 AGCGCT
EcoRI 241 GAATTC G^AATTC
3139 GAATTC
FspI 1712 TGCGCA TGC^GCA
3131 TGCGCA
KpnI 3634 GGTACC GGTAC^C
3991 GGTACC
SexAI 233 ACCTGGT A^CC[AT]GGT
4804 ACCTGGT
SphI 1184 GCATGC GCATG^C
2099 GCATGC
TfiI 1605 GATTC G^A[AT]TC
4050 GAATC
#########################################
# 3 OR MORE CUT ENZYMES ( 164 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AhdI AluI Alw44I AlwI AlwNI
AocI ApaI ApaLI ApoI Asp700I
AspEI AspHI AspI AvaI AvaII
BalI BanI BanII BbeI BbsI
BbvCI BbvI BcgI BcnI BfaI
BglI BlpI BmyI BpmI Bpu1102I
BpuAI BsaAI BsaHI BsaJI BsaWI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
Bsp1407I BspMI BsrDI BsrFI BsrGI
BsrI BssKI BssSI BstAPI BstEII
BstF5I BstNI BstPI BstUI BstYI
Bsu36I Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EagI Eam1105I EarI EclXI
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI KasI Ksp632I KspI MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NarI NciI NcoI NdeII
NgoMIV NlaIII NlaIV NspI PflFI
PflMI PleI PpuMI PstI PvuII
RsaI SacI SacII SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SfoI
SmaI SmlI SspBI StuI StyI
TaqI Tru9I TseI Tsp45I Tsp509I
TspRI Tth111I TthHB8I Van91I XcmI
XhoII XmaCI XmaI XmnI