##################################
# NO CUT ENZYMES ( 60 enzymes) #
##################################
AatII AccIII AclI AscI AseI
AsnI Asp700I AvrII BclI BlnI
BseAI BsiWI BsmI Bsp1407I BspDI
BspEI BspLU11I BsrGI BssSI BstBI
BstEII BstPI ClaI DraIII EcoO65I
EcoRI FbaI FseI HincII HindII
HpaI MfeI MluI MroI MunI
NaeI NdeI NgoMIV NheI NotI
NruI NspV PacI PmeI PshAI
PshBI Psp1406I SalI SbfI SexAI
SfiI SfuI SgrAI SnaBI SpeI
Sse8387I SspBI SspI SwaI XmnI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
Acc65I 1905 GGTACC G^GTACC
AflII 381 CTTAAG C^TTAAG
AflIII 1224 ACGTGT A^C[GA][CT]GT
AgeI 1932 ACCGGT A^CCGGT
AocI 1980 CCTCAGG CC^T[ACGT]AGG
ApaI 3424 GGGCCC GGGCC^C
Asp718I 1905 GGTACC G^GTACC
AspI 878 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1330 TGCGCA TGC^GCA
BamHI 742 GGATCC G^GATCC
BbrPI 1741 CACGTG CAC^GTG
BbvCI 1393 GCTGAGG CCTCAGC(-5/-2)
BciVI 1923 GGATAC GTATCC(6/5)
BfrI 381 CTTAAG C^TTAAG
BlpI 902 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 902 GCTGAGC GC^T[ACGT]AGC
BsaBI 2368 GATGGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BspHI 2433 TCATGA T^CATGA
BsrFI 1932 ACCGGT [GA]^CCGG[CT]
Bst1107I 4445 GTATAC GTA^TAC
BstAPI 729 GCAGAGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 4445 GTATAC GTA^TAC
Bsu36I 1980 CCTCAGG CC^T[ACGT]AGG
CelII 902 GCTGAGC GC^T[ACGT]AGC
Cfr10I 1932 ACCGGT [GA]^CCGG[CT]
DrdI 2560 GACACGGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1133 CGGCCG C^GGCCG
EclXI 1133 CGGCCG C^GGCCG
Eco52I 1133 CGGCCG C^GGCCG
Eco81I 1980 CCTCAGG CC^T[ACGT]AGG
EcoNI 632 CCTCGGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 2603 GATATC GAT^ATC
FspI 1330 TGCGCA TGC^GCA
HindIII 4194 AAGCTT A^AGCTT
KpnI 1905 GGTACC GGTAC^C
MamI 2368 GATGGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
PflFI 878 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 1932 ACCGGT A^CCGGT
PmaCI 1741 CACGTG CAC^GTG
PmlI 1741 CACGTG CAC^GTG
PvuI 491 CGATCG CGAT^CG
RcaI 2433 TCATGA T^CATGA
SacI 127 GAGCTC GAGCT^C
SapI 683 GCTCTTC GCTCTTC(1/4)
Tth111I 878 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 4490 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AlwNI 717 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
730 CAGAGCCTG
BcgI 2012 CGAAACCACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1685 GCAATGAACTCG
BsaAI 1741 CACGTG [CT]AC^GT[GA]
4017 CACGTA
BsgI 1541 CTGCAC GTGCAG(16/14)
3039 CTGCAC
BsiEI 491 CGATCG CG[GA][CT]^CG
1133 CGGCCG
CpoI 1491 CGGACCG CG^G[AT]CCG
1503 CGGACCG
DraI 2258 TTTAAA TTT^AAA
4546 TTTAAA
EcoT22I 556 ATGCAT ATGCA^T
1212 ATGCAT
KspI 860 CCGCGG CCGC^GG
1557 CCGCGG
NsiI 556 ATGCAT ATGCA^T
1212 ATGCAT
RsrII 1491 CGGACCG CG^G[AT]CCG
1503 CGGACCG
SacII 860 CCGCGG CCGC^GG
1557 CCGCGG
ScaI 2159 AGTACT AGT^ACT
3026 AGTACT
SphI 554 GCATGC GCATG^C
2062 GCATGC
StuI 977 AGGCCT AGG^CCT
3035 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 137 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AhdI AluI Alw44I AlwI
Aor51HI ApaLI ApoI AspEI AspHI
AvaI AvaII BalI BanI BanII
BbeI BbsI BbvI BcnI BfaI
BglI BglII BmyI BpmI BpuAI
BsaHI BsaI BsaJI BsaWI BseRI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrDI BsrI BssHII BssKI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI Eam1105I EarI Eco47III
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI KasI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NarI NciI NcoI NdeII NlaIII
NlaIV NspI PaeR7I PflMI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SfoI
SmaI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoI XhoII
XmaCI XmaI