##################################
# NO CUT ENZYMES ( 75 enzymes) #
##################################
AatII AccIII AclI AflII AgeI
AhdI ApaI AscI AseI AsnI
AspEI BcgI BciVI BclI BfrI
BglI BglII BlpI Bpu1102I BsaI
BsaWI BseAI BsiWI BsmBI BspDI
BspEI BspLU11I BspMI BsrBI BssHII
BssSI Bst1107I BstAPI BstBI BstXI
BstZ17I CelII ClaI CpoI Eam1105I
FbaI FseI HgaI HincII HindII
HpaI MluI MroI NcoI NheI
NotI NruI NspV PacI PaeR7I
PinAI PmeI PshAI PshBI Psp1406I
PvuI RsrII SalI SbfI ScaI
SexAI SfiI SfuI SgrAI SnaBI
SpeI Sse8387I SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
Acc65I 184 GGTACC G^GTACC
AflIII 2092 ACACGT A^C[GA][CT]GT
Aor51HI 72 AGCGCT AGC^GCT
Asp718I 184 GGTACC G^GTACC
AviII 94 TGCGCA TGC^GCA
AvrII 3957 CCTAGG C^CTAGG
BalI 3253 TGGCCA TGG^CCA
BamHI 991 GGATCC G^GATCC
BbrPI 2093 CACGTG CAC^GTG
BlnI 3957 CCTAGG C^CTAGG
BsaAI 2093 CACGTG [CT]AC^GT[GA]
BsiEI 6 CGGCCG CG[GA][CT]^CG
BsmI 2349 GCATTC GAATGC(1/-1)
Bsp1407I 2524 TGTACA T^GTACA
BsrDI 1722 CATTGC GCAATG(2/0)
BsrFI 140 GCCGGC [GA]^CCGG[CT]
BsrGI 2524 TGTACA T^GTACA
BstEII 193 GGTCACC G^GT[ACGT]ACC
BstPI 193 GGTCACC G^GT[ACGT]ACC
Cfr10I 140 GCCGGC [GA]^CCGG[CT]
DraIII 1251 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2694 GACAACGTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 6 CGGCCG C^GGCCG
EclXI 6 CGGCCG C^GGCCG
Eco47III 72 AGCGCT AGC^GCT
Eco52I 6 CGGCCG C^GGCCG
EcoNI 1643 CCTACTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 193 GGTCACC G^GT[ACGT]ACC
EcoRI 2543 GAATTC G^AATTC
EcoT22I 623 ATGCAT ATGCA^T
FspI 94 TGCGCA TGC^GCA
KpnI 184 GGTACC GGTAC^C
MluNI 3253 TGGCCA TGG^CCA
MscI 3253 TGGCCA TGG^CCA
NaeI 140 GCCGGC GCC^GGC
NgoMIV 140 GCCGGC G^CCGGC
NsiI 623 ATGCAT ATGCA^T
PmaCI 2093 CACGTG CAC^GTG
PmlI 2093 CACGTG CAC^GTG
PvuII 3249 CAGCTG CAG^CTG
SmaI 135 CCCGGG CCC^GGG
SphI 2516 GCATGC GCATG^C
SspBI 2524 TGTACA T^GTACA
XcmI 2241 CCAAAGAAAAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 135 CCCGGG C^CCGGG
XmaI 135 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AccI 1077 GTAGAC GT^[AC][GT]AC
2721 GTCTAC
AccII 50 CGCG CG^CG
81 CGCG
AcyI 25 GGCGCC G[GA]^CG[CT]C
54 GGCGCC
Alw44I 130 GTGCAC G^TGCAC
605 GTGCAC
AlwNI 2739 CAGACCCTG CAG[ACGT][ACGT][ACGT]^CTG
2906 CAGGCTCTG
ApaLI 130 GTGCAC G^TGCAC
605 GTGCAC
Asp700I 2081 GAAGTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3506 GAAAATATTC
AspI 2694 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
3065 GACTCTGTC
BbeI 25 GGCGCC GGCGC^C
54 GGCGCC
BbvCI 267 CCTCAGC CCTCAGC(-5/-2)
458 CCTCAGC
BsaBI 581 GATGGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2643 GATTTGCATC
BsaHI 25 GGCGCC G[GA]^CG[CT]C
54 GGCGCC
BstUI 50 CGCG CG^CG
81 CGCG
DraI 2189 TTTAAA TTT^AAA
3986 TTTAAA
DraII 640 GGGACCT [GA]G^G[ACGT]CC[CT]
3258 AGGACCC
EcoO109I 640 GGGACCT [GA]G^G[ACGT]CC[CT]
3258 AGGACCC
EcoRV 655 GATATC GAT^ATC
1544 GATATC
Hin1I 25 GGCGCC G[GA]^CG[CT]C
54 GGCGCC
HindIII 1143 AAGCTT A^AGCTT
1188 AAGCTT
KasI 25 GGCGCC G^GCGCC
54 GGCGCC
KspI 49 CCGCGG CCGC^GG
80 CCGCGG
MaeII 2094 ACGT A^CGT
2698 ACGT
MamI 581 GATGGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2643 GATTTGCATC
MfeI 1897 CAATTG C^AATTG
2258 CAATTG
MslI 1378 CATTTGTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2809 CACATCCGTG
MunI 1897 CAATTG C^AATTG
2258 CAATTG
MvnI 50 CGCG CG^CG
81 CGCG
NarI 25 GGCGCC GG^CGCC
54 GGCGCC
PflFI 2694 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
3065 GACTCTGTC
PflMI 1250 CCACCAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1629 CCATTAAATGG
PpuMI 640 GGGACCT [GA]G^G[AT]CC[CT]
3258 AGGACCC
SacII 49 CCGCGG CCGC^GG
80 CCGCGG
SfoI 25 GGCGCC GGC^GCC
54 GGCGCC
Tth111I 2694 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
3065 GACTCTGTC
Van91I 1250 CCACCAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1629 CCATTAAATGG
XmnI 2081 GAAGTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3506 GAAAATATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 101 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AocI ApoI AspHI AvaI AvaII
BanI BanII BbsI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspHI
BsrI BssKI BstF5I BstNI BstYI
Bsu36I Cac8I CfoI Cfr13I DdeI
DpnII DsaI EaeI EarI Eco81I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MflI
MnlI MseI MspA1I MspI MvaI
MwoI NciI NdeI NdeII NlaIII
NlaIV NspI PleI PstI RcaI
RsaI SacI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII