##################################
# NO CUT ENZYMES (   78 enzymes) #
##################################

AatII       Acc65I      AccII       AclI        AcyI        
AflIII      AgeI        Alw44I      Aor51HI     ApaI        
ApaLI       AscI        Asp718I     AviII       AvrII       
BbeI        BbrPI       BglII       BlnI        BlpI        
Bpu1102I    BsaHI       BsiWI       BsmBI       BspLU11I    
BsrBI       BssHII      BssSI       Bst1107I    BstAPI      
BstEII      BstPI       BstUI       BstZ17I     CelII       
CpoI        DrdI        EagI        EclXI       Eco47III    
Eco52I      EcoNI       EcoO65I     FseI        FspI        
Hin1I       KasI        KpnI        KspI        MluI        
MvnI        NaeI        NarI        NgoMIV      NheI        
NotI        NruI        PacI        PinAI       PmaCI       
PmeI        PmlI        PshAI       Psp1406I    PvuI        
RsrII       SacII       SalI        SapI        SbfI        
ScaI        SexAI       SfiI        SfoI        SgrAI       
SnaBI       SpeI        Sse8387I    


###################################
# ONE CUT ENZYMES (   50 enzymes) #
###################################

AccIII       2037  TCCGGA               T^CCGGA
AflII         465  CTTAAG               C^TTAAG
AhdI         3329  GACTAAAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI        3329  GACTAAAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI         175  GGATCC               G^GATCC
BanI         1714  GGTGCC               G^G[CT][GA]CC
BanII        4059  GAGCTC               G[GA]GC[CT]^C
BbsI          640  GAAGAC               GAAGAC(2/6)
BcgI         1271  GCAAAAACATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI          465  CTTAAG               C^TTAAG
BglI         2397  GCCTGATGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI         640  GAAGAC               GAAGAC(2/6)
BsaAI        1255  TACGTG               [CT]AC^GT[GA]
BsaI           24  GAGACC               GGTCTC(1/5)
BseAI        2037  TCCGGA               T^CCGGA
BseRI        1861  GAGGAG               GAGGAG(10/8)
BsgI         2784  GTGCAG               GTGCAG(16/14)
BsiEI        1021  CGACCG               CG[GA][CT]^CG
BspDI         931  ATCGAT               AT^CGAT
BspEI        2037  TCCGGA               T^CCGGA
BspMI        1592  GCAGGT               ACCTGC(4/8)
BsrFI         350  ACCGGC               [GA]^CCGG[CT]
BstBI         471  TTCGAA               TT^CGAA
BstXI        1448  CCACAGAAATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI         1445  GCGC                 GCG^C
Cfr10I        350  ACCGGC               [GA]^CCGG[CT]
ClaI          931  ATCGAT               AT^CGAT
DraIII       3212  CACAGAGTG            CAC[ACGT][ACGT][ACGT]^GTG
DsaI          760  CCATGG               C^C[GA][CT]GG
Eam1105I     3329  GACTAAAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI        2890  GAATTC               G^AATTC
EcoT22I      3939  ATGCAT               ATGCA^T
HaeII        1444  AGCGCC               [GA]GCGC^[CT]
HhaI         1445  GCGC                 GCG^C
HinP1I       1445  GCGC                 G^CGC
MfeI         2637  CAATTG               C^AATTG
MroI         2037  TCCGGA               T^CCGGA
MunI         2637  CAATTG               C^AATTG
NcoI          760  CCATGG               C^CATGG
NsiI         3939  ATGCAT               ATGCA^T
NspV          471  TTCGAA               TT^CGAA
PaeR7I        550  CTCGAG               C^TCGAG
PvuII        1900  CAGCTG               CAG^CTG
SacI         4059  GAGCTC               GAGCT^C
SfuI          471  TTCGAA               TT^CGAA
SphI         3937  GCATGC               GCATG^C
SwaI         3608  ATTTAAAT             ATTT^AAAT
XbaI          478  TCTAGA               T^CTAGA
XcmI          751  CCAGGCGTACCATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI          550  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   34 enzymes) #
###################################

AocI          131  CCTCAGG              CC^T[ACGT]AGG
              170  CCTCAGG             
Asp700I      2502  GAAGAGTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             3220  GAAATATTTC          
AspHI        2861  GAGCAC               G[AT]GC[AT]^C
             4059  GAGCTC              
AspI         1563  GACTGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1938  GACCCCGTC           
BalI         1457  TGGCCA               TGG^CCA
             1995  TGGCCA              
BbvCI        2215  CCTCAGC              CCTCAGC(-5/-2)
              424  GCTGAGG             
BciVI        1223  GGATAC               GTATCC(6/5)
             1315  GGATAC              
BmyI         2861  GAGCAC               G[AGT]GC[ACT]^C
             4059  GAGCTC              
BpmI          749  CTCCAG               CTGGAG(16/14)
             1890  CTGGAG              
BsaBI         775  GATTCCAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             2263  GATGGATATC          
BsiHKAI      2861  GAGCAC               G[AT]GC[AT]^C
             4059  GAGCTC              
Bsp1286I     2861  GAGCAC               G[AGT]GC[ACT]^C
             4059  GAGCTC              
Bsp1407I     2466  TGTACA               T^GTACA
             3152  TGTACA              
BsrGI        2466  TGTACA               T^GTACA
             3152  TGTACA              
Bsu36I        131  CCTCAGG              CC^T[ACGT]AGG
              170  CCTCAGG             
EaeI         1457  TGGCCA               [CT]^GGCC[GA]
             1995  TGGCCA              
Eco81I        131  CCTCAGG              CC^T[ACGT]AGG
              170  CCTCAGG             
EcoRV         895  GATATC               GAT^ATC
             2267  GATATC              
HgaI          500  GACGC                GACGC(5/10)
              342  GCGTC               
HindIII      1142  AAGCTT               A^AGCTT
             1179  AAGCTT              
HpaI         2713  GTTAAC               GTT^AAC
             4083  GTTAAC              
MamI          775  GATTCCAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             2263  GATGGATATC          
MluNI        1457  TGGCCA               TGG^CCA
             1995  TGGCCA              
MscI         1457  TGGCCA               TGG^CCA
             1995  TGGCCA              
MspA1I        307  CAGCGG               C[AC]G^C[GT]G
             1900  CAGCTG              
NdeI         2470  CATATG               CA^TATG
             3156  CATATG              
PflFI        1563  GACTGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1938  GACCCCGTC           
PstI         1883  CTGCAG               CTGCA^G
             2422  CTGCAG              
SmaI          188  CCCGGG               CCC^GGG
             2207  CCCGGG              
SspBI        2466  TGTACA               T^GTACA
             3152  TGTACA              
Tth111I      1563  GACTGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1938  GACCCCGTC           
XmaCI         188  CCCGGG               C^CCGGG
             2207  CCCGGG              
XmaI          188  CCCGGG               C^CCGGG
             2207  CCCGGG              
XmnI         2502  GAAGAGTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             3220  GAAATATTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   96 enzymes) #
#########################################

AccI        AciI        AcsI        AfaI        AluI        
AlwI        AlwNI       ApoI        AseI        AsnI        
AvaI        AvaII       BbvI        BclI        BcnI        
BfaI        BsaJI       BsaWI       BsiYI       BslI        
BsmAI       BsmFI       BsmI        BsoBI       BspHI       
BsrDI       BsrI        BssKI       BstF5I      BstNI       
BstYI       Cac8I       Cfr13I      DdeI        DpnII       
DraI        DraII       EarI        EcoO109I    EcoRII      
EcoT14I     FauI        FbaI        Fnu4HI      FokI        
HaeIII      HapII       HincII      HindII      HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeI        
MaeII       MaeIII      MboI        MboII       MflI        
MnlI        MseI        MslI        MspI        MvaI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
NspI        PflMI       PleI        PpuMI       PshBI       
RcaI        RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SmlI        SspI        StuI        
StyI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     Van91I      
XhoII