##################################
# NO CUT ENZYMES ( 76 enzymes) #
##################################
AatII Acc65I AccIII AflII AgeI
AhdI AscI Asp700I Asp718I AspEI
AviII AvrII BbeI BbrPI BciVI
BfrI BlnI BsaAI BsaBI BsaWI
BseAI BsiWI BsmBI BsmI BspEI
BspLU11I BssHII Bst1107I BstBI BstEII
BstPI BstZ17I CpoI DraIII Eam1105I
EcoO65I EcoT22I FseI FspI KasI
KpnI KspI MamI MfeI MluI
MroI MunI NarI NdeI NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PvuI
RsrII SacI SacII SbfI SexAI
SfiI SfoI SfuI SgrAI SnaBI
SpeI Sse8387I SwaI XbaI XhoI
XmnI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AclI 134 AACGTT AA^CGTT
AflIII 2958 ACGTGT A^C[GA][CT]GT
Aor51HI 1685 AGCGCT AGC^GCT
ApaI 1555 GGGCCC GGGCC^C
AseI 3366 ATTAAT AT^TAAT
AsnI 3366 ATTAAT AT^TAAT
BalI 1562 TGGCCA TGG^CCA
BanI 847 GGCACC G^G[CT][GA]CC
BglI 713 GCCACTCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsiEI 1519 CGGCCG CG[GA][CT]^CG
BspHI 3873 TCATGA T^CATGA
BssSI 1513 CACGAG CACGAG(-5/-1)
BstAPI 2571 GCATGAAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DrdI 1449 GACACTGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1519 CGGCCG C^GGCCG
EclXI 1519 CGGCCG C^GGCCG
Eco47III 1685 AGCGCT AGC^GCT
Eco52I 1519 CGGCCG C^GGCCG
EcoRI 574 GAATTC G^AATTC
EcoRV 3678 GATATC GAT^ATC
HindIII 4043 AAGCTT A^AGCTT
HpaI 4504 GTTAAC GTT^AAC
MluNI 1562 TGGCCA TGG^CCA
MscI 1562 TGGCCA TGG^CCA
NheI 4146 GCTAGC G^CTAGC
PshAI 4011 GACTAAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 3366 ATTAAT AT^TAAT
Psp1406I 134 AACGTT AA^CGTT
RcaI 3873 TCATGA T^CATGA
SalI 3302 GTCGAC G^TCGAC
SmaI 1719 CCCGGG CCC^GGG
SmlI 2524 CTCAAG C^T[CT][GA]AG
SphI 2448 GCATGC GCATG^C
SspI 4287 AATATT AAT^ATT
XcmI 2996 CCAAAGAACTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 1719 CCCGGG C^CCGGG
XmaI 1719 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
Alw44I 376 GTGCAC G^TGCAC
1921 GTGCAC
AocI 266 CCTCAGG CC^T[ACGT]AGG
4792 CCTCAGG
ApaLI 376 GTGCAC G^TGCAC
1921 GTGCAC
AspI 1999 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
4861 GACCCTGTC
AvaI 923 CCCGAG C^[CT]CG[GA]G
1719 CCCGGG
BamHI 2894 GGATCC G^GATCC
3179 GGATCC
BbvCI 1008 CCTCAGC CCTCAGC(-5/-2)
1650 CCTCAGC
BcgI 4050 CGATAATCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
735 GCATCAGCTTCG
BclI 363 TGATCA T^GATCA
3473 TGATCA
BglII 2636 AGATCT A^GATCT
3342 AGATCT
BlpI 124 GCTAAGC GC^T[ACGT]AGC
757 GCTCAGC
Bpu1102I 124 GCTAAGC GC^T[ACGT]AGC
757 GCTCAGC
BsaI 4672 GAGACC GGTCTC(1/5)
690 GGTCTC
BsoBI 923 CCCGAG C^[CT]CG[GA]G
1719 CCCGGG
Bsp1407I 4448 TGTACA T^GTACA
4553 TGTACA
BspDI 2665 ATCGAT AT^CGAT
2984 ATCGAT
BsrBI 1507 GAGCGG CCGCTC(-3/-3)
1516 GAGCGG
BsrDI 625 GCAATG GCAATG(2/0)
1868 GCAATG
BsrGI 4448 TGTACA T^GTACA
4553 TGTACA
BstXI 653 CCATCGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4622 CCAGCACTTTGG
Bsu36I 266 CCTCAGG CC^T[ACGT]AGG
4792 CCTCAGG
CelII 124 GCTAAGC GC^T[ACGT]AGC
757 GCTCAGC
ClaI 2665 ATCGAT AT^CGAT
2984 ATCGAT
DsaI 2357 CCATGG C^C[GA][CT]GG
4113 CCATGG
Eco81I 266 CCTCAGG CC^T[ACGT]AGG
4792 CCTCAGG
FbaI 363 TGATCA T^GATCA
3473 TGATCA
NcoI 2357 CCATGG C^CATGG
4113 CCATGG
PflFI 1999 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
4861 GACCCTGTC
PflMI 2301 CCAGAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3525 CCAGTTTGTGG
SapI 1956 GAAGAGC GCTCTTC(1/4)
6 GCTCTTC
ScaI 1946 AGTACT AGT^ACT
3860 AGTACT
SspBI 4448 TGTACA T^GTACA
4553 TGTACA
StuI 771 AGGCCT AGG^CCT
2612 AGGCCT
Tth111I 1999 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
4861 GACCCTGTC
Van91I 2301 CCAGAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3525 CCAGTTTGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 110 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AluI AlwI AlwNI ApoI
AspHI AvaII BanII BbsI BbvI
BcnI BfaI BmyI BpmI BpuAI
BsaHI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BsrFI BsrI BssKI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraI
DraII EaeI EarI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NciI
NdeII NgoMIV NlaIII NlaIV NspI
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII