##################################
# NO CUT ENZYMES ( 54 enzymes) #
##################################
AatII Acc65I AflII AhdI AscI
Asp718I AspEI AviII BclI BfrI
BsiWI BsmBI BsmI BspDI BsrBI
BssHII Bst1107I BstZ17I ClaI CpoI
DrdI EagI Eam1105I EclXI Eco52I
EcoRV FbaI FseI FspI HincII
HindII HpaI KpnI KspI MfeI
MluI MunI NaeI NgoMIV NotI
NruI PacI PmeI PvuI RsrII
SacII SalI SbfI SfiI SgrAI
SpeI SphI Sse8387I SwaI
###################################
# ONE CUT ENZYMES ( 63 enzymes) #
###################################
AccII 4295 CGCG CG^CG
AccIII 1724 TCCGGA T^CCGGA
AclI 3734 AACGTT AA^CGTT
AgeI 50 ACCGGT A^CCGGT
Aor51HI 5709 AGCGCT AGC^GCT
AspI 5046 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 4647 TGGCCA TGG^CCA
BamHI 331 GGATCC G^GATCC
BbeI 4868 GGCGCC GGCGC^C
BbrPI 3123 CACGTG CAC^GTG
BbvCI 5016 GCTGAGG CCTCAGC(-5/-2)
BcgI 5705 GCACAGCGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI 5280 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 5280 GCTGAGC GC^T[ACGT]AGC
BsaBI 1567 GATCTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 1724 TCCGGA T^CCGGA
Bsp1407I 2 TGTACA T^GTACA
BspEI 1724 TCCGGA T^CCGGA
BspHI 3550 TCATGA T^CATGA
BspMI 3514 ACCTGC ACCTGC(4/8)
BsrFI 50 ACCGGT [GA]^CCGG[CT]
BsrGI 2 TGTACA T^GTACA
BssSI 1609 CACGAG CACGAG(-5/-1)
BstBI 297 TTCGAA TT^CGAA
BstUI 4295 CGCG CG^CG
CelII 5280 GCTGAGC GC^T[ACGT]AGC
Cfr10I 50 ACCGGT [GA]^CCGG[CT]
DraIII 169 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 5709 AGCGCT AGC^GCT
EcoRI 3303 GAATTC G^AATTC
HgaI 594 GCGTC GACGC(5/10)
HindIII 5757 AAGCTT A^AGCTT
KasI 4868 GGCGCC G^GCGCC
MamI 1567 GATCTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 4647 TGGCCA TGG^CCA
MroI 1724 TCCGGA T^CCGGA
MscI 4647 TGGCCA TGG^CCA
MslI 5469 CACCATGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 4295 CGCG CG^CG
NarI 4868 GGCGCC GG^CGCC
NheI 5672 GCTAGC G^CTAGC
NspV 297 TTCGAA TT^CGAA
PflFI 5046 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 755 CCAAAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 50 ACCGGT A^CCGGT
PmaCI 3123 CACGTG CAC^GTG
PmlI 3123 CACGTG CAC^GTG
PshAI 3577 GACAAGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 3734 AACGTT AA^CGTT
RcaI 3550 TCATGA T^CATGA
SacI 5540 GAGCTC GAGCT^C
SexAI 483 ACCAGGT A^CC[AT]GGT
SfoI 4868 GGCGCC GGC^GCC
SfuI 297 TTCGAA TT^CGAA
SmaI 68 CCCGGG CCC^GGG
SnaBI 4140 TACGTA TAC^GTA
SspBI 2 TGTACA T^GTACA
SspI 2026 AATATT AAT^ATT
Tth111I 5046 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 755 CCAAAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 3362 TCTAGA T^CTAGA
XmaCI 68 CCCGGG C^CCGGG
XmaI 68 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccI 4077 GTAGAC GT^[AC][GT]AC
4143 GTAGAC
AcyI 593 GGCGTC G[GA]^CG[CT]C
4868 GGCGCC
Alw44I 3120 GTGCAC G^TGCAC
5312 GTGCAC
AocI 538 CCTCAGG CC^T[ACGT]AGG
5650 CCTCAGG
ApaI 71 GGGCCC GGGCC^C
4412 GGGCCC
ApaLI 3120 GTGCAC G^TGCAC
5312 GTGCAC
AseI 1246 ATTAAT AT^TAAT
3206 ATTAAT
AsnI 1246 ATTAAT AT^TAAT
3206 ATTAAT
Asp700I 1399 GAAATTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1744 GAAATCCTTC
AvrII 1137 CCTAGG C^CTAGG
4517 CCTAGG
BlnI 1137 CCTAGG C^CTAGG
4517 CCTAGG
BsaAI 3123 CACGTG [CT]AC^GT[GA]
4140 TACGTA
BsaHI 593 GGCGTC G[GA]^CG[CT]C
4868 GGCGCC
BsaI 5593 GAGACC GGTCTC(1/5)
4420 GGTCTC
BsaWI 50 ACCGGT [AT]^CCGG[AT]
1724 TCCGGA
BsiEI 48 CGACCG CG[GA][CT]^CG
52 CGGTCG
BspLU11I 3832 ACATGT A^CATGT
4056 ACATGT
BsrDI 5532 CATTGC GCAATG(2/0)
1004 GCAATG
Bsu36I 538 CCTCAGG CC^T[ACGT]AGG
5650 CCTCAGG
Eco81I 538 CCTCAGG CC^T[ACGT]AGG
5650 CCTCAGG
EcoT22I 1833 ATGCAT ATGCA^T
3380 ATGCAT
Hin1I 593 GGCGTC G[GA]^CG[CT]C
4868 GGCGCC
NsiI 1833 ATGCAT ATGCA^T
3380 ATGCAT
PaeR7I 1193 CTCGAG C^TCGAG
5713 CTCGAG
PshBI 1246 ATTAAT AT^TAAT
3206 ATTAAT
ScaI 1315 AGTACT AGT^ACT
2823 AGTACT
XhoI 1193 CTCGAG C^TCGAG
5713 CTCGAG
XmnI 1399 GAAATTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1744 GAAATCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 113 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI AspHI AvaI
AvaII BanI BanII BbsI BbvI
BciVI BcnI BfaI BglI BglII
BmyI BpmI BpuAI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrI BssKI
BstAPI BstEII BstF5I BstNI BstPI
BstXI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraI DraII DsaI
EaeI EarI EcoNI EcoO109I EcoO65I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MspA1I MspI
MvaI MwoI NciI NcoI NdeI
NdeII NlaIII NlaIV NspI PleI
PpuMI PstI PvuII RsaI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XhoII