##################################
# NO CUT ENZYMES ( 95 enzymes) #
##################################
AatII AccII AccIII AcyI AgeI
AhdI AlwNI AocI Aor51HI ApaI
AscI AspEI AvaI AviII BamHI
BanII BbeI BbvCI BcgI BcnI
BglI BlpI Bpu1102I BsaHI BseAI
BsiEI BsiWI BsmBI BsoBI BspDI
BspEI BsrBI BsrFI BssHII BssSI
BstEII BstPI BstUI Bsu36I CelII
CfoI Cfr10I ClaI CpoI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO65I FauI FseI FspI
HaeII HhaI Hin1I HinP1I HpaI
KasI KspI MluI MroI MvnI
NaeI NarI NciI NdeI NgoMIV
NotI NruI PacI PaeR7I PinAI
PmeI PshAI PvuI RsrII SacI
SacII SalI SapI SbfI SfiI
SfoI SgrAI SmaI SnaBI Sse8387I
SwaI XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
Acc65I 398 GGTACC G^GTACC
AciI 1132 CCGC CCGC(-3/-1)
AflII 4265 CTTAAG C^TTAAG
Alw44I 4950 GTGCAC G^TGCAC
ApaLI 4950 GTGCAC G^TGCAC
Asp718I 398 GGTACC G^GTACC
AspI 2349 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 5271 CCTAGG C^CTAGG
BbrPI 705 CACGTG CAC^GTG
BbsI 2226 GTCTTC GAAGAC(2/6)
BclI 1540 TGATCA T^GATCA
BfrI 4265 CTTAAG C^TTAAG
BlnI 5271 CCTAGG C^CTAGG
BpuAI 2226 GTCTTC GAAGAC(2/6)
BsaBI 515 GATCCGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 2034 TCCGGT [AT]^CCGG[AT]
Bst1107I 2302 GTATAC GTA^TAC
BstBI 474 TTCGAA TT^CGAA
BstXI 29 CCAAGACCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 2302 GTATAC GTA^TAC
DraII 3798 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoNI 5190 CCTCAAAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 3798 AGGTCCT [GA]G^G[ACGT]CC[CT]
FbaI 1540 TGATCA T^GATCA
HapII 2035 CCGG C^CGG
HgaI 2939 GACGC GACGC(5/10)
HpaII 2035 CCGG C^CGG
KpnI 398 GGTACC GGTAC^C
MamI 515 GATCCGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspA1I 792 CAGCTG C[AC]G^C[GT]G
MspI 2035 CCGG C^CGG
NspV 474 TTCGAA TT^CGAA
PflFI 2349 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 705 CACGTG CAC^GTG
PmlI 705 CACGTG CAC^GTG
PpuMI 3798 AGGTCCT [GA]G^G[AT]CC[CT]
PstI 3214 CTGCAG CTGCA^G
PvuII 792 CAGCTG CAG^CTG
SexAI 3105 ACCTGGT A^CC[AT]GGT
SfuI 474 TTCGAA TT^CGAA
SpeI 3259 ACTAGT A^CTAGT
SphI 4257 GCATGC GCATG^C
StuI 2237 AGGCCT AGG^CCT
Tth111I 2349 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
BanI 236 GGTGCC G^G[CT][GA]CC
398 GGTACC
BpmI 3170 CTCCAG CTGGAG(16/14)
4758 CTCCAG
BsaI 1128 GAGACC GGTCTC(1/5)
3167 GGTCTC
BspHI 1232 TCATGA T^CATGA
4403 TCATGA
BspMI 233 GCAGGT ACCTGC(4/8)
1625 GCAGGT
BstAPI 428 GCATCTATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1012 GCAAAAGTTGC
DraIII 1199 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
3104 CACCTGGTG
DsaI 35 CCATGG C^C[GA][CT]GG
3074 CCATGG
EcoRI 1752 GAATTC G^AATTC
4365 GAATTC
HincII 1116 GTCAAC GT[CT]^[GA]AC
5143 GTCAAC
HindII 1116 GTCAAC GT[CT]^[GA]AC
5143 GTCAAC
HindIII 242 AAGCTT A^AGCTT
2607 AAGCTT
MfeI 4344 CAATTG C^AATTG
5092 CAATTG
MunI 4344 CAATTG C^AATTG
5092 CAATTG
NcoI 35 CCATGG C^CATGG
3074 CCATGG
NheI 2666 GCTAGC G^CTAGC
4149 GCTAGC
PflMI 2345 CCAAGACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3069 CCATGCCATGG
RcaI 1232 TCATGA T^CATGA
4403 TCATGA
ScaI 3337 AGTACT AGT^ACT
3542 AGTACT
SmlI 949 CTCAAG C^T[CT][GA]AG
4265 CTTAAG
SspI 959 AATATT AAT^ATT
5222 AATATT
TaqI 475 TCGA T^CGA
871 TCGA
TthHB8I 475 TCGA T^CGA
871 TCGA
Van91I 2345 CCAAGACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3069 CCATGCCATGG
XbaI 1069 TCTAGA T^CTAGA
1814 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 94 enzymes) #
#########################################
AccI AclI AcsI AfaI AflIII
AluI AlwI ApoI AseI AsnI
Asp700I AspHI AvaII BalI BbvI
BciVI BfaI BglII BmyI BsaAI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
Bsp1407I BspLU11I BsrDI BsrGI BsrI
BssKI BstF5I BstNI BstYI Cac8I
Cfr13I DdeI DpnII DraI EaeI
EarI EcoRII EcoRV EcoT14I EcoT22I
Fnu4HI FokI HaeIII HinfI HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MvaI MwoI
NdeII NlaIII NlaIV NsiI NspI
PleI PshBI Psp1406I RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SspBI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI XhoII XmnI