##################################
# NO CUT ENZYMES ( 81 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AgeI ApaI AscI AseI AsnI
Asp718I AviII AvrII BalI BanI
BbeI BbrPI BbvCI BglI BlnI
BsaBI BsaHI BseAI BsiEI BsiWI
BspDI BspEI BspHI BsrBI BsrFI
BssHII BstBI Cfr10I ClaI CpoI
DrdI EaeI EagI EclXI Eco52I
FseI FspI Hin1I KasI KpnI
KspI MamI MluI MluNI MroI
MscI NaeI NarI NgoMIV NheI
NotI NruI NspV PacI PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SbfI SexAI SfiI SfoI SfuI
SgrAI SnaBI SphI Sse8387I SwaI
XcmI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AccII 4466 CGCG CG^CG
AflII 1714 CTTAAG C^TTAAG
AocI 3284 CCTGAGG CC^T[ACGT]AGG
AspI 2963 GACTTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 4090 GGATCC G^GATCC
BfrI 1714 CTTAAG C^TTAAG
BsaAI 1151 TACGTG [CT]AC^GT[GA]
BsaWI 3656 ACCGGA [AT]^CCGG[AT]
BseRI 438 GAGGAG GAGGAG(10/8)
BsmBI 5307 CGTCTC CGTCTC(1/5)
Bsp1407I 1924 TGTACA T^GTACA
BspMI 457 GCAGGT ACCTGC(4/8)
BsrGI 1924 TGTACA T^GTACA
BssSI 5260 CACGAG CACGAG(-5/-1)
Bst1107I 5105 GTATAC GTA^TAC
BstUI 4466 CGCG CG^CG
BstZ17I 5105 GTATAC GTA^TAC
Bsu36I 3284 CCTGAGG CC^T[ACGT]AGG
DraIII 486 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 3284 CCTGAGG CC^T[ACGT]AGG
EcoNI 3065 CCTGCTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 207 GAATTC G^AATTC
EcoRV 2081 GATATC GAT^ATC
HgaI 550 GCGTC GACGC(5/10)
HindIII 2147 AAGCTT A^AGCTT
HpaI 2752 GTTAAC GTT^AAC
MfeI 5173 CAATTG C^AATTG
MunI 5173 CAATTG C^AATTG
MvnI 4466 CGCG CG^CG
PaeR7I 369 CTCGAG C^TCGAG
PflFI 2963 GACTTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 3632 CCAGAGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 123 GACATAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 1092 GAGCTC GAGCT^C
SapI 1893 GAAGAGC GCTCTTC(1/4)
SmaI 4017 CCCGGG CCC^GGG
SpeI 3098 ACTAGT A^CTAGT
SspBI 1924 TGTACA T^GTACA
StuI 3224 AGGCCT AGG^CCT
Tth111I 2963 GACTTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 3632 CCAGAGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 369 CTCGAG C^TCGAG
XmaCI 4017 CCCGGG C^CCGGG
XmaI 4017 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AhdI 334 GACATTAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1720 GACTTCCTGTC
Alw44I 483 GTGCAC G^TGCAC
2367 GTGCAC
Aor51HI 2611 AGCGCT AGC^GCT
4203 AGCGCT
ApaLI 483 GTGCAC G^TGCAC
2367 GTGCAC
Asp700I 1419 GAATAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5075 GAATTTCTTC
AspEI 334 GACATTAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1720 GACTTCCTGTC
AvaI 369 CTCGAG C^[CT]CG[GA]G
4017 CCCGGG
BanII 1092 GAGCTC G[GA]GC[CT]^C
1221 GAGCCC
BcgI 3844 CGAACTTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
5383 GCAGGAAAATCG
BsaI 692 GAGACC GGTCTC(1/5)
5277 GAGACC
BsoBI 369 CTCGAG C^[CT]CG[GA]G
4017 CCCGGG
BstAPI 485 GCACTGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
5409 GCAGAGGTTGC
BstEII 460 GGTGACC G^GT[ACGT]ACC
1363 GGTTACC
BstPI 460 GGTGACC G^GT[ACGT]ACC
1363 GGTTACC
Eam1105I 334 GACATTAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1720 GACTTCCTGTC
Eco47III 2611 AGCGCT AGC^GCT
4203 AGCGCT
EcoO65I 460 GGTGACC G^GT[ACGT]ACC
1363 GGTTACC
EcoT22I 3393 ATGCAT ATGCA^T
3712 ATGCAT
FauI 4653 CCCGC CCCGC(4/6)
5250 GCGGG
HaeII 2611 AGCGCT [GA]GCGC^[CT]
4203 AGCGCT
NdeI 1808 CATATG CA^TATG
5097 CATATG
NsiI 3393 ATGCAT ATGCA^T
3712 ATGCAT
ScaI 1697 AGTACT AGT^ACT
1900 AGTACT
SspI 3997 AATATT AAT^ATT
5161 AATATT
XbaI 418 TCTAGA T^CTAGA
3409 TCTAGA
XmnI 1419 GAATAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5075 GAATTTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 107 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI AspHI
AvaII BbsI BbvI BciVI BclI
BcnI BfaI BglII BlpI BmyI
BpmI Bpu1102I BpuAI BsaJI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I BspLU11I BsrDI BsrI
BssKI BstF5I BstNI BstXI BstYI
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraI DraII DsaI EarI
EcoO109I EcoRII EcoT14I FbaI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII