##################################
# NO CUT ENZYMES ( 49 enzymes) #
##################################
AatII AclI AgeI AhdI AscI
AseI AsnI AspEI AviII BcgI
BsiWI BsmI BspHI BssHII BssSI
Bst1107I BstAPI BstBI BstZ17I CpoI
Eam1105I EcoRI EcoRV FspI HindIII
KspI MfeI MluI MunI NdeI
NotI NruI NspV PacI PinAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SapI SfuI SgrAI
SnaBI SpeI SphI SwaI
###################################
# ONE CUT ENZYMES ( 31 enzymes) #
###################################
AcsI 3355 AAATTC [GA]^AATT[CT]
AflII 3781 CTTAAG C^TTAAG
ApaI 4708 GGGCCC GGGCC^C
ApoI 3355 AAATTC [GA]^AATT[CT]
Asp700I 1176 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 4814 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 3466 CCTAGG C^CTAGG
BbrPI 3546 CACGTG CAC^GTG
BfrI 3781 CTTAAG C^TTAAG
BlnI 3466 CCTAGG C^CTAGG
BsaBI 1551 GATCAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BspDI 5550 ATCGAT AT^CGAT
ClaI 5550 ATCGAT AT^CGAT
DraI 4541 TTTAAA TTT^AAA
DraIII 5515 CACCCGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 3623 GACCAAAGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FseI 2767 GGCCGGCC GGCCGG^CC
HpaI 3978 GTTAAC GTT^AAC
MamI 1551 GATCAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
PflFI 4814 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 3546 CACGTG CAC^GTG
PmeI 4540 GTTTAAAC GTTT^AAAC
PmlI 3546 CACGTG CAC^GTG
PshAI 2129 GACAGATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SbfI 4702 CCTGCAGG CCTGCA^GG
ScaI 8 AGTACT AGT^ACT
SexAI 1106 ACCTGGT A^CC[AT]GGT
SfiI 2287 GGCCACATAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Sse8387I 4702 CCTGCAGG CCTGCA^GG
Tth111I 4814 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 1176 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
Acc65I 2380 GGTACC G^GTACC
5028 GGTACC
AccI 2104 GTCTAC GT^[AC][GT]AC
3376 GTAGAC
AflIII 1998 ACATGT A^C[GA][CT]GT
3590 ACATGT
Aor51HI 1385 AGCGCT AGC^GCT
1890 AGCGCT
Asp718I 2380 GGTACC G^GTACC
5028 GGTACC
BclI 2166 TGATCA T^GATCA
4727 TGATCA
BglII 549 AGATCT A^GATCT
938 AGATCT
BsaAI 67 TACGTG [CT]AC^GT[GA]
3546 CACGTG
BsaI 1805 GAGACC GGTCTC(1/5)
5609 GAGACC
Bsp1407I 2001 TGTACA T^GTACA
5234 TGTACA
BspLU11I 1998 ACATGT A^CATGT
3590 ACATGT
BsrGI 2001 TGTACA T^GTACA
5234 TGTACA
EagI 1045 CGGCCG C^GGCCG
1520 CGGCCG
EarI 5494 CTCTTC CTCTTC(1/4)
1167 GAAGAG
EclXI 1045 CGGCCG C^GGCCG
1520 CGGCCG
Eco47III 1385 AGCGCT AGC^GCT
1890 AGCGCT
Eco52I 1045 CGGCCG C^GGCCG
1520 CGGCCG
FbaI 2166 TGATCA T^GATCA
4727 TGATCA
HincII 26 GTCAAC GT[CT]^[GA]AC
3978 GTTAAC
HindII 26 GTCAAC GT[CT]^[GA]AC
3978 GTTAAC
KpnI 2380 GGTACC GGTAC^C
5028 GGTACC
Ksp632I 5494 CTCTTC CTCTTC(1/4)
1167 GAAGAG
NheI 210 GCTAGC G^CTAGC
2648 GCTAGC
PaeR7I 205 CTCGAG C^TCGAG
227 CTCGAG
SmaI 2098 CCCGGG CCC^GGG
5219 CCCGGG
SspBI 2001 TGTACA T^GTACA
5234 TGTACA
XhoI 205 CTCGAG C^TCGAG
227 CTCGAG
XmaCI 2098 CCCGGG C^CCGGG
5219 CCCGGG
XmaI 2098 CCCGGG C^CCGGG
5219 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 149 enzymes) #
#########################################
AccII AccIII AciI AcyI AfaI
AluI Alw44I AlwI AlwNI AocI
ApaLI AspHI AvaI AvaII BalI
BamHI BanI BanII BbeI BbsI
BbvCI BbvI BciVI BcnI BfaI
BglI BlpI BmyI BpmI Bpu1102I
BpuAI BsaHI BsaJI BsaWI BseAI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
Bsp1286I BspEI BspMI BsrBI BsrDI
BsrFI BsrI BssKI BstEII BstF5I
BstNI BstPI BstUI BstXI BstYI
Bsu36I Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI Eco81I EcoNI EcoO109I EcoO65I
EcoRII EcoT14I EcoT22I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI KasI MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MroI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NarI NciI NcoI NdeII
NgoMIV NlaIII NlaIV NsiI NspI
PflMI PleI PpuMI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SfoI SmlI SspI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XbaI XcmI XhoII