##################################
# NO CUT ENZYMES (   72 enzymes) #
##################################

AatII       Acc65I      AccI        AclI        AflII       
AflIII      AgeI        Alw44I      ApaLI       Asp700I     
Asp718I     AviII       AvrII       BamHI       BbsI        
BciVI       BclI        BfrI        BlnI        BpuAI       
BsiWI       BsmI        Bsp1407I    BspDI       BspHI       
BspLU11I    BsrGI       BssSI       Bst1107I    BstBI       
BstEII      BstPI       BstZ17I     ClaI        CpoI        
EcoO65I     EcoRI       EcoRV       EcoT22I     FbaI        
FseI        FspI        HindIII     HpaI        KpnI        
MfeI        MluI        MunI        NcoI        NdeI        
NotI        NruI        NsiI        NspV        PacI        
PinAI       PmeI        Psp1406I    PvuI        RcaI        
RsrII       SalI        SexAI       SfuI        SgrAI       
SnaBI       SpeI        SphI        SspBI       SwaI        
XbaI        XmnI        


###################################
# ONE CUT ENZYMES (   22 enzymes) #
###################################

AcsI         4589  AAATTT               [GA]^AATT[CT]
Aor51HI      2021  AGCGCT               AGC^GCT
ApoI         4589  AAATTT               [GA]^AATT[CT]
AscI         3163  GGCGCGCC             GG^CGCGCC
AseI         3901  ATTAAT               AT^TAAT
AsnI         3901  ATTAAT               AT^TAAT
BglII         290  AGATCT               A^GATCT
BlpI         2364  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     2364  GCTCAGC              GC^T[ACGT]AGC
BsaI         4525  GGTCTC               GGTCTC(1/5)
BsmBI         438  CGTCTC               CGTCTC(1/5)
CelII        2364  GCTCAGC              GC^T[ACGT]AGC
EarI         2172  GAAGAG               CTCTTC(1/4)
Eco47III     2021  AGCGCT               AGC^GCT
HincII       1957  GTCAAC               GT[CT]^[GA]AC
HindII       1957  GTCAAC               GT[CT]^[GA]AC
Ksp632I      2172  GAAGAG               CTCTTC(1/4)
NheI         4117  GCTAGC               G^CTAGC
PshAI        3843  GACCAAGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI        3901  ATTAAT               AT^TAAT
SapI         2172  GAAGAGC              GCTCTTC(1/4)
SspI         3986  AATATT               AAT^ATT


###################################
# TWO CUT ENZYMES (   28 enzymes) #
###################################

AhdI         1096  GACTGCCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             4027  GACACGAAGTC         
AlwI         3339  GATCC                GGATC(4/5)
             4547  GATCC               
AspEI        1096  GACTGCCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             4027  GACACGAAGTC         
AspI         2595  GACCGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2605  GACACGGTC           
BbrPI        3178  CACGTG               CAC^GTG
             4312  CACGTG              
BsaBI         918  GATCGGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1053  GATGGCCATC          
BsrDI        2798  CATTGC               GCAATG(2/0)
              158  GCAATG              
BssHII       2952  GCGCGC               G^CGCGC
             3164  GCGCGC              
BstYI         290  AGATCT               [GA]^GATC[CT]
             3338  AGATCC              
DraI         3974  TTTAAA               TTT^AAA
             4592  TTTAAA              
DrdI         1547  GACCTCATGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1948  GACAAGAACGTC        
Eam1105I     1096  GACTGCCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             4027  GACACGAAGTC         
MamI          918  GATCGGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1053  GATGGCCATC          
MflI          290  AGATCT               [GA]^GATC[CT]
             3338  AGATCC              
MslI          112  CATCCGGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             4411  CACCCCAGTG          
NspI         3419  GCATGT               [GA]CATG^[CT]
             4326  GCATGT              
PaeR7I        103  CTCGAG               C^TCGAG
             4208  CTCGAG              
PflFI        2595  GACCGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2605  GACACGGTC           
PmaCI        3178  CACGTG               CAC^GTG
             4312  CACGTG              
PmlI         3178  CACGTG               CAC^GTG
             4312  CACGTG              
SacI          585  GAGCTC               GAGCT^C
             1135  GAGCTC              
SbfI           18  CCTGCAGG             CCTGCA^GG
              909  CCTGCAGG            
ScaI          839  AGTACT               AGT^ACT
             4515  AGTACT              
Sse8387I       18  CCTGCAGG             CCTGCA^GG
              909  CCTGCAGG            
TfiI         1818  GAATC                G^A[AT]TC
             3995  GATTC               
Tth111I      2595  GACCGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2605  GACACGGTC           
XhoI          103  CTCGAG               C^TCGAG
             4208  CTCGAG              
XhoII         290  AGATCT               [GA]^GATC[CT]
             3338  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (  136 enzymes) #
#########################################

AccII       AccIII      AciI        AcyI        AfaI        
AluI        AlwNI       AocI        ApaI        AspHI       
AvaI        AvaII       BalI        BanI        BanII       
BbeI        BbvCI       BbvI        BcgI        BcnI        
BfaI        BglI        BmyI        BpmI        BsaAI       
BsaHI       BsaJI       BsaWI       BseAI       BseRI       
BsgI        BsiEI       BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       BsoBI       Bsp1286I    BspEI       
BspMI       BsrBI       BsrFI       BsrI        BssKI       
BstAPI      BstF5I      BstNI       BstUI       BstXI       
Bsu36I      Cac8I       CfoI        Cfr10I      Cfr13I      
DdeI        DpnII       DraII       DraIII      DsaI        
EaeI        EagI        EclXI       Eco52I      Eco81I      
EcoNI       EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HgaI        HhaI        Hin1I       HinP1I      HinfI       
HpaII       HphI        ItaI        KasI        KspI        
MaeI        MaeII       MaeIII      MboI        MboII       
MluNI       MnlI        MroI        MscI        MseI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NaeI        NarI        NciI        NdeII       NgoMIV      
NlaIII      NlaIV       PflMI       PleI        PpuMI       
PstI        PvuII       RsaI        SacII       Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SfiI        
SfoI        SmaI        SmlI        StuI        StyI        
TaqI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     Van91I      XcmI        XmaCI       
XmaI