##################################
# NO CUT ENZYMES ( 97 enzymes) #
##################################
AatII AccIII AclI AcyI AgeI
AhdI AocI Aor51HI ApaI AscI
AspEI AvaI AviII BamHI BbeI
BbrPI BcgI BglII BsaAI BsaHI
BseAI BsiEI BsiWI BsoBI Bsp1407I
BspDI BspEI BspHI BsrFI BsrGI
BssHII BssSI BstBI Bsu36I Cfr10I
ClaI CpoI DrdI DsaI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoRV EcoT22I FseI FspI
Hin1I HpaI KasI KspI MluI
MroI NaeI NarI NcoI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshAI Psp1406I PvuI RcaI
RsrII SacII SalI SapI SbfI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SspBI
StuI TaqI TthHB8I XbaI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
Acc65I 2047 GGTACC G^GTACC
AccII 37 CGCG CG^CG
AflII 4365 CTTAAG C^TTAAG
Alw44I 703 GTGCAC G^TGCAC
ApaLI 703 GTGCAC G^TGCAC
Asp700I 1716 GAACATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2047 GGTACC G^GTACC
AvrII 3879 CCTAGG C^CTAGG
BanII 2671 GAGCTC G[GA]GC[CT]^C
BciVI 1398 GTATCC GTATCC(6/5)
BclI 2022 TGATCA T^GATCA
BcnI 1392 CCGGG CC^[GC]GG
BfrI 4365 CTTAAG C^TTAAG
BglI 179 GCCACCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 3879 CCTAGG C^CTAGG
BlpI 2037 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 2037 GCTAAGC GC^T[ACGT]AGC
BsaI 1191 GAGACC GGTCTC(1/5)
BseRI 2534 GAGGAG GAGGAG(10/8)
BsmBI 33 GAGACG CGTCTC(1/5)
BsmI 1084 GCATTC GAATGC(1/-1)
BspMI 1321 ACCTGC ACCTGC(4/8)
BsrBI 40 GAGCGG CCGCTC(-3/-3)
Bst1107I 3546 GTATAC GTA^TAC
BstAPI 616 GCAGAATGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1293 GGTTACC G^GT[ACGT]ACC
BstPI 1293 GGTTACC G^GT[ACGT]ACC
BstUI 37 CGCG CG^CG
BstXI 2763 CCAGGACAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 3546 GTATAC GTA^TAC
CelII 2037 GCTAAGC GC^T[ACGT]AGC
EcoO65I 1293 GGTTACC G^GT[ACGT]ACC
EcoRI 679 GAATTC G^AATTC
FauI 104 CCCGC CCCGC(4/6)
FbaI 2022 TGATCA T^GATCA
HaeII 187 GGCGCT [GA]GCGC^[CT]
HgaI 35 GACGC GACGC(5/10)
HincII 3719 GTCAAC GT[CT]^[GA]AC
HindII 3719 GTCAAC GT[CT]^[GA]AC
HindIII 1513 AAGCTT A^AGCTT
KpnI 2047 GGTACC GGTAC^C
MfeI 3517 CAATTG C^AATTG
MspA1I 1877 CAGCTG C[AC]G^C[GT]G
MunI 3517 CAATTG C^AATTG
MvnI 37 CGCG CG^CG
NciI 1392 CCGGG CC^[GC]GG
PvuII 1877 CAGCTG CAG^CTG
SacI 2671 GAGCTC GAGCT^C
SphI 3592 GCATGC GCATG^C
XcmI 2196 CCATATGAGGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 1716 GAACATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccI 3546 GTATAC GT^[AC][GT]AC
4713 GTAGAC
AciI 105 CCGC CCGC(-3/-1)
42 GCGG
AflIII 1718 ACATGT A^C[GA][CT]GT
4281 ACATGT
BalI 2090 TGGCCA TGG^CCA
2469 TGGCCA
BanI 1445 GGTGCC G^G[CT][GA]CC
2047 GGTACC
BbvCI 1471 CCTCAGC CCTCAGC(-5/-2)
1840 GCTGAGG
BsaBI 3182 GATTTAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4251 GATAATTATC
BsaWI 2371 ACCGGA [AT]^CCGG[AT]
2744 ACCGGA
BspLU11I 1718 ACATGT A^CATGT
4281 ACATGT
BsrDI 2949 CATTGC GCAATG(2/0)
1565 GCAATG
CfoI 188 GCGC GCG^C
1572 GCGC
DraIII 4100 CACTATGTG CAC[ACGT][ACGT][ACGT]^GTG
4692 CACCATGTG
EaeI 2090 TGGCCA [CT]^GGCC[GA]
2469 TGGCCA
HhaI 188 GCGC GCG^C
1572 GCGC
HinP1I 188 GCGC G^CGC
1572 GCGC
MamI 3182 GATTTAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4251 GATAATTATC
MluNI 2090 TGGCCA TGG^CCA
2469 TGGCCA
MscI 2090 TGGCCA TGG^CCA
2469 TGGCCA
NdeI 2197 CATATG CA^TATG
2453 CATATG
PflMI 1199 CCATTATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2127 CCAACCAGTGG
ScaI 566 AGTACT AGT^ACT
3989 AGTACT
SwaI 3183 ATTTAAAT ATTT^AAAT
4009 ATTTAAAT
Van91I 1199 CCATTATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2127 CCAACCAGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 87 enzymes) #
#########################################
AcsI AfaI AluI AlwI AlwNI
ApoI AseI AsnI AspHI AspI
AvaII BbsI BbvI BfaI BmyI
BpmI BpuAI BsaJI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII DraI
DraII EarI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspI MvaI
MwoI NdeII NlaIII NlaIV NspI
PflFI PleI PpuMI PshBI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspI StyI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Tth111I XhoII