##################################
# NO CUT ENZYMES (   56 enzymes) #
##################################

AatII       AccIII      AclI        AflII       AgeI        
AocI        Aor51HI     AscI        AviII       AvrII       
BcgI        BfrI        BlnI        BsaWI       BseAI       
BsiEI       BsiWI       BsmBI       BspDI       BspEI       
BssHII      Bsu36I      ClaI        DraIII      EagI        
EclXI       Eco47III    Eco52I      Eco81I      FseI        
FspI        MluI        MroI        NcoI        NotI        
NruI        PacI        PflMI       PinAI       PmeI        
PshAI       Psp1406I    PvuI        SalI        SbfI        
ScaI        SfiI        SgrAI       SmaI        SnaBI       
SpeI        Sse8387I    Van91I      XbaI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   65 enzymes) #
###################################

Acc65I       1827  GGTACC               G^GTACC
AhdI           85  GACGCGGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I       5663  GTGCAC               G^TGCAC
ApaI         4375  GGGCCC               GGGCC^C
ApaLI        5663  GTGCAC               G^TGCAC
Asp718I      1827  GGTACC               G^GTACC
AspEI          85  GACGCGGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI         1532  GACAACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI         4228  CTCGAG               C^[CT]CG[GA]G
BalI         1097  TGGCCA               TGG^CCA
BamHI        4304  GGATCC               G^GATCC
BbrPI        4315  CACGTG               CAC^GTG
BciVI        2313  GGATAC               GTATCC(6/5)
BglI         5208  GCCCACAAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII        1676  AGATCT               A^GATCT
BlpI         3961  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     3961  GCTGAGC              GC^T[ACGT]AGC
BsaBI        2741  GATCTGTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmI         4103  GAATGC               GAATGC(1/-1)
BsoBI        4228  CTCGAG               C^[CT]CG[GA]G
Bsp1407I     5901  TGTACA               T^GTACA
BspHI        3726  TCATGA               T^CATGA
BsrGI        5901  TGTACA               T^GTACA
BssSI        5660  CTCGTG               CACGAG(-5/-1)
Bst1107I      776  GTATAC               GTA^TAC
BstBI        5057  TTCGAA               TT^CGAA
BstEII       3111  GGTGACC              G^GT[ACGT]ACC
BstPI        3111  GGTGACC              G^GT[ACGT]ACC
BstZ17I       776  GTATAC               GTA^TAC
CelII        3961  GCTGAGC              GC^T[ACGT]AGC
CpoI         4289  CGGACCG              CG^G[AT]CCG
DrdI         5729  GACAATATAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I       85  GACGCGGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I      3111  GGTGACC              G^GT[ACGT]ACC
EcoRI        1556  GAATTC               G^AATTC
EcoRV         947  GATATC               GAT^ATC
EcoT22I      5871  ATGCAT               ATGCA^T
HpaI         3847  GTTAAC               GTT^AAC
KpnI         1827  GGTACC               GGTAC^C
KspI         4090  CCGCGG               CCGC^GG
MamI         2741  GATCTGTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI         3698  CAATTG               C^AATTG
MluNI        1097  TGGCCA               TGG^CCA
MscI         1097  TGGCCA               TGG^CCA
MunI         3698  CAATTG               C^AATTG
NaeI          327  GCCGGC               GCC^GGC
NgoMIV        327  GCCGGC               G^CCGGC
NsiI         5871  ATGCAT               ATGCA^T
NspV         5057  TTCGAA               TT^CGAA
PaeR7I       4228  CTCGAG               C^TCGAG
PflFI        1532  GACAACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI        4315  CACGTG               CAC^GTG
PmlI         4315  CACGTG               CAC^GTG
RcaI         3726  TCATGA               T^CATGA
RsrII        4289  CGGACCG              CG^G[AT]CCG
SacII        4090  CCGCGG               CCGC^GG
SapI         3745  GAAGAGC              GCTCTTC(1/4)
SexAI        2463  ACCTGGT              A^CC[AT]GGT
SfuI         5057  TTCGAA               TT^CGAA
SphI          527  GCATGC               GCATG^C
SspBI        5901  TGTACA               T^GTACA
SwaI         5764  ATTTAAAT             ATTT^AAAT
Tth111I      1532  GACAACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XcmI         3649  CCAAGTCAACAGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI         4228  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AflIII        206  ACATGT               A^C[GA][CT]GT
             5404  ACATGT              
Asp700I      2260  GAAAAAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             4509  GAAACTTTTC          
BbeI          125  GGCGCC               GGCGC^C
             4790  GGCGCC              
BclI          291  TGATCA               T^GATCA
             5687  TGATCA              
BsaAI         299  CACGTA               [CT]AC^GT[GA]
             4315  CACGTG              
BspLU11I      206  ACATGT               A^CATGT
             5404  ACATGT              
BspMI        3453  ACCTGC               ACCTGC(4/8)
             5144  ACCTGC              
BstAPI       3271  GCATATGTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             5335  GCAATCTATGC         
DraI         4068  TTTAAA               TTT^AAA
             5765  TTTAAA              
DsaI         4090  CCGCGG               C^C[GA][CT]GG
             5521  CCGTGG              
EaeI         1097  TGGCCA               [CT]^GGCC[GA]
             3956  TGGCCG              
EcoT14I      3530  CCAAGG               C^C[AT][AT]GG
             5431  CCAAGG              
FbaI          291  TGATCA               T^GATCA
             5687  TGATCA              
HaeII         125  GGCGCC               [GA]GCGC^[CT]
             4790  GGCGCC              
HincII       3653  GTCAAC               GT[CT]^[GA]AC
             3847  GTTAAC              
HindII       3653  GTCAAC               GT[CT]^[GA]AC
             3847  GTTAAC              
KasI          125  GGCGCC               G^GCGCC
             4790  GGCGCC              
NarI          125  GGCGCC               GG^CGCC
             4790  GGCGCC              
PpuMI         865  GGGACCT              [GA]G^G[AT]CC[CT]
             5111  AGGTCCC             
SacI         3517  GAGCTC               GAGCT^C
             3859  GAGCTC              
SfoI          125  GGCGCC               GGC^GCC
             4790  GGCGCC              
StuI         2570  AGGCCT               AGG^CCT
             4329  AGGCCT              
StyI         3530  CCAAGG               C^C[AT][AT]GG
             5431  CCAAGG              
XmnI         2260  GAAAAAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             4509  GAAACTTTTC          


#########################################
# 3 OR MORE CUT ENZYMES (  113 enzymes) #
#########################################

AccI        AccII       AciI        AcsI        AcyI        
AfaI        AluI        AlwI        AlwNI       ApoI        
AseI        AsnI        AspHI       AvaII       BanI        
BanII       BbsI        BbvCI       BbvI        BcnI        
BfaI        BmyI        BpmI        BpuAI       BsaHI       
BsaI        BsaJI       BseRI       BsgI        BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       Bsp1286I    
BsrBI       BsrDI       BsrFI       BsrI        BssKI       
BstF5I      BstNI       BstUI       BstXI       BstYI       
Cac8I       CfoI        Cfr10I      Cfr13I      DdeI        
DpnII       DraII       EarI        EcoNI       EcoO109I    
EcoRII      FauI        Fnu4HI      FokI        HaeIII      
HapII       HgaI        HhaI        Hin1I       HinP1I      
HindIII     HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeII       MaeIII      MboI        
MboII       MflI        MnlI        MseI        MslI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NciI        NdeI        NdeII       NheI        NlaIII      
NlaIV       NspI        PleI        PshBI       PstI        
PvuII       RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SmlI        SspI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     XhoII