##################################
# NO CUT ENZYMES ( 62 enzymes) #
##################################
AccIII AgeI Aor51HI AscI AviII
BalI BbeI BsaBI BseAI BsiWI
BsmBI BspDI BspEI BssHII BssSI
BstBI BstEII BstPI ClaI CpoI
DrdI EaeI EagI EclXI Eco47III
Eco52I EcoO65I EcoRV FseI FspI
HpaI KasI MamI MfeI MluI
MluNI MroI MscI MunI NaeI
NarI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PinAI PmeI
RsrII SalI SbfI SfiI SfoI
SfuI SgrAI SnaBI Sse8387I SwaI
XbaI XhoI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
AatII 5843 GACGTC GACGT^C
Acc65I 2824 GGTACC G^GTACC
AcyI 5843 GACGTC G[GA]^CG[CT]C
AflII 1995 CTTAAG C^TTAAG
AflIII 4491 ACATGT A^C[GA][CT]GT
AhdI 701 GACGGTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 4641 GGGCCC GGGCC^C
AseI 1581 ATTAAT AT^TAAT
AsnI 1581 ATTAAT AT^TAAT
Asp718I 2824 GGTACC G^GTACC
AspEI 701 GACGGTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 983 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 2796 GGATCC G^GATCC
BbvCI 3135 GCTGAGG CCTCAGC(-5/-2)
BclI 4666 TGATCA T^GATCA
BfrI 1995 CTTAAG C^TTAAG
BglII 512 AGATCT A^GATCT
BsaHI 5843 GACGTC G[GA]^CG[CT]C
BsaWI 5096 TCCGGT [AT]^CCGG[AT]
BsiEI 1249 CGATCG CG[GA][CT]^CG
Bsp1407I 1139 TGTACA T^GTACA
BspHI 1960 TCATGA T^CATGA
BspLU11I 4491 ACATGT A^CATGT
BsrFI 4898 ACCGGC [GA]^CCGG[CT]
BsrGI 1139 TGTACA T^GTACA
Bst1107I 6872 GTATAC GTA^TAC
BstZ17I 6872 GTATAC GTA^TAC
Cfr10I 4898 ACCGGC [GA]^CCGG[CT]
DraIII 1231 CACCACGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 701 GACGGTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 4553 CCTGGATCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 4666 TGATCA T^GATCA
HaeII 4301 AGCGCC [GA]GCGC^[CT]
Hin1I 5843 GACGTC G[GA]^CG[CT]C
KpnI 2824 GGTACC GGTAC^C
KspI 3427 CCGCGG CCGC^GG
PflFI 983 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 880 GACTTACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1581 ATTAAT AT^TAAT
PvuI 1249 CGATCG CGAT^CG
RcaI 1960 TCATGA T^CATGA
SacII 3427 CCGCGG CCGC^GG
ScaI 5194 AGTACT AGT^ACT
SexAI 4130 ACCTGGT A^CC[AT]GGT
SmaI 5370 CCCGGG CCC^GGG
SpeI 2327 ACTAGT A^CTAGT
SspBI 1139 TGTACA T^GTACA
SspI 6943 AATATT AAT^ATT
StuI 6274 AGGCCT AGG^CCT
Tth111I 983 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 5370 CCCGGG C^CCGGG
XmaI 5370 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccI 1772 GTCTAC GT^[AC][GT]AC
6872 GTATAC
AccII 3029 CGCG CG^CG
3428 CGCG
AclI 1243 AACGTT AA^CGTT
2957 AACGTT
Alw44I 405 GTGCAC G^TGCAC
6362 GTGCAC
ApaLI 405 GTGCAC G^TGCAC
6362 GTGCAC
Asp700I 279 GAAAGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5919 GAAAACATTC
AvrII 3452 CCTAGG C^CTAGG
6241 CCTAGG
BcgI 682 CGAGAACGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
795 GCAGTGGAATCG
BglI 2705 GCCTTCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3361 GCCCATTTGGC
BlnI 3452 CCTAGG C^CTAGG
6241 CCTAGG
BlpI 1518 GCTCAGC GC^T[ACGT]AGC
4596 GCTCAGC
Bpu1102I 1518 GCTCAGC GC^T[ACGT]AGC
4596 GCTCAGC
BsaI 3378 GGTCTC GGTCTC(1/5)
5469 GGTCTC
BsrBI 179 GAGCGG CCGCTC(-3/-3)
3178 GAGCGG
BsrDI 3830 CATTGC GCAATG(2/0)
4464 GCAATG
BstAPI 3184 GCAGCTTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3352 GCATGAGTTGC
BstUI 3029 CGCG CG^CG
3428 CGCG
CelII 1518 GCTCAGC GC^T[ACGT]AGC
4596 GCTCAGC
EcoRI 846 GAATTC G^AATTC
1220 GAATTC
HgaI 3077 GACGC GACGC(5/10)
2662 GCGTC
MvnI 3029 CGCG CG^CG
3428 CGCG
NdeI 4382 CATATG CA^TATG
5028 CATATG
Psp1406I 1243 AACGTT AA^CGTT
2957 AACGTT
SphI 3939 GCATGC GCATG^C
5754 GCATGC
XcmI 510 CCAGATCTCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
6098 CCAGGAGGCGTTTGG
XmnI 279 GAAAGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5919 GAAAACATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 118 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI AocI ApoI AspHI AvaI
AvaII BanI BanII BbrPI BbsI
BbvI BciVI BcnI BfaI BmyI
BpmI BpuAI BsaAI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI BstXI
BstYI Bsu36I Cac8I CfoI Cfr13I
DdeI DpnII DraI DraII DsaI
EarI Eco81I EcoO109I EcoRII EcoT14I
EcoT22I FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HincII HindII
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MwoI NciI
NcoI NdeII NlaIII NlaIV NsiI
NspI PflMI PleI PmaCI PmlI
PpuMI PstI PvuII RsaI SacI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XhoII