##################################
# NO CUT ENZYMES ( 83 enzymes) #
##################################
Acc65I AccIII AgeI AhdI Aor51HI
ApaI AscI Asp700I Asp718I AspEI
AviII BbeI BcgI BglI BglII
BlpI Bpu1102I BsaBI BsaWI BseAI
BsiEI BsiWI BsmBI BspDI BspEI
BsrBI BsrFI BssHII Bst1107I BstBI
BstEII BstPI BstZ17I CelII Cfr10I
ClaI CpoI DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoO65I FauI
FseI FspI HaeII HgaI HindIII
KasI KpnI KspI MamI MluI
MroI NaeI NarI NgoMIV NotI
NruI NspV PacI PaeR7I PinAI
PpuMI PshAI PvuI RsrII SacII
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SnaBI SpeI Sse8387I
SwaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AatII 1612 GACGTC GACGT^C
AccII 940 CGCG CG^CG
AclI 3913 AACGTT AA^CGTT
AcyI 1612 GACGTC G[GA]^CG[CT]C
AflII 4980 CTTAAG C^TTAAG
Alw44I 1520 GTGCAC G^TGCAC
ApaLI 1520 GTGCAC G^TGCAC
AseI 4308 ATTAAT AT^TAAT
AsnI 4308 ATTAAT AT^TAAT
AvrII 3373 CCTAGG C^CTAGG
BamHI 5882 GGATCC G^GATCC
BbrPI 1501 CACGTG CAC^GTG
BfrI 4980 CTTAAG C^TTAAG
BlnI 3373 CCTAGG C^CTAGG
BsaAI 1501 CACGTG [CT]AC^GT[GA]
BsaHI 1612 GACGTC G[GA]^CG[CT]C
Bsp1407I 4513 TGTACA T^GTACA
BspMI 1441 GCAGGT ACCTGC(4/8)
BsrGI 4513 TGTACA T^GTACA
BssSI 1414 CTCGTG CACGAG(-5/-1)
BstUI 940 CGCG CG^CG
DraIII 2163 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoRI 3362 GAATTC G^AATTC
Hin1I 1612 GACGTC G[GA]^CG[CT]C
HpaI 5639 GTTAAC GTT^AAC
MfeI 1154 CAATTG C^AATTG
MunI 1154 CAATTG C^AATTG
MvnI 940 CGCG CG^CG
NdeI 2369 CATATG CA^TATG
NheI 3420 GCTAGC G^CTAGC
PmaCI 1501 CACGTG CAC^GTG
PmeI 5628 GTTTAAAC GTTT^AAAC
PmlI 1501 CACGTG CAC^GTG
PshBI 4308 ATTAAT AT^TAAT
Psp1406I 3913 AACGTT AA^CGTT
SalI 2155 GTCGAC G^TCGAC
SphI 2103 GCATGC GCATG^C
SspBI 4513 TGTACA T^GTACA
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AflIII 3002 ACATGT A^C[GA][CT]GT
5332 ACATGT
AlwNI 268 CAGGCACTG CAG[ACGT][ACGT][ACGT]^CTG
1403 CAGTGGCTG
AvaI 4025 CCCGGG C^[CT]CG[GA]G
4223 CCCGGG
AvaII 308 GGACC G^G[AT]CC
1311 GGACC
BalI 869 TGGCCA TGG^CCA
1890 TGGCCA
BanI 11 GGTGCC G^G[CT][GA]CC
1578 GGTGCC
BclI 1752 TGATCA T^GATCA
4958 TGATCA
BsmI 227 GAATGC GAATGC(1/-1)
1030 GAATGC
BsoBI 4025 CCCGGG C^[CT]CG[GA]G
4223 CCCGGG
BspLU11I 3002 ACATGT A^CATGT
5332 ACATGT
BsrDI 4260 CATTGC GCAATG(2/0)
106 GCAATG
BstAPI 1402 GCAGTGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3518 GCAGGAAATGC
EaeI 869 TGGCCA [CT]^GGCC[GA]
1890 TGGCCA
EcoNI 615 CCTCAATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
5617 CCTCAAATAGG
EcoRV 1904 GATATC GAT^ATC
4872 GATATC
FbaI 1752 TGATCA T^GATCA
4958 TGATCA
MluNI 869 TGGCCA TGG^CCA
1890 TGGCCA
MscI 869 TGGCCA TGG^CCA
1890 TGGCCA
MspA1I 1944 CAGCTG C[AC]G^C[GT]G
3118 CAGCTG
PflMI 1975 CCAGAGGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5254 CCACATGCTGG
PvuII 1944 CAGCTG CAG^CTG
3118 CAGCTG
SacI 187 GAGCTC GAGCT^C
2553 GAGCTC
SmaI 4025 CCCGGG CCC^GGG
4223 CCCGGG
StuI 2097 AGGCCT AGG^CCT
4583 AGGCCT
TaqI 1616 TCGA T^CGA
2156 TCGA
TthHB8I 1616 TCGA T^CGA
2156 TCGA
Van91I 1975 CCAGAGGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5254 CCACATGCTGG
XbaI 954 TCTAGA T^CTAGA
2403 TCTAGA
XmaCI 4025 CCCGGG C^CCGGG
4223 CCCGGG
XmaI 4025 CCCGGG C^CCGGG
4223 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 107 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AocI ApoI AspHI AspI
BanII BbsI BbvCI BbvI BciVI
BcnI BfaI BmyI BpmI BpuAI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspHI BsrI BssKI BstF5I BstNI
BstXI BstYI Bsu36I Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
DsaI EarI Eco81I EcoO109I EcoRII
EcoT14I EcoT22I Fnu4HI FokI HaeIII
HapII HhaI HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspI
MvaI MwoI NciI NcoI NdeII
NlaIII NlaIV NsiI NspI PflFI
PleI PstI RcaI RsaI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Tth111I
XcmI XhoII