##################################
# NO CUT ENZYMES (   69 enzymes) #
##################################

Acc65I      AccI        AccIII      AflIII      Aor51HI     
ApaI        AscI        Asp718I     AviII       BamHI       
BbeI        BclI        BglI        BseAI       BsiEI       
BsiWI       BspDI       BspEI       BspLU11I    BsrBI       
BssHII      BssSI       Bst1107I    BstEII      BstPI       
BstZ17I     ClaI        CpoI        DraII       DrdI        
EagI        EclXI       Eco47III    Eco52I      EcoO109I    
EcoO65I     FauI        FbaI        FseI        FspI        
HindIII     KasI        KpnI        KspI        MluI        
MroI        NaeI        NarI        NgoMIV      NheI        
NotI        NruI        PacI        PmeI        PpuMI       
PvuI        RsrII       SacII       SalI        SbfI        
SexAI       SfiI        SfoI        SgrAI       SnaBI       
Sse8387I    StuI        XbaI        XcmI        


###################################
# ONE CUT ENZYMES (   59 enzymes) #
###################################

AatII        2020  GACGTC               GACGT^C
AccII        1932  CGCG                 CG^CG
AclI         2468  AACGTT               AA^CGTT
AflII        2786  CTTAAG               C^TTAAG
AgeI         2335  ACCGGT               A^CCGGT
AhdI         2015  GACAAGACGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I       4254  GTGCAC               G^TGCAC
ApaLI        4254  GTGCAC               G^TGCAC
AseI         3423  ATTAAT               AT^TAAT
AsnI         3423  ATTAAT               AT^TAAT
AspEI        2015  GACAAGACGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI         3988  GACAGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvrII        2513  CCTAGG               C^CTAGG
BanI          815  GGTGCC               G^G[CT][GA]CC
BbrPI        3537  CACGTG               CAC^GTG
BbvCI        2880  CCTCAGC              CCTCAGC(-5/-2)
BcgI           91  CGAGAGGCATGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI         2786  CTTAAG               C^TTAAG
BlnI         2513  CCTAGG               C^CTAGG
BsaAI        3537  CACGTG               [CT]AC^GT[GA]
BsaI         1514  GAGACC               GGTCTC(1/5)
BseRI         804  GAGGAG               GAGGAG(10/8)
BsmBI        4149  GAGACG               CGTCTC(1/5)
BsrFI        2335  ACCGGT               [GA]^CCGG[CT]
BstBI         204  TTCGAA               TT^CGAA
BstUI        1932  CGCG                 CG^CG
Cfr10I       2335  ACCGGT               [GA]^CCGG[CT]
DraIII       2030  CACAAAGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I     2015  GACAAGACGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI        1075  CCTTTTTGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV        2273  GATATC               GAT^ATC
EcoT22I      2666  ATGCAT               ATGCA^T
HaeII           2  AGCGCC               [GA]GCGC^[CT]
MfeI         1820  CAATTG               C^AATTG
MunI         1820  CAATTG               C^AATTG
MvnI         1932  CGCG                 CG^CG
NdeI         2663  CATATG               CA^TATG
NsiI         2666  ATGCAT               ATGCA^T
NspV          204  TTCGAA               TT^CGAA
PaeR7I        171  CTCGAG               C^TCGAG
PflFI        3988  GACAGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI         858  CCAACAAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI        2335  ACCGGT               A^CCGGT
PmaCI        3537  CACGTG               CAC^GTG
PmlI         3537  CACGTG               CAC^GTG
PshAI        1484  GACCAGGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI        3423  ATTAAT               AT^TAAT
Psp1406I     2468  AACGTT               AA^CGTT
SacI         3469  GAGCTC               GAGCT^C
ScaI         3570  AGTACT               AGT^ACT
SfuI          204  TTCGAA               TT^CGAA
SmaI         4058  CCCGGG               CCC^GGG
SpeI         3304  ACTAGT               A^CTAGT
SwaI         3367  ATTTAAAT             ATTT^AAAT
Tth111I      3988  GACAGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I        858  CCAACAAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI          171  CTCGAG               C^TCGAG
XmaCI        4058  CCCGGG               C^CCGGG
XmaI         4058  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   35 enzymes) #
###################################

AlwNI        2099  CAGCCACTG            CAG[ACGT][ACGT][ACGT]^CTG
             2370  CAGTAACTG           
AocI         3922  CCTCAGG              CC^T[ACGT]AGG
             4196  CCTCAGG             
Asp700I       115  GAATATTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2854  GAAGATCTTC          
AspHI        3469  GAGCTC               G[AT]GC[AT]^C
             4254  GTGCAC              
AvaI          171  CTCGAG               C^[CT]CG[GA]G
             4058  CCCGGG              
BalI          329  TGGCCA               TGG^CCA
             2485  TGGCCA              
BanII        1146  GAGCCC               G[GA]GC[CT]^C
             3469  GAGCTC              
BbsI           29  GAAGAC               GAAGAC(2/6)
             1813  GAAGAC              
BciVI        1262  GGATAC               GTATCC(6/5)
             2814  GGATAC              
BcnI         4058  CCCGG                CC^[GC]GG
             4059  CCGGG               
BglII        1786  AGATCT               A^GATCT
             2856  AGATCT              
BlpI         2768  GCTGAGC              GC^T[ACGT]AGC
             2940  GCTTAGC             
BpmI         3220  CTCCAG               CTGGAG(16/14)
             4014  CTGGAG              
Bpu1102I     2768  GCTGAGC              GC^T[ACGT]AGC
             2940  GCTTAGC             
BpuAI          29  GAAGAC               GAAGAC(2/6)
             1813  GAAGAC              
BsaWI        1968  ACCGGA               [AT]^CCGG[AT]
             2335  ACCGGT              
BsiHKAI      3469  GAGCTC               G[AT]GC[AT]^C
             4254  GTGCAC              
BsoBI         171  CTCGAG               C^[CT]CG[GA]G
             4058  CCCGGG              
BstXI         410  CCAGGTTCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             4161  CCACCATATTGG        
BstYI        1786  AGATCT               [GA]^GATC[CT]
             2856  AGATCT              
Bsu36I       3922  CCTCAGG              CC^T[ACGT]AGG
             4196  CCTCAGG             
CelII        2768  GCTGAGC              GC^T[ACGT]AGC
             2940  GCTTAGC             
DsaI          104  CCATGG               C^C[GA][CT]GG
             1760  CCATGG              
EaeI          329  TGGCCA               [CT]^GGCC[GA]
             2485  TGGCCA              
Eco81I       3922  CCTCAGG              CC^T[ACGT]AGG
             4196  CCTCAGG             
HgaI         1199  GACGC                GACGC(5/10)
             4300  GCGTC               
HpaI         1130  GTTAAC               GTT^AAC
             3300  GTTAAC              
MflI         1786  AGATCT               [GA]^GATC[CT]
             2856  AGATCT              
MluNI         329  TGGCCA               TGG^CCA
             2485  TGGCCA              
MscI          329  TGGCCA               TGG^CCA
             2485  TGGCCA              
NciI         4058  CCCGG                CC^[GC]GG
             4059  CCGGG               
NcoI          104  CCATGG               C^CATGG
             1760  CCATGG              
SapI         3466  GAAGAGC              GCTCTTC(1/4)
             4340  GAAGAGC             
XhoII        1786  AGATCT               [GA]^GATC[CT]
             2856  AGATCT              
XmnI          115  GAATATTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2854  GAAGATCTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   95 enzymes) #
#########################################

AciI        AcsI        AcyI        AfaI        AluI        
AlwI        ApoI        AvaII       BbvI        BfaI        
BmyI        BsaBI       BsaHI       BsaJI       BsgI        
BsiYI       BslI        BsmAI       BsmFI       BsmI        
Bsp1286I    Bsp1407I    BspHI       BspMI       BsrDI       
BsrGI       BsrI        BssKI       BstAPI      BstF5I      
BstNI       Cac8I       CfoI        Cfr13I      DdeI        
DpnII       DraI        EarI        EcoRI       EcoRII      
EcoT14I     Fnu4HI      FokI        HaeIII      HapII       
HhaI        Hin1I       HinP1I      HincII      HindII      
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeI        MaeII       MaeIII      MamI        MboI        
MboII       MnlI        MseI        MslI        MspA1I      
MspI        MvaI        MwoI        NdeII       NlaIII      
NlaIV       NspI        PleI        PstI        PvuII       
RcaI        RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SmlI        SphI        SspBI       
SspI        StyI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I