##################################
# NO CUT ENZYMES ( 62 enzymes) #
##################################
AatII Acc65I AclI AcsI AflII
ApoI AscI AseI AsnI Asp700I
Asp718I AvrII BfrI BglII BlnI
BsaBI BsiWI BsmBI BspDI BspLU11I
BssSI Bst1107I BstBI BstZ17I ClaI
CpoI DraI DrdI EcoRI EcoRV
EcoT22I FseI HindIII HpaI KpnI
MamI MfeI MunI NdeI NotI
NruI NsiI NspV PacI PmeI
PshAI PshBI Psp1406I PvuI RsrII
SalI SbfI ScaI SfiI SfuI
SnaBI SpeI Sse8387I SspI SwaI
XbaI XmnI
###################################
# ONE CUT ENZYMES ( 30 enzymes) #
###################################
AccI 4789 GTCTAC GT^[AC][GT]AC
AflIII 2223 ACGCGT A^C[GA][CT]GT
AgeI 2644 ACCGGT A^CCGGT
AhdI 5367 GACCAACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 4344 AGCGCT AGC^GCT
AspEI 5367 GACCAACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 3744 TGCGCA TGC^GCA
BbeI 542 GGCGCC GGCGC^C
BbrPI 1843 CACGTG CAC^GTG
BclI 5733 TGATCA T^GATCA
BsmI 5397 GAATGC GAATGC(1/-1)
BssHII 4128 GCGCGC G^CGCGC
Eam1105I 5367 GACCAACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 4344 AGCGCT AGC^GCT
FbaI 5733 TGATCA T^GATCA
FspI 3744 TGCGCA TGC^GCA
HincII 3412 GTCAAC GT[CT]^[GA]AC
HindII 3412 GTCAAC GT[CT]^[GA]AC
KasI 542 GGCGCC G^GCGCC
MluI 2223 ACGCGT A^CGCGT
NarI 542 GGCGCC GG^CGCC
PaeR7I 361 CTCGAG C^TCGAG
PinAI 2644 ACCGGT A^CCGGT
PmaCI 1843 CACGTG CAC^GTG
PmlI 1843 CACGTG CAC^GTG
SfoI 542 GGCGCC GGC^GCC
SgrAI 33 CGCCGGTG C[GA]^CCGG[CT]G
SphI 4827 GCATGC GCATG^C
Tsp509I 6045 AATT ^AATT
XhoI 361 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
Alw44I 3676 GTGCAC G^TGCAC
4417 GTGCAC
ApaLI 3676 GTGCAC G^TGCAC
4417 GTGCAC
BciVI 1517 GGATAC GTATCC(6/5)
3365 GTATCC
BspHI 4049 TCATGA T^CATGA
4379 TCATGA
BsrBI 837 CCGCTC CCGCTC(-3/-3)
891 CCGCTC
BsrDI 1248 CATTGC GCAATG(2/0)
3803 GCAATG
BstEII 4396 GGTGACC G^GT[ACGT]ACC
4864 GGTCACC
BstPI 4396 GGTGACC G^GT[ACGT]ACC
4864 GGTCACC
EcoO65I 4396 GGTGACC G^GT[ACGT]ACC
4864 GGTCACC
MseI 1883 TTAA T^TAA
2843 TTAA
NaeI 2307 GCCGGC GCC^GGC
2738 GCCGGC
NgoMIV 2307 GCCGGC G^CCGGC
2738 GCCGGC
NheI 4341 GCTAGC G^CTAGC
5316 GCTAGC
NspI 4538 ACATGC [GA]CATG^[CT]
4827 GCATGC
RcaI 4049 TCATGA T^CATGA
4379 TCATGA
SapI 4254 GCTCTTC GCTCTTC(1/4)
4542 GCTCTTC
Tru9I 1883 TTAA T^TAA
2843 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 149 enzymes) #
#########################################
AccII AccIII AciI AcyI AfaI
AluI AlwI AlwNI AocI ApaI
AspHI AspI AvaI AvaII BalI
BamHI BanI BanII BbsI BbvCI
BbvI BcgI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaAI BsaHI BsaI BsaJI BsaWI
BseAI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I Bsp1407I BspEI BspMI BsrFI
BsrGI BsrI BssKI BstAPI BstF5I
BstNI BstUI BstXI BstYI Bsu36I
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DraIII DsaI
EaeI EagI EarI EclXI Eco52I
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
KspI MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MroI
MscI MslI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV PflFI PflMI PleI
PpuMI PstI PvuII RsaI SacI
SacII Sau3AI Sau96I ScrFI SexAI
SfaNI SfcI SmaI SmlI SspBI
StuI StyI TaqI TfiI TseI
Tsp45I TspRI Tth111I TthHB8I Van91I
XcmI XhoII XmaCI XmaI