##################################
# NO CUT ENZYMES (   67 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AgeI        
Aor51HI     AscI        Asp718I     BamHI       BbeI        
BcgI        BsaBI       BseAI       BsiEI       BsiWI       
BspDI       BspEI       BssHII      Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstUI       BstZ17I     
ClaI        CpoI        EagI        EclXI       Eco47III    
Eco52I      EcoNI       EcoO65I     EcoRV       FseI        
KasI        KpnI        KspI        MamI        MluI        
MroI        MvnI        NarI        NheI        NotI        
NruI        NspV        PacI        PaeR7I      PinAI       
PmeI        PshAI       PvuI        RsrII       SacII       
SalI        SbfI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SnaBI       Sse8387I    
SwaI        XhoI        


###################################
# ONE CUT ENZYMES (   45 enzymes) #
###################################

AcyI          561  GGCGTC               G[GA]^CG[CT]C
AocI          229  CCTGAGG              CC^T[ACGT]AGG
AseI         4430  ATTAAT               AT^TAAT
AsnI         4430  ATTAAT               AT^TAAT
Asp700I      2877  GAAGCCTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII        3884  TGCGCA               TGC^GCA
AvrII        2947  CCTAGG               C^CTAGG
BalI         1727  TGGCCA               TGG^CCA
BbsI         2061  GTCTTC               GAAGAC(2/6)
BbvCI        5473  GCTGAGG              CCTCAGC(-5/-2)
BciVI        6022  GGATAC               GTATCC(6/5)
BclI         3274  TGATCA               T^GATCA
BlnI         2947  CCTAGG               C^CTAGG
BlpI          736  GCTAAGC              GC^T[ACGT]AGC
Bpu1102I      736  GCTAAGC              GC^T[ACGT]AGC
BpuAI        2061  GTCTTC               GAAGAC(2/6)
BsaHI         561  GGCGTC               G[GA]^CG[CT]C
BsaWI        5568  TCCGGT               [AT]^CCGG[AT]
BsmI         1383  GCATTC               GAATGC(1/-1)
Bsp1407I     2469  TGTACA               T^GTACA
BspLU11I     2908  ACATGT               A^CATGT
BspMI        3398  GCAGGT               ACCTGC(4/8)
BsrGI        2469  TGTACA               T^GTACA
BssSI        2579  CTCGTG               CACGAG(-5/-1)
Bsu36I        229  CCTGAGG              CC^T[ACGT]AGG
CelII         736  GCTAAGC              GC^T[ACGT]AGC
DraIII        487  CACTGGGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI         5679  GACTGAGCTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I        229  CCTGAGG              CC^T[ACGT]AGG
EcoRI        3789  GAATTC               G^AATTC
FbaI         3274  TGATCA               T^GATCA
FspI         3884  TGCGCA               TGC^GCA
Hin1I         561  GGCGTC               G[GA]^CG[CT]C
MluNI        1727  TGGCCA               TGG^CCA
MscI         1727  TGGCCA               TGG^CCA
NaeI          497  GCCGGC               GCC^GGC
NgoMIV        497  GCCGGC               G^CCGGC
PflMI        2789  CCAGTAAATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI        4430  ATTAAT               AT^TAAT
SmaI          504  CCCGGG               CCC^GGG
SspBI        2469  TGTACA               T^GTACA
Van91I       2789  CCAGTAAATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI         504  CCCGGG               C^CCGGG
XmaI          504  CCCGGG               C^CCGGG
XmnI         2877  GAAGCCTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   31 enzymes) #
###################################

AccI         1117  GTAGAC               GT^[AC][GT]AC
             2436  GTAGAC              
AclI         4536  AACGTT               AA^CGTT
             5409  AACGTT              
AflII        3365  CTTAAG               C^TTAAG
             5149  CTTAAG              
AflIII       2908  ACATGT               A^C[GA][CT]GT
             6099  ACACGT              
AhdI         1438  GACTCCGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1777  GACAGCAAGTC         
Alw44I       2912  GTGCAC               G^TGCAC
             3709  GTGCAC              
ApaI          941  GGGCCC               GGGCC^C
             1527  GGGCCC              
ApaLI        2912  GTGCAC               G^TGCAC
             3709  GTGCAC              
AspEI        1438  GACTCCGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1777  GACAGCAAGTC         
AspI          838  GACAAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2826  GACTGTGTC           
BfrI         3365  CTTAAG               C^TTAAG
             5149  CTTAAG              
BglII         670  AGATCT               A^GATCT
             4333  AGATCT              
BspHI        4675  TCATGA               T^CATGA
             5814  TCATGA              
BsrFI         497  GCCGGC               [GA]^CCGG[CT]
             3893  GCCGGT              
BstXI         181  CCATCGCATTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              683  CCAGCCCCTTGG        
Cfr10I        497  GCCGGC               [GA]^CCGG[CT]
             3893  GCCGGT              
Eam1105I     1438  GACTCCGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1777  GACAGCAAGTC         
EcoT22I      3773  ATGCAT               ATGCA^T
             3777  ATGCAT              
HaeII        1630  AGCGCC               [GA]GCGC^[CT]
             1841  GGCGCT              
HpaI         3055  GTTAAC               GTT^AAC
             5734  GTTAAC              
MfeI         2342  CAATTG               C^AATTG
             2465  CAATTG              
MunI         2342  CAATTG               C^AATTG
             2465  CAATTG              
NdeI         3287  CATATG               CA^TATG
             3770  CATATG              
NsiI         3773  ATGCAT               ATGCA^T
             3777  ATGCAT              
PflFI         838  GACAAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2826  GACTGTGTC           
Psp1406I     4536  AACGTT               AA^CGTT
             5409  AACGTT              
RcaI         4675  TCATGA               T^CATGA
             5814  TCATGA              
SapI         1560  GAAGAGC              GCTCTTC(1/4)
             5882  GAAGAGC             
SpeI         4440  ACTAGT               A^CTAGT
             6037  ACTAGT              
StuI         1520  AGGCCT               AGG^CCT
             4980  AGGCCT              
Tth111I       838  GACAAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2826  GACTGTGTC           


#########################################
# 3 OR MORE CUT ENZYMES (  115 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
AlwNI       ApoI        AspHI       AvaI        AvaII       
BanI        BanII       BbrPI       BbvI        BcnI        
BfaI        BglI        BmyI        BpmI        BsaAI       
BsaI        BsaJI       BseRI       BsgI        BsiHKAI     
BsiYI       BslI        BsmAI       BsmBI       BsmFI       
BsoBI       Bsp1286I    BsrBI       BsrDI       BsrI        
BssKI       BstF5I      BstNI       BstYI       Cac8I       
CfoI        Cfr13I      DdeI        DpnII       DraI        
DraII       DsaI        EaeI        EarI        EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeIII      HapII       HgaI        HhaI        HinP1I      
HincII      HindII      HindIII     HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MnlI        
MseI        MslI        MspA1I      MspI        MvaI        
MwoI        NciI        NcoI        NdeII       NlaIII      
NlaIV       NspI        PleI        PmaCI       PmlI        
PpuMI       PstI        PvuII       RsaI        SacI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmlI        SphI        SspI        StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     XbaI        XcmI        XhoII