##################################
# NO CUT ENZYMES ( 95 enzymes) #
##################################
AatII AccIII AclI AcsI AcyI
AgeI AhdI Alw44I ApaLI ApoI
AscI AseI AsnI AspEI AspI
AvrII BbeI BbsI BciVI BclI
BglII BlnI BpuAI BsaBI BsaHI
BsaWI BseAI BsiEI BsiWI BsmBI
BspDI BspEI BsrBI BssHII BssSI
Bst1107I BstBI BstZ17I ClaI CpoI
DraI DraIII EagI Eam1105I EclXI
Eco52I EcoRI EcoRV FbaI FseI
Hin1I HincII HindII HindIII HpaI
KasI KspI MamI MfeI MluI
MroI MunI NarI NdeI NheI
NotI NruI NspV PacI PaeR7I
PflFI PinAI PmeI PshAI PshBI
Psp1406I PstI PvuI RsrII SacII
SalI SbfI ScaI SexAI SfoI
SfuI SgrAI SnaBI SphI Sse8387I
SspI SwaI Tsp509I Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 30 enzymes) #
###################################
Acc65I 251 GGTACC G^GTACC
AccI 688 GTCTAC GT^[AC][GT]AC
AflII 2844 CTTAAG C^TTAAG
AflIII 2812 ACATGT A^C[GA][CT]GT
Aor51HI 992 AGCGCT AGC^GCT
Asp718I 251 GGTACC G^GTACC
AviII 275 TGCGCA TGC^GCA
BamHI 2177 GGATCC G^GATCC
BbrPI 966 CACGTG CAC^GTG
BbvCI 534 CCTCAGC CCTCAGC(-5/-2)
BfrI 2844 CTTAAG C^TTAAG
BsaAI 966 CACGTG [CT]AC^GT[GA]
Bsp1407I 230 TGTACA T^GTACA
BspLU11I 2812 ACATGT A^CATGT
BsrDI 782 GCAATG GCAATG(2/0)
BsrGI 230 TGTACA T^GTACA
Eco47III 992 AGCGCT AGC^GCT
EcoT22I 2867 ATGCAT ATGCA^T
FspI 275 TGCGCA TGC^GCA
HgaI 41 GCGTC GACGC(5/10)
KpnI 251 GGTACC GGTAC^C
NcoI 1554 CCATGG C^CATGG
NsiI 2867 ATGCAT ATGCA^T
PmaCI 966 CACGTG CAC^GTG
PmlI 966 CACGTG CAC^GTG
SfiI 1942 GGCCAAAAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SpeI 2556 ACTAGT A^CTAGT
SspBI 230 TGTACA T^GTACA
StuI 1499 AGGCCT AGG^CCT
XbaI 2829 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AocI 574 CCTCAGG CC^T[ACGT]AGG
2374 CCTCAGG
Asp700I 166 GAATACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2638 GAATGCCTTC
AspHI 801 GAGCTC G[AT]GC[AT]^C
1105 GAGCTC
BalI 666 TGGCCA TGG^CCA
1754 TGGCCA
BlpI 798 GCTGAGC GC^T[ACGT]AGC
1571 GCTCAGC
Bpu1102I 798 GCTGAGC GC^T[ACGT]AGC
1571 GCTCAGC
BsaI 790 GAGACC GGTCTC(1/5)
1391 GGTCTC
BsiHKAI 801 GAGCTC G[AT]GC[AT]^C
1105 GAGCTC
BsmI 1744 GAATGC GAATGC(1/-1)
2638 GAATGC
BspHI 855 TCATGA T^CATGA
2491 TCATGA
BsrFI 539 GCCGGC [GA]^CCGG[CT]
1112 GCCGGC
BstEII 471 GGTCACC G^GT[ACGT]ACC
2454 GGTGACC
BstPI 471 GGTCACC G^GT[ACGT]ACC
2454 GGTGACC
BstXI 1617 CCACTCAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2189 CCATCCGGCTGG
Bsu36I 574 CCTCAGG CC^T[ACGT]AGG
2374 CCTCAGG
CelII 798 GCTGAGC GC^T[ACGT]AGC
1571 GCTCAGC
Cfr10I 539 GCCGGC [GA]^CCGG[CT]
1112 GCCGGC
DrdI 1636 GACTCTGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1841 GACACTGGAGTC
DsaI 1554 CCATGG C^C[GA][CT]GG
1978 CCACGG
Eco81I 574 CCTCAGG CC^T[ACGT]AGG
2374 CCTCAGG
EcoO65I 471 GGTCACC G^GT[ACGT]ACC
2454 GGTGACC
MaeII 967 ACGT A^CGT
2216 ACGT
MluNI 666 TGGCCA TGG^CCA
1754 TGGCCA
MscI 666 TGGCCA TGG^CCA
1754 TGGCCA
MseI 2845 TTAA T^TAA
2946 TTAA
NaeI 539 GCCGGC GCC^GGC
1112 GCCGGC
NgoMIV 539 GCCGGC G^CCGGC
1112 GCCGGC
PflMI 1020 CCACTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1092 CCAGCTCCTGG
RcaI 855 TCATGA T^CATGA
2491 TCATGA
SacI 801 GAGCTC GAGCT^C
1105 GAGCTC
SapI 1102 GAAGAGC GCTCTTC(1/4)
747 GCTCTTC
Tru9I 2845 TTAA T^TAA
2946 TTAA
Van91I 1020 CCACTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1092 CCAGCTCCTGG
XcmI 197 CCATGAAAGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1168 CCACTCCCCGCATGG
XmnI 166 GAATACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2638 GAATGCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 98 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI ApaI AvaI AvaII BanI
BanII BbvI BcgI BcnI BfaI
BglI BmyI BpmI BsaJI BseRI
BsgI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII EaeI EarI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MflI
MnlI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PpuMI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI SmlI StyI TaqI
TfiI TseI Tsp45I TspRI TthHB8I
XhoII XmaCI XmaI