##################################
# NO CUT ENZYMES (  108 enzymes) #
##################################

AatII       Acc65I      AccII       AclI        AcyI        
AgeI        AhdI        Alw44I      Aor51HI     ApaLI       
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AvrII       BamHI       BbeI        BbrPI       
BclI        BglI        BglII       BlnI        BlpI        
Bpu1102I    BsaAI       BsaBI       BsaHI       BsiEI       
BsiWI       BsmBI       BspDI       BspHI       BsrBI       
BsrFI       BssHII      BssSI       BstBI       BstEII      
BstPI       BstUI       CelII       Cfr10I      ClaI        
CpoI        DrdI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      EcoO65I     EcoRI       FauI        
FbaI        FseI        HaeII       HgaI        Hin1I       
HpaI        KasI        KpnI        KspI        MamI        
MluI        MvnI        NaeI        NarI        NdeI        
NgoMIV      NotI        NruI        NspV        PacI        
PaeR7I      PflMI       PinAI       PmaCI       PmeI        
PmlI        PshBI       Psp1406I    PvuI        PvuII       
RcaI        RsrII       SacI        SacII       SalI        
SapI        SbfI        ScaI        SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
Sse8387I    SspI        SwaI        Van91I      XhoI        
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

AccIII          9  TCCGGA               T^CCGGA
AflIII        474  ACATGT               A^C[GA][CT]GT
ApaI         2657  GGGCCC               GGGCC^C
AspHI        2862  GAGCAC               G[AT]GC[AT]^C
AspI          848  GACTATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI          128  CTCGGG               C^[CT]CG[GA]G
AviII         488  TGCGCA               TGC^GCA
BalI         3871  TGGCCA               TGG^CCA
BanI          284  GGCACC               G^G[CT][GA]CC
BcgI         1542  GCAGGATACTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaI         4042  GGTCTC               GGTCTC(1/5)
BseAI           9  TCCGGA               T^CCGGA
BsgI         3894  GTGCAG               GTGCAG(16/14)
BsiHKAI      2862  GAGCAC               G[AT]GC[AT]^C
BsoBI         128  CTCGGG               C^[CT]CG[GA]G
Bsp1407I      119  TGTACA               T^GTACA
BspEI           9  TCCGGA               T^CCGGA
BspLU11I      474  ACATGT               A^CATGT
BsrGI         119  TGTACA               T^GTACA
BstAPI       1504  GCAGCCGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CfoI          489  GCGC                 GCG^C
DraI          816  TTTAAA               TTT^AAA
EcoNI        1030  CCTGGTAGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV         901  GATATC               GAT^ATC
FspI          488  TGCGCA               TGC^GCA
HhaI          489  GCGC                 GCG^C
HinP1I        489  GCGC                 G^CGC
HincII       2414  GTTGAC               GT[CT]^[GA]AC
HindII       2414  GTTGAC               GT[CT]^[GA]AC
MaeII         134  ACGT                 A^CGT
MfeI         2143  CAATTG               C^AATTG
MluNI        3871  TGGCCA               TGG^CCA
MroI            9  TCCGGA               T^CCGGA
MscI         3871  TGGCCA               TGG^CCA
MunI         2143  CAATTG               C^AATTG
NheI         1192  GCTAGC               G^CTAGC
PflFI         848  GACTATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
SexAI        1029  ACCTGGT              A^CC[AT]GGT
SphI         2295  GCATGC               GCATG^C
SspBI         119  TGTACA               T^GTACA
TaqI         3990  TCGA                 T^CGA
Tth111I       848  GACTATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I      3990  TCGA                 T^CGA
XcmI         3365  CCAGAGGTAGCTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   17 enzymes) #
###################################

BbvCI        3851  CCTCAGC              CCTCAGC(-5/-2)
             3623  GCTGAGG             
BcnI           68  CCCGG                CC^[GC]GG
             2812  CCCGG               
BpmI         3174  CTGGAG               CTGGAG(16/14)
             3845  CTGGAG              
BsaWI           9  TCCGGA               [AT]^CCGG[AT]
             2767  ACCGGA              
BsmI         1802  GAATGC               GAATGC(1/-1)
             2771  GAATGC              
BsrDI        1387  CATTGC               GCAATG(2/0)
             4096  CATTGC              
Bst1107I      638  GTATAC               GTA^TAC
             2526  GTATAC              
BstXI        3309  CCAACAGGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3701  CCATTACTCTGG        
BstZ17I       638  GTATAC               GTA^TAC
             2526  GTATAC              
DraIII        710  CACTGTGTG            CAC[ACGT][ACGT][ACGT]^GTG
             4102  CACAGTGTG           
EcoT22I      2258  ATGCAT               ATGCA^T
             2293  ATGCAT              
HindIII       541  AAGCTT               A^AGCTT
             2871  AAGCTT              
MspA1I       1499  CCGCTG               C[AC]G^C[GT]G
             1508  CCGCTG              
NciI           68  CCCGG                CC^[GC]GG
             2812  CCCGG               
NcoI         1638  CCATGG               C^CATGG
             2583  CCATGG              
NsiI         2258  ATGCAT               ATGCA^T
             2293  ATGCAT              
PshAI        2900  GACCAGAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
             3284  GACAATAGTC          


#########################################
# 3 OR MORE CUT ENZYMES (   89 enzymes) #
#########################################

AccI        AciI        AcsI        AfaI        AflII       
AluI        AlwI        AlwNI       AocI        ApoI        
AvaII       BanII       BbsI        BbvI        BciVI       
BfaI        BfrI        BmyI        BpuAI       BsaJI       
BseRI       BsiYI       BslI        BsmAI       BsmFI       
Bsp1286I    BspMI       BsrI        BssKI       BstF5I      
BstNI       BstYI       Bsu36I      Cac8I       Cfr13I      
DdeI        DpnII       DraII       DsaI        EaeI        
EarI        Eco81I      EcoO109I    EcoRII      EcoT14I     
Fnu4HI      FokI        HaeIII      HapII       HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeI        
MaeIII      MboI        MboII       MflI        MnlI        
MseI        MslI        MspI        MvaI        MwoI        
NdeII       NlaIII      NlaIV       NspI        PleI        
PpuMI       PstI        RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SmlI        StuI        
StyI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       XbaI        XhoII