##################################
# NO CUT ENZYMES ( 64 enzymes) #
##################################
Acc65I AclI AgeI AhdI AscI
Asp718I AspEI AviII BbeI BbrPI
BciVI BclI BsaAI BsaBI BsiWI
Bsp1407I BsrGI Bst1107I BstZ17I CpoI
EagI Eam1105I EclXI Eco52I FbaI
FseI FspI HindIII HpaI KasI
KpnI KspI MamI MluI NaeI
NarI NgoMIV NotI NruI PacI
PaeR7I PinAI PmaCI PmeI PmlI
Psp1406I RsrII SacII SalI SbfI
SfiI SfoI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SwaI
XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AatII 5450 GACGTC GACGT^C
AccI 1336 GTCTAC GT^[AC][GT]AC
AccII 1501 CGCG CG^CG
AccIII 451 TCCGGA T^CCGGA
AflII 3043 CTTAAG C^TTAAG
AseI 4689 ATTAAT AT^TAAT
AsnI 4689 ATTAAT AT^TAAT
BcgI 5571 GCAGGAGAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 325 CCCGG CC^[GC]GG
BfrI 3043 CTTAAG C^TTAAG
BsaWI 451 TCCGGA [AT]^CCGG[AT]
BseAI 451 TCCGGA T^CCGGA
BsiEI 825 CGATCG CG[GA][CT]^CG
BspDI 405 ATCGAT AT^CGAT
BspEI 451 TCCGGA T^CCGGA
BssHII 1500 GCGCGC G^CGCGC
BstBI 2236 TTCGAA TT^CGAA
BstUI 1501 CGCG CG^CG
ClaI 405 ATCGAT AT^CGAT
EcoRI 1841 GAATTC G^AATTC
EcoRV 2854 GATATC GAT^ATC
HgaI 5526 GCGTC GACGC(5/10)
MfeI 2675 CAATTG C^AATTG
MroI 451 TCCGGA T^CCGGA
MunI 2675 CAATTG C^AATTG
MvnI 1501 CGCG CG^CG
NciI 325 CCCGG CC^[GC]GG
NheI 4566 GCTAGC G^CTAGC
NspV 2236 TTCGAA TT^CGAA
PshAI 3412 GACATTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 4689 ATTAAT AT^TAAT
PvuI 825 CGATCG CGAT^CG
ScaI 292 AGTACT AGT^ACT
SfuI 2236 TTCGAA TT^CGAA
SspI 4113 AATATT AAT^ATT
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AcyI 5450 GACGTC G[GA]^CG[CT]C
5525 GGCGTC
Alw44I 1532 GTGCAC G^TGCAC
3676 GTGCAC
Aor51HI 645 AGCGCT AGC^GCT
1892 AGCGCT
ApaLI 1532 GTGCAC G^TGCAC
3676 GTGCAC
Asp700I 176 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1684 GAACACTTTC
AvaI 1083 CCCGAG C^[CT]CG[GA]G
5557 CTCGGG
AvrII 2594 CCTAGG C^CTAGG
4595 CCTAGG
BamHI 19 GGATCC G^GATCC
2883 GGATCC
BglI 432 GCCAGCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3237 GCCCGACTGGC
BglII 2448 AGATCT A^GATCT
3182 AGATCT
BlnI 2594 CCTAGG C^CTAGG
4595 CCTAGG
BsaHI 5450 GACGTC G[GA]^CG[CT]C
5525 GGCGTC
BsmBI 5661 CGTCTC CGTCTC(1/5)
2104 GAGACG
BsoBI 1083 CCCGAG C^[CT]CG[GA]G
5557 CTCGGG
BspHI 97 TCATGA T^CATGA
2767 TCATGA
BspLU11I 112 ACATGT A^CATGT
568 ACATGT
BsrBI 3904 CCGCTC CCGCTC(-3/-3)
4729 CCGCTC
BsrFI 229 ACCGGC [GA]^CCGG[CT]
1168 GCCGGT
BstEII 74 GGTGACC G^GT[ACGT]ACC
5045 GGTTACC
BstPI 74 GGTGACC G^GT[ACGT]ACC
5045 GGTTACC
Cfr10I 229 ACCGGC [GA]^CCGG[CT]
1168 GCCGGT
DsaI 2832 CCATGG C^C[GA][CT]GG
5128 CCATGG
Eco47III 645 AGCGCT AGC^GCT
1892 AGCGCT
EcoO65I 74 GGTGACC G^GT[ACGT]ACC
5045 GGTTACC
EcoT22I 4552 ATGCAT ATGCA^T
4978 ATGCAT
Hin1I 5450 GACGTC G[GA]^CG[CT]C
5525 GGCGTC
NcoI 2832 CCATGG C^CATGG
5128 CCATGG
NdeI 2934 CATATG CA^TATG
3763 CATATG
NsiI 4552 ATGCAT ATGCA^T
4978 ATGCAT
RcaI 97 TCATGA T^CATGA
2767 TCATGA
SacI 63 GAGCTC GAGCT^C
84 GAGCTC
SapI 4006 GAAGAGC GCTCTTC(1/4)
3906 GCTCTTC
StuI 1861 AGGCCT AGG^CCT
4897 AGGCCT
XmnI 176 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1684 GAACACTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 125 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI AocI ApaI ApoI
AspHI AspI AvaII BalI BanI
BanII BbsI BbvCI BbvI BfaI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
Bsp1286I BspMI BsrDI BsrI BssKI
BssSI BstAPI BstF5I BstNI BstXI
BstYI Bsu36I Cac8I CelII CfoI
Cfr13I DdeI DpnII DraI DraII
DraIII DrdI EaeI EarI Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MwoI NdeII
NlaIII NlaIV NspI PflFI PflMI
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SexAI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Tth111I TthHB8I Van91I XcmI XhoII