##################################
# NO CUT ENZYMES ( 68 enzymes) #
##################################
Acc65I AccIII AclI AgeI Aor51HI
AscI Asp718I AviII BbeI BbvCI
BclI BglII BsaBI BsaWI BseAI
BsiEI BsiWI BspDI BspEI BsrBI
BsrFI BssHII BstBI BstEII BstPI
Cfr10I ClaI CpoI EagI EclXI
Eco47III Eco52I EcoO65I EcoRV FbaI
FseI FspI HgaI KasI KpnI
MamI MluI MroI NaeI NarI
NgoMIV NotI NruI NspV PaeR7I
PinAI PshAI Psp1406I PvuI RsrII
SalI SbfI SexAI SfiI SfoI
SfuI SgrAI SnaBI SphI Sse8387I
SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AflII 97 CTTAAG C^TTAAG
Alw44I 637 GTGCAC G^TGCAC
ApaI 6032 GGGCCC GGGCC^C
ApaLI 637 GTGCAC G^TGCAC
Asp700I 2079 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 3453 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 4590 TGGCCA TGG^CCA
BamHI 174 GGATCC G^GATCC
BanI 1827 GGTGCC G^G[CT][GA]CC
BbrPI 3886 CACGTG CAC^GTG
BfrI 97 CTTAAG C^TTAAG
BsaAI 3886 CACGTG [CT]AC^GT[GA]
BsmBI 4157 GAGACG CGTCTC(1/5)
BsmI 3204 GAATGC GAATGC(1/-1)
Bst1107I 908 GTATAC GTA^TAC
BstZ17I 908 GTATAC GTA^TAC
CfoI 17 GCGC GCG^C
DraIII 196 CACCGAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 560 GACTTGTCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
HaeII 16 GGCGCT [GA]GCGC^[CT]
HhaI 17 GCGC GCG^C
HinP1I 17 GCGC G^CGC
KspI 833 CCGCGG CCGC^GG
MfeI 2774 CAATTG C^AATTG
MluNI 4590 TGGCCA TGG^CCA
MscI 4590 TGGCCA TGG^CCA
MunI 2774 CAATTG C^AATTG
NdeI 5094 CATATG CA^TATG
NheI 2670 GCTAGC G^CTAGC
PacI 3757 TTAATTAA TTAAT^TAA
PflFI 3453 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 4147 CCAAGATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 3886 CACGTG CAC^GTG
PmeI 3001 GTTTAAAC GTTT^AAAC
PmlI 3886 CACGTG CAC^GTG
SacI 2096 GAGCTC GAGCT^C
SacII 833 CCGCGG CCGC^GG
SapI 1281 GAAGAGC GCTCTTC(1/4)
SmaI 1034 CCCGGG CCC^GGG
TaqI 789 TCGA T^CGA
Tth111I 3453 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 789 TCGA T^CGA
Van91I 4147 CCAAGATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 221 CCAGGGGTTAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 1034 CCCGGG C^CCGGG
XmaI 1034 CCCGGG C^CCGGG
XmnI 2079 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AatII 4159 GACGTC GACGT^C
4219 GACGTC
AccII 834 CGCG CG^CG
4215 CGCG
AcyI 4159 GACGTC G[GA]^CG[CT]C
4219 GACGTC
AhdI 442 GACTGTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4180 GACTGAGTGTC
AseI 2263 ATTAAT AT^TAAT
6136 ATTAAT
AsnI 2263 ATTAAT AT^TAAT
6136 ATTAAT
AspEI 442 GACTGTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4180 GACTGAGTGTC
BcgI 4553 CGAGTTTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1047 GCAATGAACTCG
BglI 1885 GCCATCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3075 GCCAATCTGGC
BlpI 1018 GCTCAGC GC^T[ACGT]AGC
5962 GCTCAGC
Bpu1102I 1018 GCTCAGC GC^T[ACGT]AGC
5962 GCTCAGC
BsaHI 4159 GACGTC G[GA]^CG[CT]C
4219 GACGTC
Bsp1407I 4925 TGTACA T^GTACA
5128 TGTACA
BspHI 461 TCATGA T^CATGA
1315 TCATGA
BsrGI 4925 TGTACA T^GTACA
5128 TGTACA
BssSI 394 CACGAG CACGAG(-5/-1)
2451 CTCGTG
BstUI 834 CGCG CG^CG
4215 CGCG
BstXI 3701 CCAAAGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4164 CCACATAATTGG
CelII 1018 GCTCAGC GC^T[ACGT]AGC
5962 GCTCAGC
EaeI 3589 CGGCCA [CT]^GGCC[GA]
4590 TGGCCA
Eam1105I 442 GACTGTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4180 GACTGAGTGTC
EcoRI 2473 GAATTC G^AATTC
5429 GAATTC
FauI 626 GCGGG CCCGC(4/6)
644 GCGGG
Hin1I 4159 GACGTC G[GA]^CG[CT]C
4219 GACGTC
HindIII 547 AAGCTT A^AGCTT
2349 AAGCTT
HpaI 2301 GTTAAC GTT^AAC
3381 GTTAAC
MvnI 834 CGCG CG^CG
4215 CGCG
NcoI 412 CCATGG C^CATGG
3779 CCATGG
PpuMI 3416 GGGTCCC [GA]G^G[AT]CC[CT]
4063 GGGTCCC
PshBI 2263 ATTAAT AT^TAAT
6136 ATTAAT
RcaI 461 TCATGA T^CATGA
1315 TCATGA
ScaI 532 AGTACT AGT^ACT
1127 AGTACT
SpeI 1551 ACTAGT A^CTAGT
4920 ACTAGT
SspBI 4925 TGTACA T^GTACA
5128 TGTACA
SspI 1536 AATATT AAT^ATT
4963 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 108 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI AlwNI AocI ApoI
AspHI AvaI AvaII AvrII BanII
BbsI BbvI BciVI BcnI BfaI
BlnI BmyI BpmI BpuAI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspLU11I BspMI BsrDI BsrI BssKI
BstAPI BstF5I BstNI BstYI Bsu36I
Cac8I Cfr13I DdeI DpnII DraI
DraII DsaI EarI Eco81I EcoNI
EcoO109I EcoRII EcoT14I EcoT22I Fnu4HI
FokI HaeIII HapII HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NsiI NspI PleI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StuI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI XhoII