################################## # NO CUT ENZYMES ( 48 enzymes) # ################################## AgeI AhdI Alw44I ApaLI AscI AspEI AviII BciVI BclI BsiEI BsiWI BspDI BssHII Bst1107I BstBI BstZ17I ClaI CpoI DrdI EagI Eam1105I EclXI Eco52I EcoRV FbaI FseI FspI HindIII HpaI KspI MluI NdeI NheI NotI NruI NspV PacI PinAI PmeI PshAI PvuI RsrII SacII SalI SfiI SfuI SgrAI SwaI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AatII 319 GACGTC GACGT^C AccIII 101 TCCGGA T^CCGGA AflII 4787 CTTAAG C^TTAAG Aor51HI 4552 AGCGCT AGC^GCT AseI 1066 ATTAAT AT^TAAT AsnI 1066 ATTAAT AT^TAAT AspHI 734 GAGCTC G[AT]GC[AT]^C AspI 3129 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC AvrII 3675 CCTAGG C^CTAGG BbeI 3603 GGCGCC GGCGC^C BfrI 4787 CTTAAG C^TTAAG BglII 5612 AGATCT A^GATCT BlnI 3675 CCTAGG C^CTAGG BlpI 5750 GCTCAGC GC^T[ACGT]AGC Bpu1102I 5750 GCTCAGC GC^T[ACGT]AGC BsaBI 5404 GATTCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 101 TCCGGA [AT]^CCGG[AT] BseAI 101 TCCGGA T^CCGGA BsiHKAI 734 GAGCTC G[AT]GC[AT]^C BsmBI 4224 GAGACG CGTCTC(1/5) BspEI 101 TCCGGA T^CCGGA BspHI 1270 TCATGA T^CATGA BstXI 2653 CCACCACCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 5750 GCTCAGC GC^T[ACGT]AGC DraIII 2137 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG Eco47III 4552 AGCGCT AGC^GCT FauI 5300 CCCGC CCCGC(4/6) KasI 3603 GGCGCC G^GCGCC MamI 5404 GATTCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 4097 CAATTG C^AATTG MroI 101 TCCGGA T^CCGGA MunI 4097 CAATTG C^AATTG NaeI 227 GCCGGC GCC^GGC NarI 3603 GGCGCC GG^CGCC NgoMIV 227 GCCGGC G^CCGGC PaeR7I 2795 CTCGAG C^TCGAG PflFI 3129 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC PshBI 1066 ATTAAT AT^TAAT RcaI 1270 TCATGA T^CATGA SacI 734 GAGCTC GAGCT^C SbfI 3294 CCTGCAGG CCTGCA^GG ScaI 2900 AGTACT AGT^ACT SexAI 3130 ACCAGGT A^CC[AT]GGT SfoI 3603 GGCGCC GGC^GCC SpeI 5121 ACTAGT A^CTAGT SphI 1691 GCATGC GCATG^C Sse8387I 3294 CCTGCAGG CCTGCA^GG SspI 4711 AATATT AAT^ATT Tth111I 3129 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoI 2795 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### Acc65I 2565 GGTACC G^GTACC 6336 GGTACC AclI 3846 AACGTT AA^CGTT 6134 AACGTT ApaI 621 GGGCCC GGGCC^C 3783 GGGCCC Asp718I 2565 GGTACC G^GTACC 6336 GGTACC BbrPI 5393 CACGTG CAC^GTG 5883 CACGTG BcgI 1460 CGATTTTTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 1596 CGAGGAAAATGC BsrBI 5301 CCGCTC CCGCTC(-3/-3) 2482 GAGCGG BstAPI 1572 GCATTCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 5741 GCAGTGTCTGC BstEII 1895 GGTCACC G^GT[ACGT]ACC 2245 GGTCACC BstPI 1895 GGTCACC G^GT[ACGT]ACC 2245 GGTCACC DraI 673 TTTAAA TTT^AAA 4082 TTTAAA EcoO65I 1895 GGTCACC G^GT[ACGT]ACC 2245 GGTCACC EcoT22I 4436 ATGCAT ATGCA^T 6500 ATGCAT KpnI 2565 GGTACC GGTAC^C 6336 GGTACC NsiI 4436 ATGCAT ATGCA^T 6500 ATGCAT PflMI 1993 CCATTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2814 CCACCCTTTGG PmaCI 5393 CACGTG CAC^GTG 5883 CACGTG PmlI 5393 CACGTG CAC^GTG 5883 CACGTG Psp1406I 3846 AACGTT AA^CGTT 6134 AACGTT SapI 731 GAAGAGC GCTCTTC(1/4) 2801 GCTCTTC SnaBI 4851 TACGTA TAC^GTA 6259 TACGTA Van91I 1993 CCATTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2814 CCACCCTTTGG XbaI 1758 TCTAGA T^CTAGA 2016 TCTAGA XcmI 4513 CCAGTGTCCTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 5655 CCAGTTTGAGAATGG ######################################### # 3 OR MORE CUT ENZYMES ( 136 enzymes) # ######################################### AccI AccII AciI AcsI AcyI AfaI AflIII AluI AlwI AlwNI AocI ApoI Asp700I AvaI AvaII BalI BamHI BanI BanII BbsI BbvCI BbvI BcnI BfaI BglI BmyI BpmI BpuAI BsaAI BsaHI BsaI BsaJI BseRI BsgI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspLU11I BspMI BsrDI BsrFI BsrGI BsrI BssKI BssSI BstF5I BstNI BstUI BstYI Bsu36I Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoRI EcoRII EcoT14I Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI SspBI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII XmaCI XmaI XmnI