##################################
# NO CUT ENZYMES ( 47 enzymes) #
##################################
AatII AclI AflII AhdI AocI
AscI AseI AsnI AspEI AvrII
BamHI BciVI BfrI BlnI BsaBI
BsmI BspDI Bst1107I BstZ17I Bsu36I
ClaI DraI Eam1105I Eco81I EcoRV
HpaI MamI MfeI MunI NdeI
NotI NruI PacI PmeI PshAI
PshBI Psp1406I PvuI SalI SexAI
SfiI SgrAI SnaBI SpeI SspI
SwaI XbaI
###################################
# ONE CUT ENZYMES ( 22 enzymes) #
###################################
Acc65I 293 GGTACC G^GTACC
AccI 3265 GTCTAC GT^[AC][GT]AC
Asp700I 4887 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 293 GGTACC G^GTACC
AviII 4763 TGCGCA TGC^GCA
BglII 2081 AGATCT A^GATCT
BspLU11I 5270 ACATGT A^CATGT
BssHII 39 GCGCGC G^CGCGC
BstAPI 3894 GCAGTATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
EcoT22I 2121 ATGCAT ATGCA^T
FspI 4763 TGCGCA TGC^GCA
HindIII 5037 AAGCTT A^AGCTT
KpnI 293 GGTACC GGTAC^C
MluI 6567 ACGCGT A^CGCGT
NheI 3727 GCTAGC G^CTAGC
NsiI 2121 ATGCAT ATGCA^T
PaeR7I 6199 CTCGAG C^TCGAG
SbfI 4614 CCTGCAGG CCTGCA^GG
ScaI 4373 AGTACT AGT^ACT
Sse8387I 4614 CCTGCAGG CCTGCA^GG
XhoI 6199 CTCGAG C^TCGAG
XmnI 4887 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AccIII 452 TCCGGA T^CCGGA
5906 TCCGGA
AgeI 1565 ACCGGT A^CCGGT
5342 ACCGGT
Alw44I 1569 GTGCAC G^TGCAC
4203 GTGCAC
Aor51HI 4427 AGCGCT AGC^GCT
6115 AGCGCT
ApaLI 1569 GTGCAC G^TGCAC
4203 GTGCAC
BbeI 15 GGCGCC GGCGC^C
1414 GGCGCC
BbvCI 4298 CCTCAGC CCTCAGC(-5/-2)
4180 GCTGAGG
BcgI 3354 CGAGATCAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1694 GCAGGGTCATCG
BclI 2024 TGATCA T^GATCA
2486 TGATCA
BglI 6121 GCCCCTCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
6333 GCCAGCTCGGC
BsaI 736 GAGACC GGTCTC(1/5)
6227 GGTCTC
BseAI 452 TCCGGA T^CCGGA
5906 TCCGGA
BsiWI 3645 CGTACG C^GTACG
4950 CGTACG
BspEI 452 TCCGGA T^CCGGA
5906 TCCGGA
BspHI 2066 TCATGA T^CATGA
6413 TCATGA
BssSI 6149 CACGAG CACGAG(-5/-1)
6574 CACGAG
BstBI 1921 TTCGAA TT^CGAA
5849 TTCGAA
CpoI 970 CGGACCG CG^G[AT]CCG
5753 CGGACCG
Eco47III 4427 AGCGCT AGC^GCT
6115 AGCGCT
EcoRI 1924 GAATTC G^AATTC
2062 GAATTC
FbaI 2024 TGATCA T^GATCA
2486 TGATCA
KasI 15 GGCGCC G^GCGCC
1414 GGCGCC
MroI 452 TCCGGA T^CCGGA
5906 TCCGGA
MseI 4610 TTAA T^TAA
6776 TTAA
NarI 15 GGCGCC GG^CGCC
1414 GGCGCC
NcoI 3086 CCATGG C^CATGG
4121 CCATGG
NspV 1921 TTCGAA TT^CGAA
5849 TTCGAA
PinAI 1565 ACCGGT A^CCGGT
5342 ACCGGT
RcaI 2066 TCATGA T^CATGA
6413 TCATGA
RsrII 970 CGGACCG CG^G[AT]CCG
5753 CGGACCG
SacI 2070 GAGCTC GAGCT^C
3043 GAGCTC
SfoI 15 GGCGCC GGC^GCC
1414 GGCGCC
SfuI 1921 TTCGAA TT^CGAA
5849 TTCGAA
Tru9I 4610 TTAA T^TAA
6776 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 155 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI ApaI
ApoI AspHI AspI AvaI AvaII
BalI BanI BanII BbrPI BbsI
BbvI BcnI BfaI BlpI BmyI
BpmI Bpu1102I BpuAI BsaAI BsaHI
BsaJI BsaWI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsoBI Bsp1286I Bsp1407I BspMI
BsrBI BsrDI BsrFI BsrGI BsrI
BssKI BstEII BstF5I BstNI BstPI
BstUI BstXI BstYI Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DraIII DrdI DsaI EaeI
EagI EarI EclXI Eco52I EcoNI
EcoO109I EcoO65I EcoRII EcoT14I FauI
Fnu4HI FokI FseI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I KspI MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NciI
NdeII NgoMIV NlaIII NlaIV NspI
PflFI PflMI PleI PmaCI PmlI
PpuMI PstI PvuII RsaI SacII
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI SmlI SphI SspBI
StuI StyI TaqI TfiI TseI
Tsp45I Tsp509I TspRI Tth111I TthHB8I
Van91I XcmI XhoII XmaCI XmaI