##################################
# NO CUT ENZYMES ( 75 enzymes) #
##################################
AatII Acc65I AccIII AflII AgeI
AhdI AscI AseI AsnI Asp700I
Asp718I AspEI AviII BbsI BbvCI
BcgI BclI BfrI BglII BpuAI
BsaBI BseAI BsiWI BsmBI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrGI
BssHII BstBI ClaI CpoI DrdI
Eam1105I EarI EcoRI EcoRV FbaI
FspI HpaI KpnI Ksp632I MamI
MboII MfeI MluI MroI MunI
NdeI NheI NotI NspV PacI
PinAI PmeI PshAI PshBI PvuI
RcaI RsrII SalI SapI SbfI
ScaI SexAI SfuI SgrAI SnaBI
Sse8387I SspBI SwaI XbaI XmnI
###################################
# ONE CUT ENZYMES ( 32 enzymes) #
###################################
AclI 3310 AACGTT AA^CGTT
AcsI 3888 AAATTT [GA]^AATT[CT]
Aor51HI 562 AGCGCT AGC^GCT
ApoI 3888 AAATTT [GA]^AATT[CT]
BamHI 269 GGATCC G^GATCC
BciVI 2360 GTATCC GTATCC(6/5)
BsaWI 1651 ACCGGA [AT]^CCGG[AT]
BsmI 652 GAATGC GAATGC(1/-1)
BsrDI 1362 GCAATG GCAATG(2/0)
BstEII 740 GGTCACC G^GT[ACGT]ACC
BstPI 740 GGTCACC G^GT[ACGT]ACC
EagI 2681 CGGCCG C^GGCCG
EclXI 2681 CGGCCG C^GGCCG
Eco47III 562 AGCGCT AGC^GCT
Eco52I 2681 CGGCCG C^GGCCG
EcoNI 3334 CCTGGGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 740 GGTCACC G^GT[ACGT]ACC
EcoT22I 2653 ATGCAT ATGCA^T
FseI 2642 GGCCGGCC GGCCGG^CC
HindIII 3787 AAGCTT A^AGCTT
KspI 34 CCGCGG CCGC^GG
NruI 149 TCGCGA TCG^CGA
NsiI 2653 ATGCAT ATGCA^T
PaeR7I 525 CTCGAG C^TCGAG
Psp1406I 3310 AACGTT AA^CGTT
SacI 258 GAGCTC GAGCT^C
SacII 34 CCGCGG CCGC^GG
SfiI 522 GGCCTCGAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI 525 CTCGAG C^T[CT][GA]AG
SpeI 3850 ACTAGT A^CTAGT
SspI 3970 AATATT AAT^ATT
XhoI 525 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AspI 159 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
2157 GACGGTGTC
AvrII 2273 CCTAGG C^CTAGG
3571 CCTAGG
BalI 441 TGGCCA TGG^CCA
538 TGGCCA
BbeI 1001 GGCGCC GGCGC^C
3072 GGCGCC
BbrPI 2522 CACGTG CAC^GTG
3255 CACGTG
BlnI 2273 CCTAGG C^CTAGG
3571 CCTAGG
BlpI 2716 GCTCAGC GC^T[ACGT]AGC
2956 GCTGAGC
Bpu1102I 2716 GCTCAGC GC^T[ACGT]AGC
2956 GCTGAGC
BsiEI 2681 CGGCCG CG[GA][CT]^CG
2688 CGGTCG
BssSI 699 CACGAG CACGAG(-5/-1)
2165 CACGAG
Bst1107I 3876 GTATAC GTA^TAC
4017 GTATAC
BstYI 269 GGATCC [GA]^GATC[CT]
2048 AGATCC
BstZ17I 3876 GTATAC GTA^TAC
4017 GTATAC
CelII 2716 GCTCAGC GC^T[ACGT]AGC
2956 GCTGAGC
DraI 3421 TTTAAA TTT^AAA
3913 TTTAAA
DraIII 1619 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
2007 CACTGAGTG
HincII 341 GTCAAC GT[CT]^[GA]AC
1629 GTTGAC
HindII 341 GTCAAC GT[CT]^[GA]AC
1629 GTTGAC
KasI 1001 GGCGCC G^GCGCC
3072 GGCGCC
MflI 269 GGATCC [GA]^GATC[CT]
2048 AGATCC
MluNI 441 TGGCCA TGG^CCA
538 TGGCCA
MscI 441 TGGCCA TGG^CCA
538 TGGCCA
NarI 1001 GGCGCC GG^CGCC
3072 GGCGCC
PflFI 159 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
2157 GACGGTGTC
PmaCI 2522 CACGTG CAC^GTG
3255 CACGTG
PmlI 2522 CACGTG CAC^GTG
3255 CACGTG
SfoI 1001 GGCGCC GGC^GCC
3072 GGCGCC
StuI 919 AGGCCT AGG^CCT
1799 AGGCCT
Tth111I 159 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
2157 GACGGTGTC
XhoII 269 GGATCC [GA]^GATC[CT]
2048 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 121 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AflIII AluI Alw44I AlwI AlwNI
AocI ApaI ApaLI AspHI AvaI
AvaII BanI BanII BbvI BcnI
BfaI BglI BmyI BpmI BsaAI
BsaHI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrFI
BsrI BssKI BstAPI BstF5I BstNI
BstUI BstXI Bsu36I Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI Eco81I EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
MaeI MaeII MaeIII MboI MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NciI NcoI
NdeII NgoMIV NlaIII NlaIV NspI
PflMI PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI SphI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XmaCI
XmaI