##################################
# NO CUT ENZYMES ( 63 enzymes) #
##################################
AatII AccIII AclI AgeI AscI
AspI AvrII BalI BamHI BclI
BglII BlnI BsaBI BseAI BsiWI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrGI BssHII Bst1107I BstZ17I ClaI
EcoRI EcoRV EcoT22I FbaI HindIII
HpaI MamI MfeI MluI MluNI
MroI MscI MunI NcoI NdeI
NheI NruI NsiI PacI PflFI
PinAI PmeI Psp1406I RcaI SacI
SapI SbfI ScaI SexAI SfiI
SnaBI SphI Sse8387I SspBI StuI
SwaI Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AflII 2538 CTTAAG C^TTAAG
AhdI 3234 GACAGCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AseI 464 ATTAAT AT^TAAT
AsnI 464 ATTAAT AT^TAAT
Asp700I 909 GAAGCGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 3234 GACAGCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 970 TGCGCA TGC^GCA
BciVI 489 GTATCC GTATCC(6/5)
BfrI 2538 CTTAAG C^TTAAG
BglI 192 GCCACTGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaWI 1808 TCCGGT [AT]^CCGG[AT]
BssSI 1793 CACGAG CACGAG(-5/-1)
BstBI 2751 TTCGAA TT^CGAA
BstEII 951 GGTGACC G^GT[ACGT]ACC
BstPI 951 GGTGACC G^GT[ACGT]ACC
DraIII 4239 CACCCCGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1542 GACTCAGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 4948 CGGCCG C^GGCCG
Eam1105I 3234 GACAGCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 4840 GAAGAG CTCTTC(1/4)
EclXI 4948 CGGCCG C^GGCCG
Eco52I 4948 CGGCCG C^GGCCG
EcoO65I 951 GGTGACC G^GT[ACGT]ACC
EcoT14I 3673 CCTTGG C^C[AT][AT]GG
FseI 264 GGCCGGCC GGCCGG^CC
FspI 970 TGCGCA TGC^GCA
Ksp632I 4840 GAAGAG CTCTTC(1/4)
NotI 4947 GCGGCCGC GC^GGCCGC
NspV 2751 TTCGAA TT^CGAA
PaeR7I 258 CTCGAG C^TCGAG
PflMI 2921 CCACAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 2862 GACATCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 464 ATTAAT AT^TAAT
PvuI 3950 CGATCG CGAT^CG
SalI 1551 GTCGAC G^TCGAC
SfuI 2751 TTCGAA TT^CGAA
SgrAI 304 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 3653 CCCGGG CCC^GGG
SpeI 3353 ACTAGT A^CTAGT
StyI 3673 CCTTGG C^C[AT][AT]GG
Van91I 2921 CCACAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 258 CTCGAG C^TCGAG
XmaCI 3653 CCCGGG C^CCGGG
XmaI 3653 CCCGGG C^CCGGG
XmnI 909 GAAGCGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 38 enzymes) #
###################################
Acc65I 2735 GGTACC G^GTACC
2777 GGTACC
AccI 1551 GTCGAC GT^[AC][GT]AC
4383 GTCTAC
AcsI 525 GAATTT [GA]^AATT[CT]
2896 AAATTC
Alw44I 1500 GTGCAC G^TGCAC
4939 GTGCAC
AocI 1883 CCTGAGG CC^T[ACGT]AGG
4474 CCTCAGG
Aor51HI 2000 AGCGCT AGC^GCT
3314 AGCGCT
ApaLI 1500 GTGCAC G^TGCAC
4939 GTGCAC
ApoI 525 GAATTT [GA]^AATT[CT]
2896 AAATTC
Asp718I 2735 GGTACC G^GTACC
2777 GGTACC
BbeI 401 GGCGCC GGCGC^C
4759 GGCGCC
BbrPI 2160 CACGTG CAC^GTG
2819 CACGTG
BcgI 1634 CGACCACTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3992 CGAACTCCTTGC
BlpI 1734 GCTAAGC GC^T[ACGT]AGC
4601 GCTCAGC
Bpu1102I 1734 GCTAAGC GC^T[ACGT]AGC
4601 GCTCAGC
BsaAI 2160 CACGTG [CT]AC^GT[GA]
2819 CACGTG
BsiEI 3950 CGATCG CG[GA][CT]^CG
4948 CGGCCG
BsmBI 3561 GAGACG CGTCTC(1/5)
4847 GAGACG
BsrDI 2710 CATTGC GCAATG(2/0)
4630 CATTGC
BstXI 3805 CCAGTGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4411 CCAAAGAGCTGG
Bsu36I 1883 CCTGAGG CC^T[ACGT]AGG
4474 CCTCAGG
CelII 1734 GCTAAGC GC^T[ACGT]AGC
4601 GCTCAGC
CpoI 128 CGGACCG CG^G[AT]CCG
140 CGGACCG
DraI 2283 TTTAAA TTT^AAA
3140 TTTAAA
Eco47III 2000 AGCGCT AGC^GCT
3314 AGCGCT
Eco81I 1883 CCTGAGG CC^T[ACGT]AGG
4474 CCTCAGG
EcoNI 3224 CCTCCTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4121 CCTCTCAGAGG
HincII 1551 GTCGAC GT[CT]^[GA]AC
4625 GTCAAC
HindII 1551 GTCGAC GT[CT]^[GA]AC
4625 GTCAAC
KasI 401 GGCGCC G^GCGCC
4759 GGCGCC
KpnI 2735 GGTACC GGTAC^C
2777 GGTACC
MslI 2514 CACATAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
3545 CACAGAAGTG
NarI 401 GGCGCC GG^CGCC
4759 GGCGCC
NspI 988 ACATGC [GA]CATG^[CT]
2629 GCATGT
PmaCI 2160 CACGTG CAC^GTG
2819 CACGTG
PmlI 2160 CACGTG CAC^GTG
2819 CACGTG
PvuII 1413 CAGCTG CAG^CTG
3571 CAGCTG
RsrII 128 CGGACCG CG^G[AT]CCG
140 CGGACCG
SfoI 401 GGCGCC GGC^GCC
4759 GGCGCC
#########################################
# 3 OR MORE CUT ENZYMES ( 112 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI AlwNI ApaI AspHI
AvaI AvaII BanI BanII BbsI
BbvCI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaHI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
BspMI BsrBI BsrFI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EcoO109I
EcoRII FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KspI MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MspA1I
MspI MvaI MvnI MwoI NaeI
NciI NdeII NgoMIV NlaIII NlaIV
PleI PpuMI PstI RsaI SacII
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI XhoII