##################################
# NO CUT ENZYMES ( 59 enzymes) #
##################################
AclI AflII AgeI Alw44I Aor51HI
ApaLI AscI AseI AsnI AspI
AviII AvrII BbvCI BciVI BfrI
BglII BlnI BsiWI BspHI Bst1107I
BstAPI BstBI BstZ17I CpoI Eco47III
EcoRV EcoT22I FspI MfeI MluI
MunI NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmeI PshBI Psp1406I PvuI
RcaI RsrII SalI ScaI SfuI
SgrAI SnaBI SpeI SphI SwaI
Tth111I Van91I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AatII 2360 GACGTC GACGT^C
Acc65I 4171 GGTACC G^GTACC
AhdI 3890 GACCCGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp718I 4171 GGTACC G^GTACC
AspEI 3890 GACCCGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 1248 GGATCC G^GATCC
BbrPI 1739 CACGTG CAC^GTG
BcgI 185 GCAGTGGACTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 2262 TGATCA T^GATCA
BlpI 3122 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 3122 GCTAAGC GC^T[ACGT]AGC
BsgI 3258 GTGCAG GTGCAG(16/14)
BsiEI 3956 CGGCCG CG[GA][CT]^CG
BsmI 2768 GAATGC GAATGC(1/-1)
BspDI 1580 ATCGAT AT^CGAT
BssSI 996 CTCGTG CACGAG(-5/-1)
BstEII 1222 GGTGACC G^GT[ACGT]ACC
BstPI 1222 GGTGACC G^GT[ACGT]ACC
CelII 3122 GCTAAGC GC^T[ACGT]AGC
ClaI 1580 ATCGAT AT^CGAT
DraI 3546 TTTAAA TTT^AAA
DraIII 2197 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2063 GACGAGATGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 3956 CGGCCG C^GGCCG
Eam1105I 3890 GACCCGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 3956 CGGCCG C^GGCCG
Eco52I 3956 CGGCCG C^GGCCG
EcoO65I 1222 GGTGACC G^GT[ACGT]ACC
EcoRI 4758 GAATTC G^AATTC
FbaI 2262 TGATCA T^GATCA
FseI 1073 GGCCGGCC GGCCGG^CC
HincII 4436 GTTAAC GT[CT]^[GA]AC
HindII 4436 GTTAAC GT[CT]^[GA]AC
HpaI 4436 GTTAAC GTT^AAC
KpnI 4171 GGTACC GGTAC^C
NdeI 4686 CATATG CA^TATG
PmaCI 1739 CACGTG CAC^GTG
PmlI 1739 CACGTG CAC^GTG
PshAI 1186 GACCCCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 293 GAAGAGC GCTCTTC(1/4)
SbfI 1481 CCTGCAGG CCTGCA^GG
SexAI 1470 ACCTGGT A^CC[AT]GGT
SfiI 2965 GGCCCCCACGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Sse8387I 1481 CCTGCAGG CCTGCA^GG
SspI 4737 AATATT AAT^ATT
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccI 1673 GTCTAC GT^[AC][GT]AC
4671 GTAGAC
AflIII 2160 ACATGT A^C[GA][CT]GT
4296 ACATGT
Asp700I 3612 GAATTTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4708 GAAGTGTTTC
BbeI 1440 GGCGCC GGCGC^C
2554 GGCGCC
BsaAI 1739 CACGTG [CT]AC^GT[GA]
2081 TACGTG
Bsp1407I 4010 TGTACA T^GTACA
4299 TGTACA
BspLU11I 2160 ACATGT A^CATGT
4296 ACATGT
BsrDI 486 CATTGC GCAATG(2/0)
969 CATTGC
BsrGI 4010 TGTACA T^GTACA
4299 TGTACA
BssHII 2216 GCGCGC G^CGCGC
3914 GCGCGC
BstXI 378 CCAAGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3080 CCAGAGGCCTGG
BstYI 1248 GGATCC [GA]^GATC[CT]
2367 AGATCC
HindIII 3410 AAGCTT A^AGCTT
3988 AAGCTT
KasI 1440 GGCGCC G^GCGCC
2554 GGCGCC
KspI 2596 CCGCGG CCGC^GG
2865 CCGCGG
MflI 1248 GGATCC [GA]^GATC[CT]
2367 AGATCC
NaeI 1074 GCCGGC GCC^GGC
1443 GCCGGC
NarI 1440 GGCGCC GG^CGCC
2554 GGCGCC
NcoI 2043 CCATGG C^CATGG
3622 CCATGG
NgoMIV 1074 GCCGGC G^CCGGC
1443 GCCGGC
PvuII 1945 CAGCTG CAG^CTG
2233 CAGCTG
SacI 417 GAGCTC GAGCT^C
709 GAGCTC
SacII 2596 CCGCGG CCGC^GG
2865 CCGCGG
SfoI 1440 GGCGCC GGC^GCC
2554 GGCGCC
SspBI 4010 TGTACA T^GTACA
4299 TGTACA
TfiI 2849 GAATC G^A[AT]TC
4286 GATTC
XhoII 1248 GGATCC [GA]^GATC[CT]
2367 AGATCC
XmnI 3612 GAATTTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4708 GAAGTGTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 126 enzymes) #
#########################################
AccII AccIII AciI AcsI AcyI
AfaI AluI AlwI AlwNI AocI
ApaI ApoI AspHI AvaI AvaII
BalI BanI BanII BbsI BbvI
BcnI BfaI BglI BmyI BpmI
BpuAI BsaBI BsaHI BsaI BsaJI
BsaWI BseAI BseRI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
Bsp1286I BspEI BspMI BsrBI BsrFI
BsrI BssKI BstF5I BstNI BstUI
Bsu36I Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MamI
MboI MboII MluNI MnlI MroI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PpuMI
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI SmlI StuI
StyI TaqI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XmaCI
XmaI