##################################
# NO CUT ENZYMES ( 61 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI Aor51HI AseI AsnI Asp718I
BcgI BciVI BfrI BsaWI BseAI
BsmI BspDI BspEI BspHI Bst1107I
BstBI BstZ17I ClaI CpoI DraI
Eco47III EcoRV EcoT22I FseI HpaI
KpnI MfeI MluI MroI MunI
NdeI NheI NruI NsiI NspV
PacI PaeR7I PinAI PmeI PshBI
Psp1406I PvuI RcaI RsrII SbfI
ScaI SexAI SfuI SgrAI SnaBI
SpeI Sse8387I SspI SwaI XbaI
XhoI
###################################
# ONE CUT ENZYMES ( 25 enzymes) #
###################################
AscI 73 GGCGCGCC GG^CGCGCC
Asp700I 744 GAACGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 2907 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 2441 GGATCC G^GATCC
BglII 5369 AGATCT A^GATCT
BsaI 1186 GAGACC GGTCTC(1/5)
BsiWI 3552 CGTACG C^GTACG
BspLU11I 1175 ACATGT A^CATGT
BsrDI 2858 CATTGC GCAATG(2/0)
BssHII 74 GCGCGC G^CGCGC
BstEII 1080 GGTCACC G^GT[ACGT]ACC
BstPI 1080 GGTCACC G^GT[ACGT]ACC
DrdI 1281 GACGGCAGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 4485 CCTGGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1080 GGTCACC G^GT[ACGT]ACC
EcoRI 1999 GAATTC G^AATTC
HindIII 1238 AAGCTT A^AGCTT
MseI 5614 TTAA T^TAA
NotI 904 GCGGCCGC GC^GGCCGC
PflFI 2907 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
SapI 1862 GCTCTTC GCTCTTC(1/4)
SphI 4541 GCATGC GCATG^C
Tru9I 5614 TTAA T^TAA
Tth111I 2907 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 744 GAACGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AcsI 439 AAATTC [GA]^AATT[CT]
1999 GAATTC
ApoI 439 AAATTC [GA]^AATT[CT]
1999 GAATTC
AviII 419 TGCGCA TGC^GCA
1094 TGCGCA
BbeI 3709 GGCGCC GGCGC^C
4213 GGCGCC
BbsI 4874 GAAGAC GAAGAC(2/6)
2887 GTCTTC
BbvCI 1491 GCTGAGG CCTCAGC(-5/-2)
5183 GCTGAGG
BclI 1781 TGATCA T^GATCA
2477 TGATCA
BpuAI 4874 GAAGAC GAAGAC(2/6)
2887 GTCTTC
BsaBI 123 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4293 GATGCGGATC
Bsp1407I 1645 TGTACA T^GTACA
4157 TGTACA
BsrGI 1645 TGTACA T^GTACA
4157 TGTACA
DraIII 346 CACGAGGTG CAC[ACGT][ACGT][ACGT]^GTG
3027 CACCATGTG
FbaI 1781 TGATCA T^GATCA
2477 TGATCA
FspI 419 TGCGCA TGC^GCA
1094 TGCGCA
KasI 3709 GGCGCC G^GCGCC
4213 GGCGCC
MamI 123 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4293 GATGCGGATC
NarI 3709 GGCGCC GG^CGCC
4213 GGCGCC
SacI 280 GAGCTC GAGCT^C
2404 GAGCTC
SfiI 2802 GGCCACAGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
5264 GGCCTCTCTGGCC
SfoI 3709 GGCGCC GGC^GCC
4213 GGCGCC
SmlI 4400 CTCAAG C^T[CT][GA]AG
4780 CTTGAG
SspBI 1645 TGTACA T^GTACA
4157 TGTACA
StuI 2105 AGGCCT AGG^CCT
4963 AGGCCT
Tsp509I 440 AATT ^AATT
2000 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 148 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AflIII AhdI AluI Alw44I AlwI
AlwNI AocI ApaI ApaLI AspEI
AspHI AvaI AvaII AvrII BalI
BanI BanII BbrPI BbvI BcnI
BfaI BglI BlnI BlpI BmyI
BpmI Bpu1102I BsaAI BsaHI BsaJI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrFI BsrI
BssKI BssSI BstAPI BstF5I BstNI
BstUI BstXI BstYI Bsu36I Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EagI
Eam1105I EarI EclXI Eco52I Eco81I
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
KspI MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NciI NcoI NdeII
NgoMIV NlaIII NlaIV NspI PflMI
PleI PmaCI PmlI PpuMI PshAI
PstI PvuII RsaI SacII SalI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI StyI TaqI TfiI TseI
Tsp45I TspRI TthHB8I Van91I XcmI
XhoII XmaCI XmaI