##################################
# NO CUT ENZYMES ( 77 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
AhdI ApaI AscI Asp718I AspEI
AspI AvrII BamHI BanI BanII
BbeI BbrPI BglI BlnI BsaAI
BsaWI BseAI BsiEI BsiWI Bsp1407I
BspDI BspEI BsrBI BsrDI BsrGI
BssHII BstEII BstPI ClaI CpoI
DrdI EagI Eam1105I EclXI Eco52I
EcoNI EcoO65I EcoT22I HpaI KasI
KpnI KspI MluI MroI NarI
NotI NruI NsiI PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI Psp1406I PvuI RsrII SacI
SacII SalI SbfI SexAI SfiI
SfoI SgrAI SnaBI Sse8387I SspBI
Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AcyI 38 GACGCC G[GA]^CG[CT]C
AflIII 4341 ACATGT A^C[GA][CT]GT
Alw44I 4696 GTGCAC G^TGCAC
AlwNI 264 CAGCGTCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1410 CCTGAGG CC^T[ACGT]AGG
Aor51HI 56 AGCGCT AGC^GCT
ApaLI 4696 GTGCAC G^TGCAC
Asp700I 1790 GAATTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 199 TGCGCA TGC^GCA
BbvCI 5129 GCTGAGG CCTCAGC(-5/-2)
BcgI 5135 GCAGGAGAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 4416 GTATCC GTATCC(6/5)
BclI 3522 TGATCA T^GATCA
BglII 1950 AGATCT A^GATCT
BsaHI 38 GACGCC G[GA]^CG[CT]C
BseRI 3183 GAGGAG GAGGAG(10/8)
BsgI 313 CTGCAC GTGCAG(16/14)
BspLU11I 4341 ACATGT A^CATGT
BstBI 2066 TTCGAA TT^CGAA
Bsu36I 1410 CCTGAGG CC^T[ACGT]AGG
Eco47III 56 AGCGCT AGC^GCT
Eco81I 1410 CCTGAGG CC^T[ACGT]AGG
EcoRI 1148 GAATTC G^AATTC
EcoRV 1232 GATATC GAT^ATC
FbaI 3522 TGATCA T^GATCA
FseI 227 GGCCGGCC GGCCGG^CC
FspI 199 TGCGCA TGC^GCA
HaeII 56 AGCGCT [GA]GCGC^[CT]
Hin1I 38 GACGCC G[GA]^CG[CT]C
MfeI 3095 CAATTG C^AATTG
MspA1I 505 CAGCTG C[AC]G^C[GT]G
MunI 3095 CAATTG C^AATTG
NheI 760 GCTAGC G^CTAGC
NspV 2066 TTCGAA TT^CGAA
PvuII 505 CAGCTG CAG^CTG
SapI 52 GAAGAGC GCTCTTC(1/4)
ScaI 3206 AGTACT AGT^ACT
SfuI 2066 TTCGAA TT^CGAA
SpeI 1825 ACTAGT A^CTAGT
SphI 1076 GCATGC GCATG^C
StuI 4277 AGGCCT AGG^CCT
SwaI 3158 ATTTAAAT ATTT^AAAT
XbaI 30 TCTAGA T^CTAGA
XmnI 1790 GAATTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 39 enzymes) #
###################################
AccII 80 CGCG CG^CG
154 CGCG
AflII 702 CTTAAG C^TTAAG
991 CTTAAG
AvaI 125 CCCGGG C^[CT]CG[GA]G
193 CCCGGG
BalI 4837 TGGCCA TGG^CCA
5038 TGGCCA
BbsI 1109 GAAGAC GAAGAC(2/6)
1405 GAAGAC
BfrI 702 CTTAAG C^TTAAG
991 CTTAAG
BlpI 1632 GCTTAGC GC^T[ACGT]AGC
2300 GCTTAGC
Bpu1102I 1632 GCTTAGC GC^T[ACGT]AGC
2300 GCTTAGC
BpuAI 1109 GAAGAC GAAGAC(2/6)
1405 GAAGAC
BsaI 1062 GAGACC GGTCTC(1/5)
5028 GAGACC
BsoBI 125 CCCGGG C^[CT]CG[GA]G
193 CCCGGG
BspHI 2037 TCATGA T^CATGA
4376 TCATGA
BsrFI 228 GCCGGC [GA]^CCGG[CT]
4945 GCCGGC
BssSI 220 CACGAG CACGAG(-5/-1)
3657 CACGAG
Bst1107I 3945 GTATAC GTA^TAC
4404 GTATAC
BstUI 80 CGCG CG^CG
154 CGCG
BstXI 2156 CCAAGTCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5114 CCAGCTACTTGG
BstZ17I 3945 GTATAC GTA^TAC
4404 GTATAC
CelII 1632 GCTTAGC GC^T[ACGT]AGC
2300 GCTTAGC
Cfr10I 228 GCCGGC [GA]^CCGG[CT]
4945 GCCGGC
DraII 251 AGGACCC [GA]G^G[ACGT]CC[CT]
343 GGGACCT
DraIII 1246 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
3366 CACTAGGTG
EaeI 4837 TGGCCA [CT]^GGCC[GA]
5038 TGGCCA
EcoO109I 251 AGGACCC [GA]G^G[ACGT]CC[CT]
343 GGGACCT
MluNI 4837 TGGCCA TGG^CCA
5038 TGGCCA
MscI 4837 TGGCCA TGG^CCA
5038 TGGCCA
MvnI 80 CGCG CG^CG
154 CGCG
NaeI 228 GCCGGC GCC^GGC
4945 GCCGGC
NcoI 1985 CCATGG C^CATGG
4765 CCATGG
NdeI 1769 CATATG CA^TATG
4307 CATATG
NgoMIV 228 GCCGGC G^CCGGC
4945 GCCGGC
PflMI 1066 CCATATTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2596 CCAAAGTATGG
PpuMI 251 AGGACCC [GA]G^G[AT]CC[CT]
343 GGGACCT
RcaI 2037 TCATGA T^CATGA
4376 TCATGA
SmaI 125 CCCGGG CCC^GGG
193 CCCGGG
Van91I 1066 CCATATTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2596 CCAAAGTATGG
XcmI 1360 CCAAAGATGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4614 CCATGAACAAGTTGG
XmaCI 125 CCCGGG C^CCGGG
193 CCCGGG
XmaI 125 CCCGGG C^CCGGG
193 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 98 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI ApoI AseI AsnI AspHI
AvaII BbvI BcnI BfaI BmyI
BpmI BsaBI BsaJI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsmI
Bsp1286I BspMI BsrI BssKI BstAPI
BstF5I BstNI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraI DsaI
EarI EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HincII HindII HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MamI MboI MboII
MflI MnlI MseI MslI MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PshBI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII