##################################
# NO CUT ENZYMES ( 93 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AgeI Alw44I Aor51HI ApaLI
AscI AseI AsnI Asp700I Asp718I
AviII AvrII BbeI BbrPI BcgI
BfrI BglI BglII BlnI BsaAI
BsaHI BseAI BsiEI BsiWI BsmI
Bsp1407I BspEI BsrDI BsrGI Bst1107I
BstAPI BstBI BstZ17I CpoI EagI
EclXI Eco47III Eco52I EcoRI EcoT22I
FseI FspI Hin1I HpaI KasI
KpnI KspI MluI MroI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflMI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RsrII SacI
SacII SalI SbfI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SspBI Van91I XbaI XhoI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AccI 2404 GTCTAC GT^[AC][GT]AC
AflIII 2180 ACATGT A^C[GA][CT]GT
AocI 2081 CCTCAGG CC^T[ACGT]AGG
ApaI 1997 GGGCCC GGGCC^C
BalI 4495 TGGCCA TGG^CCA
BciVI 1267 GTATCC GTATCC(6/5)
BclI 1209 TGATCA T^GATCA
BsaBI 425 GATGCCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 778 TCCGGT [AT]^CCGG[AT]
BspDI 132 ATCGAT AT^CGAT
BspLU11I 2180 ACATGT A^CATGT
BsrFI 4488 ACCGGC [GA]^CCGG[CT]
BssHII 4556 GCGCGC G^CGCGC
BssSI 404 CACGAG CACGAG(-5/-1)
BstEII 1367 GGTCACC G^GT[ACGT]ACC
BstPI 1367 GGTCACC G^GT[ACGT]ACC
Bsu36I 2081 CCTCAGG CC^T[ACGT]AGG
Cfr10I 4488 ACCGGC [GA]^CCGG[CT]
ClaI 132 ATCGAT AT^CGAT
DraIII 2305 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 4336 GACAGTTTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 2081 CCTCAGG CC^T[ACGT]AGG
EcoNI 472 CCTACAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1367 GGTCACC G^GT[ACGT]ACC
EcoRV 569 GATATC GAT^ATC
FbaI 1209 TGATCA T^GATCA
HgaI 2225 GCGTC GACGC(5/10)
HindIII 353 AAGCTT A^AGCTT
MamI 425 GATGCCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1626 CAATTG C^AATTG
MluNI 4495 TGGCCA TGG^CCA
MscI 4495 TGGCCA TGG^CCA
MunI 1626 CAATTG C^AATTG
PshAI 3592 GACTGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SexAI 3853 ACCAGGT A^CC[AT]GGT
SphI 3057 GCATGC GCATG^C
SspI 3873 AATATT AAT^ATT
SwaI 2065 ATTTAAAT ATTT^AAAT
XcmI 1662 CCAAAGACATTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AccII 2113 CGCG CG^CG
4557 CGCG
AhdI 1232 GACTTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2580 GACAAATTGTC
AspEI 1232 GACTTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2580 GACAAATTGTC
AspHI 2148 GAGCAC G[AT]GC[AT]^C
2512 GAGCAC
BlpI 410 GCTAAGC GC^T[ACGT]AGC
1453 GCTGAGC
Bpu1102I 410 GCTAAGC GC^T[ACGT]AGC
1453 GCTGAGC
BsiHKAI 2148 GAGCAC G[AT]GC[AT]^C
2512 GAGCAC
BsrBI 2544 CCGCTC CCGCTC(-3/-3)
4574 CCGCTC
BstUI 2113 CGCG CG^CG
4557 CGCG
CelII 410 GCTAAGC GC^T[ACGT]AGC
1453 GCTGAGC
EaeI 2996 TGGCCG [CT]^GGCC[GA]
4495 TGGCCA
Eam1105I 1232 GACTTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2580 GACAAATTGTC
HincII 1918 GTTGAC GT[CT]^[GA]AC
2760 GTTGAC
HindII 1918 GTTGAC GT[CT]^[GA]AC
2760 GTTGAC
MvnI 2113 CGCG CG^CG
4557 CGCG
NspI 2180 ACATGT [GA]CATG^[CT]
3057 GCATGC
StuI 894 AGGCCT AGG^CCT
2136 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 109 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AspI AvaI AvaII
BamHI BanI BanII BbsI BbvCI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaI BsaJI BseRI BsgI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I BspHI BspMI BsrI
BssKI BstF5I BstNI BstXI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraII DsaI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NciI NcoI
NdeII NlaIII NlaIV PflFI PleI
PpuMI PstI PvuII RcaI RsaI
SapI Sau3AI Sau96I ScaI ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Tth111I TthHB8I XhoII