##################################
# NO CUT ENZYMES ( 68 enzymes) #
##################################
Acc65I AccI AccIII AclI AflII
AhdI Alw44I Aor51HI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
BamHI BbrPI BfrI BglII BsaAI
BsaBI BseAI BsgI BsiWI BsmI
BspEI Bst1107I BstAPI BstBI BstYI
BstZ17I CpoI Eam1105I Eco47III EcoRI
EcoT22I FseI HindIII KpnI MamI
MfeI MflI MluI MroI MunI
NheI NotI NruI NsiI NspV
PacI PmaCI PmeI PmlI PshBI
Psp1406I RsrII SalI SapI SfiI
SfuI SgrAI SnaBI SpeI SspI
SwaI XhoII XmnI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AatII 3682 GACGTC GACGT^C
AcsI 2992 AAATTC [GA]^AATT[CT]
AgeI 2121 ACCGGT A^CCGGT
ApoI 2992 AAATTC [GA]^AATT[CT]
AspI 2011 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1526 TGCGCA TGC^GCA
AvrII 39 CCTAGG C^CTAGG
BalI 1827 TGGCCA TGG^CCA
BciVI 2574 GGATAC GTATCC(6/5)
BclI 246 TGATCA T^GATCA
BlnI 39 CCTAGG C^CTAGG
BspHI 434 TCATGA T^CATGA
BspLU11I 4444 ACATGT A^CATGT
BssHII 44 GCGCGC G^CGCGC
DraI 3431 TTTAAA TTT^AAA
DrdI 2232 GACTAGCCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 3106 GATATC GAT^ATC
FbaI 246 TGATCA T^GATCA
FspI 1526 TGCGCA TGC^GCA
HincII 4342 GTTAAC GT[CT]^[GA]AC
HindII 4342 GTTAAC GT[CT]^[GA]AC
HpaI 4342 GTTAAC GTT^AAC
MluNI 1827 TGGCCA TGG^CCA
MscI 1827 TGGCCA TGG^CCA
NcoI 1550 CCATGG C^CATGG
NdeI 4314 CATATG CA^TATG
PaeR7I 2776 CTCGAG C^TCGAG
PflFI 2011 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 2121 ACCGGT A^CCGGT
PvuI 2498 CGATCG CGAT^CG
RcaI 434 TCATGA T^CATGA
ScaI 2176 AGTACT AGT^ACT
SphI 1523 GCATGC GCATG^C
TfiI 1693 GATTC G^A[AT]TC
Tth111I 2011 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 3505 TCTAGA T^CTAGA
XhoI 2776 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AflIII 4444 ACATGT A^C[GA][CT]GT
5001 ACGTGT
AlwI 1812 GATCC GGATC(4/5)
431 GGATC
BbsI 1356 GAAGAC GAAGAC(2/6)
2109 GAAGAC
BlpI 4099 GCTGAGC GC^T[ACGT]AGC
4714 GCTCAGC
Bpu1102I 4099 GCTGAGC GC^T[ACGT]AGC
4714 GCTCAGC
BpuAI 1356 GAAGAC GAAGAC(2/6)
2109 GAAGAC
Bsp1407I 4310 TGTACA T^GTACA
4466 TGTACA
BsrDI 932 GCAATG GCAATG(2/0)
4672 GCAATG
BsrGI 4310 TGTACA T^GTACA
4466 TGTACA
BstEII 384 GGTGACC G^GT[ACGT]ACC
3891 GGTCACC
BstPI 384 GGTGACC G^GT[ACGT]ACC
3891 GGTCACC
CelII 4099 GCTGAGC GC^T[ACGT]AGC
4714 GCTCAGC
DraIII 2300 CACGGGGTG CAC[ACGT][ACGT][ACGT]^GTG
3883 CACACAGTG
EagI 112 CGGCCG C^GGCCG
1496 CGGCCG
EclXI 112 CGGCCG C^GGCCG
1496 CGGCCG
Eco52I 112 CGGCCG C^GGCCG
1496 CGGCCG
EcoNI 147 CCTCTGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2472 CCTCTCGGAGG
EcoO65I 384 GGTGACC G^GT[ACGT]ACC
3891 GGTCACC
PshAI 3771 GACGACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3965 GACATGCGTC
SacI 1050 GAGCTC GAGCT^C
3878 GAGCTC
SbfI 1428 CCTGCAGG CCTGCA^GG
4685 CCTGCAGG
SexAI 185 ACCTGGT A^CC[AT]GGT
857 ACCTGGT
Sse8387I 1428 CCTGCAGG CCTGCA^GG
4685 CCTGCAGG
SspBI 4310 TGTACA T^GTACA
4466 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 129 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwNI AocI ApaI AspHI AvaI
AvaII BanI BanII BbeI BbvCI
BbvI BcgI BcnI BfaI BglI
BmyI BpmI BsaHI BsaI BsaJI
BsaWI BseRI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
Bsp1286I BspDI BspMI BsrBI BsrFI
BsrI BssKI BssSI BstF5I BstNI
BstUI BstXI Bsu36I Cac8I CfoI
Cfr10I Cfr13I ClaI DdeI DpnII
DraII DsaI EaeI EarI Eco81I
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI KasI Ksp632I KspI
MaeI MaeII MaeIII MboI MboII
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NarI
NciI NdeII NgoMIV NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII RsaI SacII Sau3AI Sau96I
ScrFI SfaNI SfcI SfoI SmaI
SmlI StuI StyI TaqI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XmaCI XmaI