##################################
# NO CUT ENZYMES ( 42 enzymes) #
##################################
Acc65I AflIII AgeI AscI Asp718I
AspI BcgI BsaBI BsiWI BspDI
BspLU11I Bst1107I BstBI BstZ17I ClaI
EcoT22I FseI HpaI KpnI KspI
MamI MfeI MluI MunI NotI
NruI NsiI NspV PacI PflFI
PinAI PmeI PvuI SacII SalI
SfiI SfuI SgrAI SnaBI SpeI
SwaI Tth111I
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AatII 1262 GACGTC GACGT^C
AccI 7684 GTAGAC GT^[AC][GT]AC
AccIII 5168 TCCGGA T^CCGGA
AclI 157 AACGTT AA^CGTT
AflII 4054 CTTAAG C^TTAAG
AhdI 1851 GACAGAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AseI 7111 ATTAAT AT^TAAT
AsnI 7111 ATTAAT AT^TAAT
AspEI 1851 GACAGAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 5891 TGCGCA TGC^GCA
BbeI 2977 GGCGCC GGCGC^C
BfrI 4054 CTTAAG C^TTAAG
BseAI 5168 TCCGGA T^CCGGA
BspEI 5168 TCCGGA T^CCGGA
BssHII 357 GCGCGC G^CGCGC
BstEII 4659 GGTCACC G^GT[ACGT]ACC
BstPI 4659 GGTCACC G^GT[ACGT]ACC
EagI 5940 CGGCCG C^GGCCG
Eam1105I 1851 GACAGAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 5940 CGGCCG C^GGCCG
Eco52I 5940 CGGCCG C^GGCCG
EcoO65I 4659 GGTCACC G^GT[ACGT]ACC
EcoRV 1663 GATATC GAT^ATC
FspI 5891 TGCGCA TGC^GCA
HincII 2709 GTTGAC GT[CT]^[GA]AC
HindII 2709 GTTGAC GT[CT]^[GA]AC
KasI 2977 GGCGCC G^GCGCC
MroI 5168 TCCGGA T^CCGGA
NarI 2977 GGCGCC GG^CGCC
NdeI 327 CATATG CA^TATG
NheI 4626 GCTAGC G^CTAGC
PshBI 7111 ATTAAT AT^TAAT
Psp1406I 157 AACGTT AA^CGTT
ScaI 5393 AGTACT AGT^ACT
SexAI 4802 ACCAGGT A^CC[AT]GGT
SfoI 2977 GGCGCC GGC^GCC
SspI 4075 AATATT AAT^ATT
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
Aor51HI 247 AGCGCT AGC^GCT
4652 AGCGCT
BamHI 710 GGATCC G^GATCC
736 GGATCC
BclI 1346 TGATCA T^GATCA
2339 TGATCA
BglII 559 AGATCT A^GATCT
1206 AGATCT
BsaWI 3854 TCCGGT [AT]^CCGG[AT]
5168 TCCGGA
BsiEI 3958 CGGTCG CG[GA][CT]^CG
5940 CGGCCG
BsmBI 1078 CGTCTC CGTCTC(1/5)
6845 GAGACG
Bsp1407I 2994 TGTACA T^GTACA
8133 TGTACA
BspHI 3660 TCATGA T^CATGA
4931 TCATGA
BsrDI 6740 CATTGC GCAATG(2/0)
8502 GCAATG
BsrGI 2994 TGTACA T^GTACA
8133 TGTACA
CpoI 3856 CGGTCCG CG^G[AT]CCG
5881 CGGACCG
DraIII 4116 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG
7267 CACTCAGTG
DrdI 6030 GACCCCGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
6220 GACACCCCTGTC
Eco47III 247 AGCGCT AGC^GCT
4652 AGCGCT
FbaI 1346 TGATCA T^GATCA
2339 TGATCA
HindIII 3450 AAGCTT A^AGCTT
8346 AAGCTT
NaeI 688 GCCGGC GCC^GGC
6005 GCCGGC
NcoI 150 CCATGG C^CATGG
608 CCATGG
NgoMIV 688 GCCGGC G^CCGGC
6005 GCCGGC
PaeR7I 5278 CTCGAG C^TCGAG
6842 CTCGAG
PshAI 221 GACGAGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
658 GACAAGTGTC
RcaI 3660 TCATGA T^CATGA
4931 TCATGA
RsrII 3856 CGGTCCG CG^G[AT]CCG
5881 CGGACCG
SbfI 2688 CCTGCAGG CCTGCA^GG
2718 CCTGCAGG
Sse8387I 2688 CCTGCAGG CCTGCA^GG
2718 CCTGCAGG
SspBI 2994 TGTACA T^GTACA
8133 TGTACA
XcmI 3983 CCATGAGCCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
6126 CCAAACACACTATGG
XhoI 5278 CTCGAG C^TCGAG
6842 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 150 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI Alw44I AlwI AlwNI AocI
ApaI ApaLI ApoI Asp700I AspHI
AvaI AvaII AvrII BalI BanI
BanII BbrPI BbsI BbvCI BbvI
BciVI BcnI BfaI BglI BlnI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaAI BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BspMI
BsrBI BsrFI BsrI BssKI BssSI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Bsu36I Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraI
DraII DsaI EaeI EarI Eco81I
EcoNI EcoO109I EcoRI EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PflMI PleI PmaCI PmlI
PpuMI PstI PvuII RsaI SacI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI SmlI SphI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XbaI XhoII XmaCI XmaI XmnI