##################################
# NO CUT ENZYMES ( 33 enzymes) #
##################################
Aor51HI AscI BbeI BbvCI BcgI
BsiWI BsmBI BssHII BssSI BstAPI
CpoI DraIII Eco47III FseI HaeII
KasI MluI NaeI NarI NgoMIV
NotI NruI PacI PmeI PvuI
RsrII SalI SbfI SfiI SfoI
SphI Sse8387I SwaI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AatII 1410 GACGTC GACGT^C
AclI 382 AACGTT AA^CGTT
AcyI 1410 GACGTC G[GA]^CG[CT]C
AhdI 3111 GACATTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 10399 GTGCAC G^TGCAC
ApaLI 10399 GTGCAC G^TGCAC
AspEI 3111 GACATTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 9174 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 11083 TGCGCA TGC^GCA
BamHI 7102 GGATCC G^GATCC
BbrPI 3074 CACGTG CAC^GTG
BsaHI 1410 GACGTC G[GA]^CG[CT]C
BspDI 5713 ATCGAT AT^CGAT
Bst1107I 6488 GTATAC GTA^TAC
BstEII 2703 GGTTACC G^GT[ACGT]ACC
BstPI 2703 GGTTACC G^GT[ACGT]ACC
BstZ17I 6488 GTATAC GTA^TAC
ClaI 5713 ATCGAT AT^CGAT
EagI 8485 CGGCCG C^GGCCG
Eam1105I 3111 GACATTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 8485 CGGCCG C^GGCCG
Eco52I 8485 CGGCCG C^GGCCG
EcoO65I 2703 GGTTACC G^GT[ACGT]ACC
FspI 11083 TGCGCA TGC^GCA
Hin1I 1410 GACGTC G[GA]^CG[CT]C
HpaI 7596 GTTAAC GTT^AAC
KspI 66 CCGCGG CCGC^GG
PaeR7I 808 CTCGAG C^TCGAG
PflFI 9174 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 3074 CACGTG CAC^GTG
PmlI 3074 CACGTG CAC^GTG
PshAI 4543 GACCACTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 382 AACGTT AA^CGTT
SacI 3270 GAGCTC GAGCT^C
SacII 66 CCGCGG CCGC^GG
SapI 3470 GAAGAGC GCTCTTC(1/4)
SgrAI 90 CACCGGTG C[GA]^CCGG[CT]G
SmaI 34 CCCGGG CCC^GGG
SnaBI 4568 TACGTA TAC^GTA
Tth111I 9174 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 808 CTCGAG C^TCGAG
XmaCI 34 CCCGGG C^CCGGG
XmaI 34 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AccI 6488 GTATAC GT^[AC][GT]AC
11567 GTCTAC
AccIII 8853 TCCGGA T^CCGGA
10805 TCCGGA
AflII 7268 CTTAAG C^TTAAG
8631 CTTAAG
AgeI 91 ACCGGT A^CCGGT
11775 ACCGGT
BalI 8653 TGGCCA TGG^CCA
12089 TGGCCA
BcnI 34 CCCGG CC^[GC]GG
35 CCGGG
BfrI 7268 CTTAAG C^TTAAG
8631 CTTAAG
BglI 272 GCCCCACTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
7182 GCCCACATGGC
BlpI 1632 GCTGAGC GC^T[ACGT]AGC
2217 GCTAAGC
Bpu1102I 1632 GCTGAGC GC^T[ACGT]AGC
2217 GCTAAGC
BsaBI 4719 GATGTAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5706 GATGATGATC
BsaI 19 GAGACC GGTCTC(1/5)
5396 GAGACC
BseAI 8853 TCCGGA T^CCGGA
10805 TCCGGA
BsiEI 574 CGACCG CG[GA][CT]^CG
8485 CGGCCG
BspEI 8853 TCCGGA T^CCGGA
10805 TCCGGA
BsrBI 9999 GAGCGG CCGCTC(-3/-3)
12063 GAGCGG
BstBI 6994 TTCGAA TT^CGAA
8998 TTCGAA
CelII 1632 GCTGAGC GC^T[ACGT]AGC
2217 GCTAAGC
EcoRI 5457 GAATTC G^AATTC
10839 GAATTC
HindIII 7724 AAGCTT A^AGCTT
8232 AAGCTT
MamI 4719 GATGTAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5706 GATGATGATC
MluNI 8653 TGGCCA TGG^CCA
12089 TGGCCA
MroI 8853 TCCGGA T^CCGGA
10805 TCCGGA
MscI 8653 TGGCCA TGG^CCA
12089 TGGCCA
NciI 34 CCCGG CC^[GC]GG
35 CCGGG
NdeI 2572 CATATG CA^TATG
8524 CATATG
NheI 2802 GCTAGC G^CTAGC
7974 GCTAGC
NspV 6994 TTCGAA TT^CGAA
8998 TTCGAA
PinAI 91 ACCGGT A^CCGGT
11775 ACCGGT
SexAI 8710 ACCAGGT A^CC[AT]GGT
9813 ACCTGGT
SfuI 6994 TTCGAA TT^CGAA
8998 TTCGAA
SpeI 4965 ACTAGT A^CTAGT
6365 ACTAGT
StuI 8762 AGGCCT AGG^CCT
9646 AGGCCT
XbaI 6501 TCTAGA T^CTAGA
6554 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 148 enzymes) #
#########################################
Acc65I AccII AciI AcsI AfaI
AflIII AluI AlwI AlwNI AocI
ApaI ApoI AseI AsnI Asp700I
Asp718I AspHI AvaI AvaII AvrII
BanI BanII BbsI BbvI BciVI
BclI BfaI BglII BlnI BmyI
BpmI BpuAI BsaAI BsaJI BsaWI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
Bsp1407I BspHI BspLU11I BspMI BsrDI
BsrFI BsrGI BsrI BssKI BstF5I
BstNI BstUI BstXI BstYI Bsu36I
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraI DraII DrdI DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoRII EcoRV EcoT14I EcoT22I FauI
FbaI Fnu4HI FokI HaeIII HapII
HgaI HhaI HinP1I HincII HindII
HinfI HpaII HphI ItaI KpnI
Ksp632I MaeI MaeII MaeIII MboI
MboII MfeI MflI MnlI MseI
MslI MspA1I MspI MunI MvaI
MvnI MwoI NcoI NdeII NlaIII
NlaIV NsiI NspI PflMI PleI
PpuMI PshBI PstI PvuII RcaI
RsaI Sau3AI Sau96I ScaI ScrFI
SfaNI SfcI SmlI SspBI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII XmnI