##################################
# NO CUT ENZYMES ( 60 enzymes) #
##################################
AatII AccI AclI AcsI AgeI
ApoI AscI AseI AsnI Asp700I
AspI BbrPI BsaBI Bsp1407I BspDI
BspHI BspLU11I BsrGI BssHII Bst1107I
BstBI BstZ17I ClaI DraI EcoRI
EcoRV FseI HpaI MamI MfeI
MluI MunI NdeI NotI NruI
NspV PacI PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI SalI SbfI SexAI
SfuI SgrAI SpeI Sse8387I SspBI
SspI SwaI Tth111I XbaI XmnI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
Acc65I 448 GGTACC G^GTACC
AflIII 3100 ACACGT A^C[GA][CT]GT
AhdI 5082 GACACACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 5275 GTGCAC G^TGCAC
ApaLI 5275 GTGCAC G^TGCAC
Asp718I 448 GGTACC G^GTACC
AspEI 5082 GACACACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 2499 TGCGCA TGC^GCA
BamHI 214 GGATCC G^GATCC
BbeI 1574 GGCGCC GGCGC^C
BciVI 2617 GTATCC GTATCC(6/5)
BglII 3173 AGATCT A^GATCT
BsiWI 1419 CGTACG C^GTACG
BssSI 2622 CACGAG CACGAG(-5/-1)
BstEII 3115 GGTGACC G^GT[ACGT]ACC
BstPI 3115 GGTGACC G^GT[ACGT]ACC
CpoI 311 CGGACCG CG^G[AT]CCG
Eam1105I 5082 GACACACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 3115 GGTGACC G^GT[ACGT]ACC
EcoT22I 4265 ATGCAT ATGCA^T
FspI 2499 TGCGCA TGC^GCA
HindIII 3163 AAGCTT A^AGCTT
KasI 1574 GGCGCC G^GCGCC
KpnI 448 GGTACC GGTAC^C
KspI 218 CCGCGG CCGC^GG
NaeI 196 GCCGGC GCC^GGC
NarI 1574 GGCGCC GG^CGCC
NgoMIV 196 GCCGGC G^CCGGC
NheI 2556 GCTAGC G^CTAGC
NsiI 4265 ATGCAT ATGCA^T
PaeR7I 5620 CTCGAG C^TCGAG
RsrII 311 CGGACCG CG^G[AT]CCG
SacII 218 CCGCGG CCGC^GG
ScaI 1108 AGTACT AGT^ACT
SfiI 2215 GGCCCCCCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 1574 GGCGCC GGC^GCC
SnaBI 1421 TACGTA TAC^GTA
XhoI 5620 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccII 62 CGCG CG^CG
219 CGCG
AccIII 1071 TCCGGA T^CCGGA
5504 TCCGGA
AflII 3166 CTTAAG C^TTAAG
3630 CTTAAG
AvrII 4824 CCTAGG C^CTAGG
5822 CCTAGG
BclI 3137 TGATCA T^GATCA
3902 TGATCA
BfrI 3166 CTTAAG C^TTAAG
3630 CTTAAG
BlnI 4824 CCTAGG C^CTAGG
5822 CCTAGG
BsaWI 1071 TCCGGA [AT]^CCGG[AT]
5504 TCCGGA
BseAI 1071 TCCGGA T^CCGGA
5504 TCCGGA
BsiEI 122 CGGCCG CG[GA][CT]^CG
194 CGGCCG
BspEI 1071 TCCGGA T^CCGGA
5504 TCCGGA
BstUI 62 CGCG CG^CG
219 CGCG
DrdI 1516 GACGAGATGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5837 GACAGCCCTGTC
EagI 122 CGGCCG C^GGCCG
194 CGGCCG
EclXI 122 CGGCCG C^GGCCG
194 CGGCCG
Eco52I 122 CGGCCG C^GGCCG
194 CGGCCG
FbaI 3137 TGATCA T^GATCA
3902 TGATCA
HincII 1041 GTCAAC GT[CT]^[GA]AC
2676 GTTGAC
HindII 1041 GTCAAC GT[CT]^[GA]AC
2676 GTTGAC
MroI 1071 TCCGGA T^CCGGA
5504 TCCGGA
MseI 3167 TTAA T^TAA
3631 TTAA
MvnI 62 CGCG CG^CG
219 CGCG
SacI 979 GAGCTC GAGCT^C
1067 GAGCTC
Tru9I 3167 TTAA T^TAA
3631 TTAA
Tsp509I 409 AATT ^AATT
576 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 135 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AlwNI AocI Aor51HI ApaI AspHI
AvaI AvaII BalI BanI BanII
BbsI BbvCI BbvI BcgI BcnI
BfaI BglI BlpI BmyI BpmI
Bpu1102I BpuAI BsaAI BsaHI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsmI
BsoBI Bsp1286I BspMI BsrBI BsrDI
BsrFI BsrI BssKI BstAPI BstF5I
BstNI BstXI BstYI Bsu36I Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DraIII DsaI EaeI
EarI Eco47III Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MslI MspA1I MspI MvaI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RsaI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
SmlI SphI StuI StyI TaqI
TfiI TseI Tsp45I TspRI TthHB8I
Van91I XcmI XhoII XmaCI XmaI