##################################
# NO CUT ENZYMES ( 66 enzymes) #
##################################
AatII AclI AflII AgeI AscI
AseI AsnI AviII BclI BfrI
BlpI Bpu1102I BsiEI BsiWI BsmBI
Bsp1407I BspDI BspLU11I BsrDI BsrGI
BssHII Bst1107I BstBI BstZ17I CelII
ClaI CpoI DraI DraIII DrdI
EagI EclXI Eco52I EcoRI EcoRV
FbaI FspI HpaI KspI MfeI
MunI NdeI NheI NotI NruI
NspV PacI PaeR7I PinAI PmeI
PshBI Psp1406I PvuI RsrII SacII
SapI SexAI SfiI SfuI SnaBI
SphI SspBI SspI SwaI XbaI
XhoI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
Acc65I 3082 GGTACC G^GTACC
AccII 3769 CGCG CG^CG
AccIII 904 TCCGGA T^CCGGA
AhdI 2406 GACACTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 2989 GAATTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 3082 GGTACC G^GTACC
AspEI 2406 GACACTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 3293 GGATCC G^GATCC
BbeI 3451 GGCGCC GGCGC^C
BglII 1075 AGATCT A^GATCT
BsaBI 267 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 904 TCCGGA T^CCGGA
BspEI 904 TCCGGA T^CCGGA
BstEII 2906 GGTGACC G^GT[ACGT]ACC
BstPI 2906 GGTGACC G^GT[ACGT]ACC
BstUI 3769 CGCG CG^CG
Eam1105I 2406 GACACTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 2906 GGTGACC G^GT[ACGT]ACC
FseI 3323 GGCCGGCC GGCCGG^CC
HincII 143 GTCGAC GT[CT]^[GA]AC
HindII 143 GTCGAC GT[CT]^[GA]AC
HindIII 549 AAGCTT A^AGCTT
KasI 3451 GGCGCC G^GCGCC
KpnI 3082 GGTACC GGTAC^C
MamI 267 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 3768 ACGCGT A^CGCGT
MroI 904 TCCGGA T^CCGGA
MvnI 3769 CGCG CG^CG
NarI 3451 GGCGCC GG^CGCC
PshAI 2961 GACCGGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SalI 143 GTCGAC G^TCGAC
ScaI 1097 AGTACT AGT^ACT
SfoI 3451 GGCGCC GGC^GCC
SgrAI 3795 CGCCGGCG C[GA]^CCGG[CT]G
SpeI 222 ACTAGT A^CTAGT
XmnI 2989 GAATTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccI 143 GTCGAC GT^[AC][GT]AC
3747 GTAGAC
AcyI 1792 GGCGTC G[GA]^CG[CT]C
3451 GGCGCC
AspI 287 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
882 GACCCAGTC
BbsI 468 GAAGAC GAAGAC(2/6)
654 GTCTTC
BcgI 3608 CGATCTATATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
236 GCAGAAGGATCG
BpuAI 468 GAAGAC GAAGAC(2/6)
654 GTCTTC
BsaHI 1792 GGCGTC G[GA]^CG[CT]C
3451 GGCGCC
BsaWI 904 TCCGGA [AT]^CCGG[AT]
4027 TCCGGT
BsmI 3107 GAATGC GAATGC(1/-1)
1978 GCATTC
BspHI 476 TCATGA T^CATGA
1681 TCATGA
BsrBI 1742 CCGCTC CCGCTC(-3/-3)
2469 GAGCGG
BstXI 359 CCATGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3271 CCAAGGAGCTGG
EcoT22I 1556 ATGCAT ATGCA^T
4219 ATGCAT
Hin1I 1792 GGCGTC G[GA]^CG[CT]C
3451 GGCGCC
MslI 1496 CACACCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
3090 CATCCAAGTG
NaeI 3324 GCCGGC GCC^GGC
3796 GCCGGC
NcoI 898 CCATGG C^CATGG
1501 CCATGG
NgoMIV 3324 GCCGGC G^CCGGC
3796 GCCGGC
NsiI 1556 ATGCAT ATGCA^T
4219 ATGCAT
NspI 1508 GCATGT [GA]CATG^[CT]
1554 ACATGC
PflFI 287 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
882 GACCCAGTC
PflMI 1584 CCAGTTAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2396 CCACTCACTGG
RcaI 476 TCATGA T^CATGA
1681 TCATGA
SacI 2895 GAGCTC GAGCT^C
4117 GAGCTC
SbfI 4124 CCTGCAGG CCTGCA^GG
4255 CCTGCAGG
Sse8387I 4124 CCTGCAGG CCTGCA^GG
4255 CCTGCAGG
StuI 3391 AGGCCT AGG^CCT
3427 AGGCCT
Tth111I 287 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
882 GACCCAGTC
Van91I 1584 CCAGTTAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2396 CCACTCACTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 127 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
Alw44I AlwI AlwNI AocI Aor51HI
ApaI ApaLI ApoI AspHI AvaI
AvaII AvrII BalI BanI BanII
BbrPI BbvCI BbvI BciVI BcnI
BfaI BglI BlnI BmyI BpmI
BsaAI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrFI BsrI
BssKI BssSI BstAPI BstF5I BstNI
BstYI Bsu36I Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco47III Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PleI PmaCI PmlI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XhoII
XmaCI XmaI