##################################
# NO CUT ENZYMES ( 73 enzymes) #
##################################
AatII AccIII AclI AflII AflIII
AhdI Alw44I Aor51HI ApaLI AscI
AseI AsnI Asp700I AspEI BbrPI
BciVI BclI BfrI BsaAI BseAI
BsiWI Bsp1407I BspDI BspEI BspLU11I
BsrGI BssHII Bst1107I BstBI BstZ17I
ClaI CpoI Eam1105I Eco47III EcoT22I
FbaI FseI KspI MluI MroI
NaeI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RsrII SacII SalI
SbfI ScaI SexAI SfiI SfuI
SgrAI SnaBI Sse8387I SspBI SwaI
XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AgeI 1755 ACCGGT A^CCGGT
AocI 3492 CCTTAGG CC^T[ACGT]AGG
ApaI 1212 GGGCCC GGGCC^C
AviII 1035 TGCGCA TGC^GCA
AvrII 3186 CCTAGG C^CTAGG
BbsI 3046 GTCTTC GAAGAC(2/6)
BcgI 3728 GCAGGAGAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 987 GCCTCCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1047 AGATCT A^GATCT
BlnI 3186 CCTAGG C^CTAGG
BlpI 741 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 741 GCTCAGC GC^T[ACGT]AGC
BpuAI 3046 GTCTTC GAAGAC(2/6)
BsaBI 613 GATCATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1755 ACCGGT [AT]^CCGG[AT]
BsmI 53 GCATTC GAATGC(1/-1)
BspHI 595 TCATGA T^CATGA
BspMI 1179 ACCTGC ACCTGC(4/8)
BsrFI 1755 ACCGGT [GA]^CCGG[CT]
BssSI 1025 CTCGTG CACGAG(-5/-1)
Bsu36I 3492 CCTTAGG CC^T[ACGT]AGG
CelII 741 GCTCAGC GC^T[ACGT]AGC
Cfr10I 1755 ACCGGT [GA]^CCGG[CT]
DraIII 2273 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2148 GACCTGATGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 3492 CCTTAGG CC^T[ACGT]AGG
EcoRV 3081 GATATC GAT^ATC
FspI 1035 TGCGCA TGC^GCA
HgaI 1339 GACGC GACGC(5/10)
HincII 2950 GTTAAC GT[CT]^[GA]AC
HindII 2950 GTTAAC GT[CT]^[GA]AC
HpaI 2950 GTTAAC GTT^AAC
MamI 613 GATCATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 2317 CAATTG C^AATTG
MunI 2317 CAATTG C^AATTG
NcoI 991 CCATGG C^CATGG
PinAI 1755 ACCGGT A^CCGGT
RcaI 595 TCATGA T^CATGA
SmaI 351 CCCGGG CCC^GGG
SpeI 2181 ACTAGT A^CTAGT
SphI 1821 GCATGC GCATG^C
SspI 3984 AATATT AAT^ATT
StuI 999 AGGCCT AGG^CCT
XmaCI 351 CCCGGG C^CCGGG
XmaI 351 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AccI 210 GTAGAC GT^[AC][GT]AC
548 GTCTAC
AcyI 573 GGCGCC G[GA]^CG[CT]C
2492 GGCGCC
AlwNI 725 CAGCGCCTG CAG[ACGT][ACGT][ACGT]^CTG
2223 CAGAGACTG
AspI 1064 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1943 GACTCCGTC
AvaI 351 CCCGGG C^[CT]CG[GA]G
1352 CCCGAG
BamHI 409 GGATCC G^GATCC
1503 GGATCC
BbeI 573 GGCGCC GGCGC^C
2492 GGCGCC
BsaHI 573 GGCGCC G[GA]^CG[CT]C
2492 GGCGCC
BsaI 1752 GAGACC GGTCTC(1/5)
2565 GGTCTC
BsiEI 663 CGGCCG CG[GA][CT]^CG
762 CGGCCG
BsoBI 351 CCCGGG C^[CT]CG[GA]G
1352 CCCGAG
BsrBI 1216 CCGCTC CCGCTC(-3/-3)
1085 GAGCGG
BsrDI 2407 GCAATG GCAATG(2/0)
4010 GCAATG
BstAPI 2203 GCAGCTTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3431 GCAAATAATGC
BstEII 1005 GGTCACC G^GT[ACGT]ACC
3693 GGTCACC
BstPI 1005 GGTCACC G^GT[ACGT]ACC
3693 GGTCACC
DsaI 991 CCATGG C^C[GA][CT]GG
1608 CCGTGG
EagI 663 CGGCCG C^GGCCG
762 CGGCCG
EclXI 663 CGGCCG C^GGCCG
762 CGGCCG
Eco52I 663 CGGCCG C^GGCCG
762 CGGCCG
EcoNI 1060 CCTGGACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1180 CCTGCACCAGG
EcoO65I 1005 GGTCACC G^GT[ACGT]ACC
3693 GGTCACC
EcoRI 1382 GAATTC G^AATTC
2169 GAATTC
Hin1I 573 GGCGCC G[GA]^CG[CT]C
2492 GGCGCC
HindIII 1684 AAGCTT A^AGCTT
3065 AAGCTT
KasI 573 GGCGCC G^GCGCC
2492 GGCGCC
MaeII 252 ACGT A^CGT
3400 ACGT
NarI 573 GGCGCC GG^CGCC
2492 GGCGCC
PflFI 1064 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1943 GACTCCGTC
PpuMI 486 AGGACCC [GA]G^G[AT]CC[CT]
2124 AGGTCCC
PvuII 1838 CAGCTG CAG^CTG
3954 CAGCTG
SapI 889 GAAGAGC GCTCTTC(1/4)
1017 GCTCTTC
SfoI 573 GGCGCC GGC^GCC
2492 GGCGCC
Tth111I 1064 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1943 GACTCCGTC
XcmI 900 CCAGCCAGACGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3284 CCAGTCTATGGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 105 enzymes) #
#########################################
Acc65I AccII AciI AcsI AfaI
AluI AlwI ApoI Asp718I AspHI
AvaII BalI BanI BanII BbvCI
BbvI BcnI BfaI BmyI BpmI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI BstUI
BstXI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraI DraII EaeI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI KpnI Ksp632I MaeI MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PflMI PleI
PstI RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XhoII