##################################
# NO CUT ENZYMES ( 80 enzymes) #
##################################
AatII AccI AflII AgeI Alw44I
AlwI AocI Aor51HI ApaLI AscI
AviII BalI BamHI BbeI BcgI
BciVI BclI BfrI BglII BsiWI
BspDI BspHI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstYI
BstZ17I Bsu36I ClaI CpoI DrdI
EagI EclXI Eco47III Eco52I Eco81I
EcoO65I FbaI FseI FspI KasI
MflI MluI MluNI MscI NarI
NdeI NotI NruI NspV PacI
PaeR7I PinAI PmeI PshAI PvuI
RcaI RsrII SalI SapI SbfI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SwaI
XbaI XhoI XhoII XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
###################################
Acc65I 3537 GGTACC G^GTACC
AccIII 37 TCCGGA T^CCGGA
AclI 1544 AACGTT AA^CGTT
AcyI 123 GACGCC G[GA]^CG[CT]C
AflIII 754 ACATGT A^C[GA][CT]GT
AhdI 2097 GACAGGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 2628 CAGAATCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 408 GGGCCC GGGCC^C
AseI 2888 ATTAAT AT^TAAT
AsnI 2888 ATTAAT AT^TAAT
Asp700I 3159 GAAAATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 3537 GGTACC G^GTACC
AspEI 2097 GACAGGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 1418 GAGCTC G[AT]GC[AT]^C
AvrII 1857 CCTAGG C^CTAGG
BbrPI 1196 CACGTG CAC^GTG
BbvCI 1975 CCTCAGC CCTCAGC(-5/-2)
BlnI 1857 CCTAGG C^CTAGG
BlpI 1651 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 1651 GCTTAGC GC^T[ACGT]AGC
BsaAI 1196 CACGTG [CT]AC^GT[GA]
BsaBI 3493 GATTTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 123 GACGCC G[GA]^CG[CT]C
BseAI 37 TCCGGA T^CCGGA
BsiEI 704 CGGTCG CG[GA][CT]^CG
BsiHKAI 1418 GAGCTC G[AT]GC[AT]^C
BsmBI 913 GAGACG CGTCTC(1/5)
BspEI 37 TCCGGA T^CCGGA
BspLU11I 754 ACATGT A^CATGT
BspMI 2412 ACCTGC ACCTGC(4/8)
BsrBI 920 GAGCGG CCGCTC(-3/-3)
CelII 1651 GCTTAGC GC^T[ACGT]AGC
DraIII 2491 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 2097 GACAGGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 2976 GAATTC G^AATTC
EcoRV 306 GATATC GAT^ATC
HaeII 82 AGCGCC [GA]GCGC^[CT]
HgaI 123 GACGC GACGC(5/10)
Hin1I 123 GACGCC G[GA]^CG[CT]C
HpaI 572 GTTAAC GTT^AAC
KpnI 3537 GGTACC GGTAC^C
MamI 3493 GATTTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 3107 CAATTG C^AATTG
MroI 37 TCCGGA T^CCGGA
MunI 3107 CAATTG C^AATTG
NaeI 165 GCCGGC GCC^GGC
NgoMIV 165 GCCGGC G^CCGGC
NheI 98 GCTAGC G^CTAGC
PmaCI 1196 CACGTG CAC^GTG
PmlI 1196 CACGTG CAC^GTG
PpuMI 709 GGGTCCT [GA]G^G[AT]CC[CT]
PshBI 2888 ATTAAT AT^TAAT
Psp1406I 1544 AACGTT AA^CGTT
SacI 1418 GAGCTC GAGCT^C
ScaI 1146 AGTACT AGT^ACT
XcmI 1159 CCAGCAGGCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 3159 GAAAATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AvaI 361 CCCGAG C^[CT]CG[GA]G
2017 CCCGAG
BanII 408 GGGCCC G[GA]GC[CT]^C
1418 GAGCTC
BbsI 2184 GTCTTC GAAGAC(2/6)
2642 GTCTTC
BglI 165 GCCGGCCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1434 GCCGCAAAGGC
BpuAI 2184 GTCTTC GAAGAC(2/6)
2642 GTCTTC
BsaWI 37 TCCGGA [AT]^CCGG[AT]
228 ACCGGA
BsmI 1255 GCATTC GAATGC(1/-1)
2585 GCATTC
BsoBI 361 CCCGAG C^[CT]CG[GA]G
2017 CCCGAG
Bsp1407I 579 TGTACA T^GTACA
4073 TGTACA
BsrDI 3250 CATTGC GCAATG(2/0)
3447 GCAATG
BsrFI 129 GCCGGT [GA]^CCGG[CT]
165 GCCGGC
BsrGI 579 TGTACA T^GTACA
4073 TGTACA
BstXI 828 CCAAAGAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2556 CCAAGTATCTGG
Cfr10I 129 GCCGGT [GA]^CCGG[CT]
165 GCCGGC
DraII 407 AGGGCCC [GA]G^G[ACGT]CC[CT]
709 GGGTCCT
EarI 2469 CTCTTC CTCTTC(1/4)
808 GAAGAG
EcoNI 1215 CCTCAAATAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1315 CCTGAAAAAGG
EcoO109I 407 AGGGCCC [GA]G^G[ACGT]CC[CT]
709 GGGTCCT
EcoT22I 2149 ATGCAT ATGCA^T
2614 ATGCAT
HincII 572 GTTAAC GT[CT]^[GA]AC
3835 GTTGAC
HindII 572 GTTAAC GT[CT]^[GA]AC
3835 GTTGAC
HphI 825 TCACC GGTGA(8/7)
3874 TCACC
Ksp632I 2469 CTCTTC CTCTTC(1/4)
808 GAAGAG
KspI 6 CCGCGG CCGC^GG
28 CCGCGG
NcoI 669 CCATGG C^CATGG
3764 CCATGG
NsiI 2149 ATGCAT ATGCA^T
2614 ATGCAT
PflMI 1039 CCATTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2490 CCACTGGGTGG
PvuII 884 CAGCTG CAG^CTG
1056 CAGCTG
SacII 6 CCGCGG CCGC^GG
28 CCGCGG
SphI 1344 GCATGC GCATG^C
3420 GCATGC
SspBI 579 TGTACA T^GTACA
4073 TGTACA
TaqI 747 TCGA T^CGA
775 TCGA
TthHB8I 747 TCGA T^CGA
775 TCGA
Van91I 1039 CCATTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2490 CCACTGGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 87 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
ApoI AspI AvaII BanI BbvI
BcnI BfaI BmyI BpmI BsaI
BsaJI BseRI BsgI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrI BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr13I DdeI DpnII DraI DsaI
EaeI EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HindIII HinfI HpaII ItaI MaeI
MaeII MaeIII MboI MboII MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PflFI PleI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspI StuI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Tth111I